Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0393754 (
HSA
)
2,996
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The binding mechanism of human serum albumin with 3,3', 4', 7-tetradroxyflavone (
FIS
) was characterized by fluorescence and UV absorption spectra. The intrinsic fluorescence of
HSA
was significantly quenched by
FIS
in the physiological condition (pH 7.4), and the quenching mechanism is mainly static quenching process in the drug to protein molar ratio ranging from 0.1 to 10. The results revealed that the drug and protein formed 1 : 1 complex with the binding constants of (1.05 +/- 0.18) x 10(5) L x mol(-1). The dissociation behaviors of
FIS
in different pH conditions were investigated by UV absorption spectra, and it was found that
FIS
existed in the mixtures of ionic and neutral species. The UV absorption band I of
FIS
has a significant red shift (above 40 nm) after combination with
HSA
, demonstrating that
FIS
was bound to protein in ionic species which was driven by electrostatic force. The second derivative spectra of
FIS
showed that the combination included specific and non-specific forms. The intrinsic fluorescence of
FIS
was conspicuously enhanced in the presence of
HSA
due to the excited-state proton transfer (ESPT) and this further confirmed the complex formation of
HSA
with
FIS
.
...
PMID:[Spectroscopic characterization of binding between human serum albumin and 3,3', 4',7-tetrahydroxyflavone]. 1842 34
