Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0393754 (HSA)
 2,996 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To study the binding of rutin and serum albumin (SA) in physiological condition and the quenching mechanism of the fluorescence of SA by rutin, the fluorescence method was used. The results shows that the emission spectra of BSA (HSA) in the presence and absence of rutin are different. The emission spectra of BSA (HSA) in the presence of rutin can be quenched. The quenching mechanism of rutin to SA was static quenching with non-radiation energy transfer with single molecule. The binding constants K(A), the number of binding sites n and the thermodynamic parameters of the reaction of rutin with SA were determined at different temperatures. At 295 and 310 K, for BSA, K(A)(L * moL(-1)) = 4.215 x 10(4) and 6.996 x 10(3) and n = 0.75 and 0.64, respectively; for HSA, K(A)(L * moL (-1)) = 2.660 x 10(4) and 4.110 x 10(3) and n = 0.70 and 0.60, respectively. The binding constants K(A) decreased with the increase in temperature, which means that rutin and SA have a quite strong ability to form a new complex-system. The main binding force was discussed by thermodynamic equation, and the result is that deltaH < 0 and deltaS < 0 for the reaction of rutin with SA. So the binding forces was mainly H-bond and Van der Waals. The effect of the drug on the conformation of serum albumin was also studied by using synchronous fluorescence spectroscopy. Rutin could be deposited and transported by serum protein in vivo. Rutin had nearly no effect on the serum protein conformation.
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PMID:[Thermodynamics studies on the binding of rutin and serum albumin]. 1861 15

This work was designed to develop a simple, effective, and reliable LC system to identify a chemical marker and compare Sambucus nigra L. and Sambucus australis Cham. et Schltdl. flower extracts (American and European elder). Rutin was the main constituent of both species. The developed method showed a linear response in the range of 10 to 45 microg x mL(-1) for rutin and 1.75 to 3.25 microg x mL(-1) for samples of the Sambucus species. Precision was determined and the relative standard deviations were 1.75 % for HSN and 1.28 % for HSA for intraday precision and 1.28 % and 1.51 % for inter-day precision, respectively, while accuracy was 97.9 % for HSN and 99.41 % for HSA. Quantification and detection limits as well as robustness were determined, presenting adequate results. The LC method showed an adequate performance for the separation of flavonoid glycosides in S. nigra and S. australis extracts, since the presence of interference had been previously evaluated. The analysis of thirty different samples of S. NIGRA and S. australis of different origins did not show significant variability among them. An accelerated stability study revealed a significant decrease in the first 30 days reaching 57 % in 90 days for S. australis samples and a total decrease of 25 % in 90 days for S. nigra samples, considering rutin as the chemical marker. These results will contribute to quality control analysis routines of these raw materials in pharmaceutical production facilities.
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PMID:Comparative analysis of Sambucus nigra and Sambucus australis flowers: development and validation of an HPLC method for raw material quantification and preliminary stability study. 2019 57