Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0393754 (HSA)
 2,996 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have to date installed artificial spermatoceles made of silicone in a total of 33 patients. Artificial insemination of husband (AIH) was performed in 1-9 times using semen collected from 11 of the 33 patients in whom spermatocele was installed, and pregnancy was achieved in 2 cases. Reported cases of pregnancy achieved using semen collected from artificial spermatoceles are few in number; indeed, our successful cases were the fifth and sixth reported so far. The key point for success in achieving pregnancy by means of the artificial spermatocele is considered to be attempting AIH as soon as possible after collecting the sperm, because collection of sperm soon becomes impossible, due to early obstruction of the incised region at the epididymis by degeneration of fibrous tissue. 3% HSA-TMPA medium was found to be useful for sperm collection.
 ...
PMID:Clinical experience and successful impregnation using an artificial spermatocele. 177 3

Among the monoclonal antibodies generated against acrosome-reacted human sperm, HSA-5 was shown to react with a sperm antigen localized predominantly to the equatorial region of the acrosome of human sperm and to the head and tail of mouse sperm. This antibody reacted with the methanol-fixed sperm, but not with fresh live sperm. When purified by immunoaffinity column, a major protein band with a molecular mass of approximately 100 kDa on SDS gel was isolated from fresh human sperm extract. The immunospecificity of isolated human sperm protein to this monoclonal antibody was verified by enzyme-linked immunosorbent assay and Western blot analysis. This antigen, designated as HSA-5, was susceptible to proteolytic degradation and revealed multiple immunoreactive bands in Western blot analyses of some preparations. Mouse sperm homogenates showed a similar polymorphic pattern to that of human samples. The tissue specificity of this antigen was examined immunohistochemically using various mouse and human tissues. HSA-5 did not cross-react with any other tissues except for sperm in adult testes and epididymis. This antibody also showed no binding activity to testicular tissue sections from mice of 13 and 21 days of age. The results of our study suggest that the sperm antigen recognized by HSA-5 monoclonal antibody is a differentiation antigen, which is expressed postmeiotically in testicular sperm but not in any somatic tissues.
 ...
PMID:Purification and characterization of a sperm antigen recognized by HSA-5 monoclonal antibody. 863 26

Fibronectin type II (Fn2) module-containing proteins in the male genital tract are characterized by different numbers of Fn2 modules. Predominantly two classes exist which are distinct by having either two or four Fn2 modules. Minor variants with three Fn2 modules were also found in the human and the porcine epididymis. To reveal their relationship, mRNAs and proteins of representatives of these classes were studied in human, in Sus scrofa, and in rodents. Adult boars expressed members of both classes, i.e. ELSPBP1 and pB1, in subsequent regions of the epididymis, and both were under androgenic control. Human and rodent epididymides, on the other hand, alternatively contained only representatives of one of these two classes, i.e. ELSPBP1 in the human and two different pB1-related counterparts in rodents. ELSPBP1 and pB1-related genomic sequences were closely linked in chromosomal regions HSA 19q and SSC 6 q11-q21; conserved synteny between these regions is well established. On the other hand, in a syntenic region on mouse chromosome 7, ELSPBP1-related sequences were lacking. Tight binding to the sperm membrane via a choline-mediated mechanism was a common feature of the two classes of Fn2-module proteins, suggesting related function(s). However, differences in their regionalized expression patterns along the male genital tract as well as in association sites on the sperm surface suggested a species-specific sequential order in sperm binding.
 ...
PMID:Sperm-binding fibronectin type II-module proteins are genetically linked and functionally related. 1730 9