Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0393754 (HSA)
 2,996 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Twelve acyclic mares of various ages (2-29 years) and parity (maiden-multiparous) were given oestradiol-17 beta i.m. (winter 1982) or progesterone i.m. (winter 1983) to induce changes in the endometrium consistent with oestrus and dioestrus, respectively. After hormone treatment, mares were inoculated intrauterine with 50 ml saline containing 5 X 10(5) Streptococcus zooepidemicus bacteria, 51Cr-labelled 15-micron microspheres, and 500 mg charcoal (Groups E + B and P + B) or microspheres and charcoal only (Groups EC and PC). At 5 h after inoculation uteri were flushed with 50 ml saline containing tracer amounts of 125I-labelled HSA. In Group E + B inoculated materials were cleared more rapidly in younger than in older mares, and there was a significant positive correlation between age and bacterial concentration, total numbers of bacteria, and amounts of microspheres and charcoal in the uterus and also between age and the WBC concentration and total numbers of WBC. In Group EC there was a significant positive correlation between age and the amounts of microspheres and charcoal remaining in the uterus, and between age and WBC concentration and total numbers of WBC. We suggest that in the oestrogen-dominated uterus physical drainage may be a factor in determining whether a mare is 'resistant' or 'susceptible' to bacterial challenge of the uterus. Physical clearance is increased in younger (resistant) mares in the presence and absence of an antigenic stimulus. In mares in Group P + B there was no correlation between age and the values measured.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Factors affecting uterine clearance of inoculated materials in mares. 331 42

In the near future, 6 of 8 bear species will face extinction mainly because of loss of their natural habitat. This loss of habitat will ultimately require some of these bears to be maintained in zoos and wildlife preserves in the hope of conserving genetic diversity. If the giant panda is representative of other bear species, reproductive performance will be inhibited in such an environment. In this study, we used the nonendangered American black bear (Ursus americanus) as the model for developing appropriate embryo transfer procedures. The donor bear mated numerous times between late May and early June. In late July we anesthetized her and used a series of telescoping sheaths to gain access to the uterus Then we passed a catheter through the largest sheath, inflated the balloon, and, using a 20-mL syringe, repeatedly infused into and then aspirated from the uterus PBS + BSA. We emptied the syringe into Petri dishes and observed 2 embryos. We rinsed the embryos, placed them in human tubal fluid + HSA + HEPES and then held them at 35 degrees C for 5 h. The recipient mated during mid-June; in late July we anesthetized her and, with the aid of laparoscopy, transferred an embryo into the cranial portion of the uterine horn ipsilateral to the ovary containing a CL. The recipient delivered 2 cubs in January. Necropsy results indicated that the neonates lived for 6 to 8 wk before succumbing to flooding in the den. The DNA from hair samples belonging to the neonates indicated that the male cub belonged to the donor, the female cub to the recipient. The delayed implantation mechanism in bears probably allowed for the successful development of the embryo in the presence of a substantial asynchrony between the donor and the recipient (13 d). We conclude that embryo transfer is possible in the American black bear and can lead to the birth of live cubs.
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PMID:Live birth of a bear cub following nonsurgical embryo collection. 1072 38

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid-oestrus, late oestrus and interoestrus or a radiopharmaceutical ((99m)Tc-HSA) during mid- and interoestrus in a non-ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid-oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol-17beta concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid-oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral-shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.
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PMID:Investigation of cervical patency and uterine appearance in domestic cats by fluoroscopy and scintigraphy. 1235 79