Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0392674 (
exhaustion
)
13,658
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In this study, we planned to investigate the function and potential mechanisms of Alpha-1,3-mannosyltransferase (
ALG3
) in oral squamous cell carcinoma (OSCC). Data from TCGA were used to analyze
ALG3
expression and its effect on the prognosis of patients with OSCC. KEGG enrichment analysis was applied to explore the pathways related to
ALG3
.
ALG3
expression was measured by qPCR and Western blot. Cell counting kit-8, colony formation, and transwell assays were implemented to detect the effects of
ALG3
on malignant biological properties of OSCC cells. The expression of key proteins related to CDK-Cyclin pathway was detected by Western blot. The expression of
ALG3
in OSCC samples was higher than that of the control samples, and the increase of
ALG3
expression was related to unfavorable prognosis of OSCC patients. Additionally, the elevated expression of
ALG3
was associated with pathological stage, lymph node metastasis, and primary lesion in OSCC patients.
ALG3
depletion blocked the growth and movement of OSCC cells, while over-expression
ALG3
reversed these phenomena. Moreover,
exhaustion
of
ALG3
resulted in decreased expression of MCM7/CCNB2/CDK1/PCNA, while these phenomena were inversed after
ALG3
up-regulation. The enhancement of
ALG3
expression promoted the aggressive biological behaviors of OSCC cells probably by promoting CDK-Cyclin pathway.
...
PMID:ALG3 contributes to the malignant properties of OSCC cells by regulating CDK-Cyclin pathway. 3308 11
