Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0348321 (
Haemophilus
)
15,372
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Bacillus amyloliquefaciens cells were found to contain an exonuclease which catalyzes the sequential hydrolysis of mononucleotides from the 3'-termini of duplex DNA. The enzyme was purified to homogeneity and its molecular weight (as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate) is 29,000. The exonuclease possesses an additional catalytic activity, i.e.,
3'-5' exonuclease
specific for the RNA strand in an RNA--DNA hybrid duplex (RNase H activity). In terms of physical and catalytic properties the exonuclease of B. amyloliquefaciens is similar to exonucleases III from E. coli and
Haemophilus
influenzae and can thus be related to the same class of hydrolases, i.e., 3.1.11.2. However, in comparison with exo III from E. coli, the enzyme from B. amyloliquefaciens exhibits a more strict specificity for the structure of the substrate 3'-end.
...
PMID:[Exonuclease III from Bacillus amyloliquefaciens]. 331 Nov 77
