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Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A broth system was developed for rapid identification of the requirement for X factor (hemin), or V factor (NAD), or both for growth of Haemophilus species. This system was compared to growth around paper discs/strips impregnated with factors X and/or V. The broth system consisted of three tubes, each containing brain-heart infusion broth supplemented with V factor, X factor, or both. Each tube was inoculated with a saline suspension of an Haemophilus isolate, and the broths were shaken for aeration at 37 degrees C. Under these conditions turbidity or clumping was usually evident after 4-5 hr only in the broth(s) containing the required supplement(s). A few strains requiring only V factor required overnight incubation. One hundred fifty-six Haemophilus isolates were tested for growth around supplemented discs/strips or in supplemented broths: 129 were H. influenzae/aegypticus, 25 were of various species that required only V factor, and 2 were H. aphrophilus. Ten of 89 H. influenzae isolates from the respiratory tract were misidentified by satellitism. All isolates were correctly identified by growth in supplemented broths. The cost of the broth assay was about 60 cents/test, whereas the satellite assay cost about 120 cents/test. Serotyping and antibiotic sensitivity testing could be performed directly from the broth culture. Determination of X and/or V requirement by Haemophilus species with supplemented broths was sensitive, rapid, and inexpensive.
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PMID:Rapid determination of X/V growth requirements of Haemophilus species in broth. 349 13

Some solid media for antigen preparation of Haemophilus paragallinarum (HPG) hemagglutinin (HA) were studied. When HA activities of HPG strain 221 cultivated on chicken meat infusion (CMI) and gonococcal (GC) agar media were compared, the titer of the GC agar medium appeared faster and was higher than the titer on the CMI agar medium. For both agar media, the HA titer and growth increased with the concentration of the chicken serum added. In addition, HA activity on GC agar medium showed a higher titer than activity on other media, such as brain-heart infusion, Trypticase soy, blood, and Kato's agar media. When various HPG strains were inoculated onto GC agar medium, HA titers in strains 0083 and F were equal to that in strain 221. These results confirm that the GC agar medium is useful for preparation of HA antigen of HPG.
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PMID:Use of gonococcal agar medium for preparation of antigen of Haemophilus paragallinarum hemagglutinin. 709 40

A chemically defined medium that supports the growth of both encapsulated and nontypeable Haemophilus influenzae strains in broth to densities that are >/= 10(9) CFU/ml or on agar plates is described. The mean generation time of a panel of clinical isolates was comparable to that in rich, chemically undefined media (brain-heart infusion broth supplemented with heme and beta-NAD).
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PMID:Chemically defined media for growth of Haemophilus influenzae strains. 1295 78