Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Immunization with ribosomal preparations from Haemophilus influenzae type b elicited protective immunity in mice. Ribosomes from disrupted cells where isolated by differential centrifugation using sodium dodecyl sulfate. The washed ribosomes contained 25% protein and 75% ribonucleic acid and sedimented as a single peak on sucrose density gradient analysis with a sedimentation coefficient of 67S, using Escherichia coli ribosomes as a 70S marker. Immunodiffusion tests with antipolyribose phosphate serum showed that the ribosomes were free from capsular material. Mice immunized subcutaneously with ribosomes, with or without adjuvant, were challenged intraperitoneally with 100 to 1,000 mean lethal doses of H. influenzae type b suspended in gastric mucin. Significant protection was induced by ribosomes and was compared to that obtained after sublethal infection with live cells. The protection was greatly enhanced after incorporation of ribosomes into adjuvants. Maximum protection (90 to 95%) was observed at 1 to 2 weeks after immunization. Ribosomes from a nonencapsulated strain of H. influenzae were as immunogenic as those from the encapsulated strain, demonstrating that the capsular material is not responsible for immunogenicity of Haemophilus ribosomes.
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PMID:Immunoprotective activity of ribosomes from Haemophilus influenzae. 30 Mar 60

The kinetics of infection was studied in normal and ribosome-immunized mice challenged with Haemophilus influenzae Type b organisms. Ribosomal preparations extracted by the differential-centrifugation and sodium-dodecyl-sulphate treatment or ammonium-sulphate-precipitation procedures were highly immunoprotective when mice were challenged by the i.p. route. After i.p. injections, organisms rapidly spread to blood, liver, lungs and brain in normal and immunized mice. However, by 24 h after injection, evidence of organism clearance could be seen in immunized mice. By 32 h organisms were cleared from blood, brain and lungs of all immunized mice and from spleens in 2 of 3 mice. However, organisms persisted in high numbers of unimmunized mice until their death by 48 h. These data indicate that i.p. injections of H. influenzae mixed with gastric mucin leads to a true infection and can be used as a model to evaluate immunoprotective activity. The kinetics of infection induced by intracerebral (i.c.) inoculation also was studied. The LD50 for this type of infection was more than 1000 times the LD50 for i.p. infection. The patterns of infection induced by i.c. challenge were similar in normal and immunized mice and immunoprotection could not be detected using this model.
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PMID:Kinetics of Haemophilus influenzae type B infection in normal and ribosome-immunized mice using intraperitoneal and intracerebral routes of inoculation. 697 13