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Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The sites on the left arm of bacteriophage lambda DNA cleaved by the restriction endonucleases isolated from Hemophilus influenzae strain Rc (HincII) and Rd (HindII + III), and Hemophilus parainfluenzae (HpaI) were localized on the lambda physical map, and the fragments resulting from these cleavages were identified by gel electrophoresis. The restriction sites within the b2 region of lambda were mapped by analysis of the digestion profiles of deletion and substitution derivatives of lambda, as well as by digesting individual fragments produced by one restriction endonuclease with another restriction endonuclease. The restriction sites of the lambda genome between the left vegetative end and the b2 region were mapped entirely by succesive digestion experiments. The restriction fragment map for the right arm of lambda may be found in the accompanying paper (Robinson and Landy, 1977).
Gene 1977 Sep
PMID:HindII, HindIII, and HpaI restriction fragment maps of the left arm of bacteriophage lambda DNA. 59 5

Using positive blood, lung, or pleural fluid cultures as definitive criteria for bacterial infection, 43 examples of Hemophilus influenzae type b pneumonia were identified in a 43-month period. The mean age of the patients was 26 months; 12% were older than 5 years of age. Associated infections were found in 34 patients and included upper respiratory infections, otitis media, epiglottitis, and meningitis. Positive nasopharyngeal cultures were observed in only 33%. Radiologically, segmental or lobar infiltrates accounted for 85% of the pneumonias. In two cases, death was attributed to the pneumonia alone. Treatment with penicillin G or ampicillin was equally effective. Our data suggest that H. influenzae pneumonia is commonly a serious infection that cannot be distinguished clinically or radiologically from other pneumonias.
J Pediatr 1978 Sep
PMID:Hemophilus influenzae type b pneumonia in 43 children. 69 Jul 52

The cleavage of DNA by restriction endonucleases HpaII and HapII is prevented by the presence of a 5-methyl group at the internal C residue of its recognition sequence CCGG. MspI, an isoschizomer of HpaII available from New England Biolabs, cleaves DNA irrespective of the presence of a methyl group at this position. This enzyme cleaves DNA from Haemophilus parainfluenzae and Haemophilus aphrophilus readily while HpaII and HapII cannot degrade these DNAs. Practically all HpaII sites in mammalian sperm DNA are also protected by methylation at the internal C position since HpaII and HapII barely cleave this DNA (average molecular weight 40 kb). MspI, however, cleaves the DNA to an average size of about 5 kb.
Nucleic Acids Res 1978 Sep
PMID:MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites. 70 54

The restriction endonuclease from Haemophilus parainfluenzae, endoR.HpaI cleaves lambdacI857s7 DNA into 14 fragments. The sizes of these fragments were determined and a physical map was constructed. The ordering of the fragments was carried out using different deletion and substitution mutants of lambda phage, double cleavages with another restriction enzyme, endoR.BamHI, and partial protection of individual HpaI recognition sites by the antibiotics distamycin A and actinomycin D. HpaI produces fragments from the left arm of the lambda DNA genome, which may help in investigating the structure and function of this part of the phage.
Gene 1978 Sep
PMID:Physical mapping of bacteriophage lambda DNA with restriction endonuclease HpaI. 73 55

Replicative form DNA of bacteriophage M13 was cleaved into specific fragments by an endonuclease isolated from Hemophilus aegyptius (endoR.HaeII) and an endonuclease from Arthrobacter luteus (endoR.AluI). The fragments were ordered as to construct a circular map of the phage M13 genome by: (a) using each fragment as a primer for the synthesis in vitro of its respective neighbour and (b) digesting the isolated fragments with the Hemophilus aegyptius enzyme endoR.HaeII or the Hemophilus aphirophilus enzyme endoR.HapII and subsequent analysis of the overlapping sets of fragments. The resulting physical map was correlated with the M13 genetic map by marker rescue experiments with amber mutant phage DNAs and purified wild-type fragments. From the results of these analyses it has been concluded that gene II and gene V are contiguous on the genetic map. Evidence is provided that there is an internal start of RNA synthesis within the C-terminal region of gene II which then ultimately leads to the synthesis of X protein. Furthermore, we conclude that there is an intergenic space of considerable length (450-500 base pairs) which is located between gene II and gene IV on the M13 genome. The function of this intergenic region as the origin site for phage DNA replication is discussed.
Eur J Biochem 1976 Sep
PMID:Restriction-enzyme-cleavage maps of bacteriophage M13. Existence of an intergenic region on the M13 genome. 78 38

The clinical and microbiological features of a case of Haemophilus aphrophilus endocarditis in pregnancy are described. The complicating effect of pregnancy on treatment and the difficulties in identifying the organism in the laboratory are discussed.
J Clin Pathol 1976 Sep
PMID:Haemophilus aphrophilus endocarditis in pregnancy. 78 9

Calf thymus DNA was digested with the restriction nucleases from Escherichia coli carrying resistance transfer factor I, Haemophilus influenzae Rd and Bacillus subtilis X5 (EcoRI, Hind II, and Bsu, respectively) and submitted to polyacrylamide gel electrophoresis. About 10% of the DNA migrated as discrete fragments in 8, 16, and 30 bands, respectively, superimposed upon a continuous distribution of various size DNA fragments. The fragments within the bands are repeated 2000 to 160000 times in the haploid genome. Their sizes range from about 10 to a few thousand nucleotide pairs. About 5% of the DNA in EcorRI and Hind II digests migrated in a band at the position of undigested DNA, probably due to the resistance of long stretches of DNA against these nucleases. Calf DNA fragments obtained with EcoRI and Hind II were isolated by preparative gel electrophoresis. DNA from the bands showed the behaviour of repetitive DNA in renaturation experiments. An EcoRI fragment 1300-nucleotide-pairs long, which represents 6% of the calf genome and occurs 130000 times, is tandemly repeated (derived from the satellite of 1.714 g/cm3, see below). Another EcoRI fragment of 970 nucleotide paris, which represents 0.5% of the calf genome and is derived from the DNA of 1.710 g/cm3 seems to be structurally related to the foregoing fragment since it shows a similar Hind II and Bsu cleavage pattern. It alternates with a 1550-nucleotide-pairs-long EcoRI fragment. In another series of experiments total calf DNA was separated into main-band and satellite fractions by density-gradient centrifugation and chromatography in the presence of a base-specific dye. Purified fractions were characterized by analytical ultracentrifugation and by Hind II and EcorRI digestions. From the cleavage patterns of purified fractions an assignment of the bands found with total calf DNA to satellite fractions was possible. Most fragments were derived from the components of density 1.709 and 1.710 g/cm3. The 1.714-g/cm3 satellite was cleaved into a 1300-nucleotide-pairs-ling EcorRI fragment and two Hind II fragments of 1100 and 180 nucleotide pairs. The satellites of 1.723 g/cm3 and 1.705 g/cm3 were not cleaved by either Hind II or EcoRI DNAase. On digestion of main band DNA with Bsu a 160-nucleotide-pairs-long fragment was obtained which was also observed, at a frequency of about 160000, in the Bsu digest of EcoRI fractions from total calf DNA.
Eur J Biochem 1975 Sep 01
PMID:Investigation of the repetitive sequences in calf DNA by cleavage with restriction nucleases. 80 85

Restriction nucleases from Escherichia coli carrying the resistance transfer factor RI, from Hemophilus influenzae, Hemophilus parainfluenzae, and Bacillus subtilis were used for the generation of specific DNA fragments from lambdadv plasmids. Cleavage maps were established for various plasmids containing different but overlapping parts of bacteriophage lambdaDNA by analysis of digestion patterns obtained in polyacrylamide gel electrophoresis. The correlation between the plasmid cleavage maps and the genetic map of lambda was based upon the location of the EcoRI cleavage site at 81.3% of lambda fractional length and the position of promoters PL and PR on the plasmid DNA fragments.
Eur J Biochem 1975 Sep 15
PMID:Mapping of cleavage sites for restriction endonucleases in lambdadv plasmids. 80 88

Numerous fungal hyphae resembling those of a phycomycete were found in thrombi, vessel walls, and areas of inflammatory cell infiltration within a large focus of necrosis in the brainstem of a 2-year-old heifer. Haemophilus somnus and Pasteurella multocida were isolated from the same lesion. Antemortem hyperglycemia was also demonstrated.
J Am Vet Med Assoc 1977 Sep 01
PMID:Phycomycosis associated with encephalitis caused by Haemophilus somnus in a heifer. 90 85

Aminopterin- or trimethoprin-resistant thymidine-requiring strains of Haemophilus influenzae produce minicells, and the ratio of minicells to cells increases during the stationary phase of growth. Strain LB11, isolated after mutagenesis of a thymidine-requiring strain (Rd thd), produces more minicells than the parent strain. The mutations involved in high frequency minicell production have been transferred into the wild type (strain Rd) by transformation. The thymidine requirement in the resulting strain, MCl, is essential for minicell production, since spontaneous revertants of MCl to prototrophy do not produce minicells. The ratio of minicells to cells was increased more than 10(3)-fold by differential centrifugation. The minicells contain little or no deoxyribonucleic acid (DNA). Phage HPlcl apparently cannot attach to minicells. Competent cells of LB11 and its thymidine-requiring parent strain produce defective phage as a result of exposure to transforming DNA, whereas only LB11 produces many defective phage in response to the competence regime alone. Competent HP1c1 and S2 lysogens of MC1 and Rd thd are also superinducible by transforming DNA, but competent LB11 lysogens produced about the same amount of HP1c1 or S2 phage with or without exposure to transforming DNA possibly because of competition between the induced defective phage and Hp1c1 or S2 phage.
J Bacteriol 1975 Sep
PMID:Minicell production and bacteriophage superinducibility of thymidine-requiring strains of Haemophilus influenzae. 108 Apr 86


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