Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We previously cloned and sequenced nqr operon encoding the Na+-translocating NADH-quinone reductase (NQR) from the marine bacterium Vibrio alginolyticus. A gene cluster very similar to nqr operon was found to exist in the genome of Haemophilus influenzae Rd. We examined the membrane fraction from H. influenzae, and the respiratory chain of H. influenzae was found to contain a Na+-dependent NQR that is essentially identical to those found in the marine V. alginolyticus. These results indicate that quite similar to the salt-loving marine bacteria, the blood-loving H. influenzae has a redox-driven Na+ pump and utilizes Na+ circulation for energy coupling.
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PMID:Existence of Na+-translocating NADH-quinone reductase in Haemophilus influenzae. 860 49

To examine the distribution of the Na(+)-translocating NADH-quinone reductase (Na(+)-NQR) among marine bacteria, we developed a simple screening method for the detection of this enzyme. By reference to the homologous sequences of the Na(+)-NQR operons from Vibrio alginolyticus and Haemophilus influenzae, a pair of primers was designed for amplification of a part of the sixth ORF (nqr6) of the Na(+)-NQR operon. When PCR was performed using genomic DNA from 13 marine bacteria, a 0.9-kbp fragment corresponding to nqr6 was amplified in 10 strains. Although there were three PCR-negative strains phylogenetically, based on the sequence of the 16S rRNA, these were placed far from the PCR-positive strains. No product was observed in the case of nonmarine bacteria. The nucleotide and predicted amino acid sequences of nqr6 were highly conserved among the PCR-positive marine bacteria. A phylogenetic analysis of marine bacteria, based on nqr6 sequencing, was performed.
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PMID:Detection of the Na(+)-translocating NADH-quinone reductase in marine bacteria using a PCR technique. 1077 68