Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Twenty-two Haemophilus cultures of types prevalent in swine and of different geographic origins were subjected to biochemical and cultural examinations. Three subgroups were identified: One was unrease-positive, produced porphyrin from delta-aminolevulinic acid, and grew on infusion mediums supplemented only with V factor; the 2nd was unrease-negative, porphyrin-positive, and grew only on serum-enriched mediums with added V factor; and the 3rd was unrease-negative, porphyrin-negative, and grew only on serum-enriched mediums with added V and X factors. The groups generally corresponded to Haemophilus parahaemolyticus, Haemophilus parasuis, and Haemophilus suis, respectively. By means of the unrease and porphyrin tests, it was possible to assign, presumptively, porcine haemophilus cultures to 1 of the 3 species. Other tests, such as beta-galactosidase, hemolysis, and fermentation of carbohydrates were of secondary value in differentiating between these species.
 ...
PMID:Cultural and biochemical criteria for the identification of haemophilus spp from swine. 18 48

White, David C. (The Rockefeller Institute, New York, N.Y.) and S. Granick. Hemin biosynthesis in Haemophilus. J. Bacteriol. 85:842-850. 1963.-Hemin-independent Haemophilus species have been shown to form hemin by the classical hemin biosynthetic pathway. Three distinct species of Haemophilus [H. influenzae, H. aegyptius, and H. canis (H. haemoglobinophilus)] all lost the enzymatic capacities to convert delta-aminolevulinic acid to protoporphyrin, which accounts for their dependence on hemin for growth. The strain of H. aegyptus tested cannot form hemin from protoporphyrin, can be transformed with deoxyribonucleic acid (DNA) from H. influenzae, and the resultant progeny have the enzymatic activity to convert protoporphyrin to hemin. Attempts to transform these species to hemin independence with DNA from hemin-independent H. parainfluenzae are unsuccessful under conditions where streptomycin resistance is readily transformed.
 ...
PMID:HEMIN BIOSYNTHESIS IN HAEMOPHILUS. 1404 53

Biberstein, Ernst L. (University of California, Davis), Patricia D. Mini, and Marjorie G. Gills. Action of Haemophilus cultures on delta-aminolevulinic acid. J. Bacteriol. 86:814-819. 1963.-Utilization of delta-aminolevulinic acid (ALA) by strains of Haemophilus recovered from various sources was investigated. With the 37 cultures studied, there was perfect correlation between absence of hemin requirement and ability to convert ALA to porphyrin. A total of 29 strains, including representatives of H. parainfluenzae, H. parahaemolyticus, H. "parasuis," and H. gallinarum, fell into this group. The remaining eight isolates, which were incapable of porphyrin synthesis from ALA, were strains of H. influenzae, H. suis, and a Haemophilus culture of uncertain classification obtained from a splenic abscess in a deer. In the active preparations, the products of synthesis included a mixture of porphyrins, porphobilinogen (PBG), and a pigment which absorbed light strongly at 470 to 480 mmu. Paper chromatographic studies of fractions of supernatants and cells revealed the presence of porphyrins having R(F) values similar to those of uro- and protoporphyrins, as well as some intermediate rates of migration probably representing coproporphyrins. Porphyrins were found both intra- and extracellularly, and PBG and the pigment absorbing at 470 to 480 mmu were confined to the supernatant.
 ...
PMID:ACTION OF HAEMOPHILUS CULTURES ON DELTA-AMINOLEVULINIC ACID. 1406 79