Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZ alpha-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.
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PMID:Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes. 762 22

Haemophilus influenzae is a fastidious Gram-negative coccobacilli that is a common commensal in the human upper respiratory tract. However, certain strains of this bacterium, including those considered to be nontypeable (NTHi), can cause human diseases ranging from otitis media to meningitis. Although naturally competent, NTHi take up plasmids by transformation very inefficiently, if at all. Many clinical isolates have also proven refractory to the introduction of currently available shuttle vectors via electroporation. Further, it has been difficult to determine protein expression from these vectors, unless specific antisera has been raised or a phenotype conferred. To address these problems, we have designed and constructed a set of broad host range vectors that are transferable via intergeneric conjugation with an Escherichia coli strain carrying chromosomally-encoded transfer functions. These vectors provide a site for cloning promoter::MCS regions and carry genes encoding resistance to one of two different antibiotics. This conjugal system allows the expression of marker genes in NTHi strains, enabling researchers to track the microbe's progress either in vivo using the infant rat model of infection, or in vitro through invasions of human tissue culture cell lines.
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PMID:Design and construction of a Haemophilus influenzae conjugal expression system. 1175 Jan 31