Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This study was designed to clone the aroA gene (encoding 5-enolpyruvylshikimate-3-phosphate synthase [EPSPS]) from Haemophilus influenzae using a mixed-primer polymerase chain reaction (PCR). The mixed primers were based on a back translation of previously reported blocks of amino acid homology between different cloned EPSPS proteins. The PCR-produced probe from H. influenzae DNA was used to identify a recombinant lambda phage from which the rest of H. influenzae aroA was subcloned and sequenced. The sequence of H. influenzae aroA is highly homologous to all other aroA genes sequenced to date. The upstream sequence of aroA, when translated, results in a protein with strong homology to the purN gene product, glycinamide ribonucleotide transformylase. The stop codon of the putative H. influenzae purN gene overlaps the start codon for H. influenzae aroA, suggesting that the two genes may form an operon and that they may be translationally coupled.
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PMID:Cloning and sequencing of the Haemophilus influenzae aroA gene. 833 55

Haemophilus haemolyticus has been recently discovered to have the potential to cause invasive disease. It is closely related to nontypeable Haemophilus influenzae (NT H. influenzae). NT H. influenzae and H. haemolyticus are often misidentified because none of the existing tests targeting the known phenotypes of H. haemolyticus are able to specifically identify H. haemolyticus Through comparative genomic analysis of H. haemolyticus and NT H. influenzae, we identified genes unique to H. haemolyticus that can be used as targets for the identification of H. haemolyticus A real-time PCR targeting purT (encoding phosphoribosylglycinamide formyltransferase 2 in the purine synthesis pathway) was developed and evaluated. The lower limit of detection was 40 genomes/PCR; the sensitivity and specificity in detecting H. haemolyticus were 98.9% and 97%, respectively. To improve the discrimination of H. haemolyticus and NT H. influenzae, a testing scheme combining two targets (H. haemolyticus purT and H. influenzae hpd, encoding protein D lipoprotein) was also evaluated and showed 96.7% sensitivity and 98.2% specificity for the identification of H. haemolyticus and 92.8% sensitivity and 100% specificity for the identification of H. influenzae, respectively. The dual-target testing scheme can be used for the diagnosis and surveillance of infection and disease caused by H. haemolyticus and NT H. influenzae.
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PMID:Comparative Genomic Analysis of Haemophilus haemolyticus and Nontypeable Haemophilus influenzae and a New Testing Scheme for Their Discrimination. 2770 39