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Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The paper reports on the drawing up and experimentation of a kit of media for identification in liquid media and biotyping of H. influenza and H. parainfluenza. The kit is made up of 14 components, out of which the following were prepared: basic broth, X V broth, V broth, X broth, red-phenol broth, and XV factors + ribose, xylose and saccharose, urea substrate. Moeller medium with XV, with and without ornithine, covered with paraffin oil after impregnation. In the XV broth and the broth with red phenol the XV factors and saccharose, the bands for indole and H2S were put after impregnation with bacterial culture. On these media, 175 strains of Haemophilus were identified and biotyped. 109 of them were H. influenzae and 66 H. parainfluenzae, when satellitism was used. Identification in liquid media showed that 5.5% of the H. influenza strains were H. parainfluenza and 10.6% of the H. parainfluenza strains were H. influenza. Finally, 110 strains were H. influenzae and 65 H. parainfluenzae. The components of the kit permitting identification and biotyping of H. influenzae and H. parainfluenzae are: basic broth with red phenol, X broth, XV factors supplement, V supplement, mono- and bipotassium phosphate solution, 20% urea solution, 20% saccharose, 20% ornithine, Moeller medium, bands for indole and paraffine oil.
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PMID:[The development of and experimentation with a kit for the identification and rapid biotyping of H. influenzae and H. parainfluenzae]. 180 92

The 2-hr quadFERM+ kit (qF) (Analytab Products, Plainview, NY) was compared with conventional tube tests for the identification of the HACEK bacteria (Haemophilus aphrophilus, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, Kingella kingae), other Haemophilus and Kingella spp., Capnocytophaga, Corynbacterium, and Moraxella spp. Test results were identical for 296 comparisons with 74 isolates (74%). In the remaining 104 comparisons for 26 isolates, 50 reactions were identical, and qF produced a positive result in 46 of 54 discrepancies.
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PMID:Application of quadFERM+ for the identification of fastidious gram-positive and gram-negative bacilli. 187 78

Several rapid method kits (one to four hours) have become available for the identification of Haemophilus and related genera. Two kits (the "Rapid NH" system and the "RIM Haemophilus" system), which include the identification and biotyping of H influenzae, were investigated for the rapid identification and biotyping of 193 isolates of H influenzae and the results compared with those obtained by more standard overnight methods. The kits were convenient to use and gave reliable and rapid speciation of all isolates. Both test systems were unreliable for biotyping: 42 isolates were wrongly biotyped by the RIM kit and 40 isolates wrongly biotyped by the rapid NH kit. It is concluded that the test kits may be useful for the rapid identification of H influenzae but that they are not reliable for the biotyping of this species.
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PMID:Evaluation of two rapid methods for identifying and biotyping Haemophilus influenzae. 201 34

The ability of a commercial latex kit to detect pneumococcal antigen in 50 CSF samples, 150 ear swabs and 80 sputum samples was investigated. Results were compared with microscopic and culture findings. 18/19 culture-positive specimens were latex-positive. 7 latex-positive specimens yielded organisms other than S. pneumoniae which agglutinated the latex, 4 Klebsiella spp, 2 Staphylococcus aureus and 1 Haemophilus influenzae. For 11 other specimens latex agglutination was positive and culture negative. S. pneumoniae was recovered from the blood of 1 of these patients and, in 2 further cases, microscopy showed poorly stained organisms which had some resemblance to S. pneumoniae. For 10 different strains of S. pneumoniae suspensions containing between 10(4) and 10(7) organisms per ml were required to agglutinate the latex.
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PMID:Evaluation of a latex kit reagent for the detection and identification of pneumococci. 317 73

A commercial latex agglutination (LA) kit (Wellcogen, Wellcome Diagnostics) used to detect bacterial polysaccharide antigens (Haemophilus influenzae type b and Streptococcus pneumoniae) was compared with a modified counterimmunoelectrophoresis technique and blood culture for etiologic diagnosis of presumptive bacterial pneumonia requiring hospitalization in 60 infants and children. Serum, urine and either sputum or nasopharyngeal secretions were collected during the first 5 days of therapy for antigen detection. Blood culture was positive in 6 of 52 (11.5%) of cases. Antigens were detected by counterimmunoelectrophoresis and/or LA in 13 of 60 (21.7%) serum samples, 2 of 16 (12.5%) unconcentrated urine samples, 19 of 42 (45.2%) urine samples concentrated 25-fold and 21 of 45 (46.7%) sputum or nasopharyngeal secretions. Antibiotic treatment for 5 days did not affect the antigen detection rate. Counter-immunoelectrophoresis was more sensitive than LA in serum and urine but not in sputum. However, because false positive reactions were frequently obtained with LA on nasopharyngeal secretions of an age-matched control group, this test appears unreliable.
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PMID:Counterimmunoelectrophoresis and latex particle agglutination in the etiologic diagnosis of presumed bacterial pneumonia in pediatric patients. 326 6

The validity of commercial latex agglutination kits for detection of Haemophilus influenzae type b and Streptococcus pneumoniae antigens in serum and urine specimens was studied. We tested serum and urine specimens from 44 patients with bacteremic pneumonia (23 S. pneumoniae, 13 H. influenzae type b, 11 other) with commercial latex agglutination kits (Directigen, Bactigen) for S. pneumoniae and H. influenzae type b antigens. All specimen samples were randomized and read blindly by two readers. Interreader reproducibility was 100%. The sensitivity and specificity of both kits for H. influenzae type b antigens in serum and urine were greater than 90%. None of the 24 urine samples from S. pneumoniae bacteremic patients were positive by either kit, although 6 ng of type 3 polysaccharide could be detected in spiked urine. Sensitivity for S. pneumoniae antigens in serum was 27% for Directigen and 38% for Bactigen. Specificity for S. pneumoniae antigens in serum was 95% for Directigen and 74% for Bactigen. The results suggest that the kits are useful in diagnosing H. influenzae type b pneumonia. However, the commercially available S. pneumoniae reagents tested appear to have limited utility for diagnosing S. pneumoniae pneumonia because both kits lack sensitivity and Bactigen lacks specificity, as well.
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PMID:Commercial latex agglutination tests for detection of Haemophilus influenzae type b and Streptococcus pneumoniae antigens in patients with bacteremic pneumonia. 349 43

The capsular polymer (CP) of Haemophilus pleuropneumoniae serotype 5 was purified, and its chemical composition was analyzed. Radioimmunoassay experiments showed that the maximum amount of CP could be obtained from broth cultures of bacteria in the late stationary phase, rather than from bacteria washed off agar plates. The CP was precipitated from culture supernatant with 5 mM hexadecyltrimethylammonium bromide (Cetavlon) and solubilized with 0.4 M NaCl. Ninety percent of the CP in the culture supernatant was precipitated with Cetavlon, although some material remained insoluble after NaCl extraction. The CP was further purified by phenol extraction, ultracentrifugation, and Sepharose CL-4B gel filtration. The Kav of the CP from Sepharose CL-4B chromatography was 0.33. The CP preparation contained 85% hexosamine, 12% hexose, 3% phosphate, 0.17% protein, 0.20% nucleic acid, and 0.01% endotoxin. Thin-layer chromatography, an amino acid analyzer, and a glucose oxidase colorimetric kit were used to identify the sugar components of the hydrolyzed CP as glucosamine and glucose. Analysis of the native CP by 13C nuclear magnetic resonance indicated that amino, N-acetyl, and carboxyl groups were present and that the CP was a disaccharide.
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PMID:Purification and partial characterization of the capsular polymer of Haemophilus pleuropneumoniae serotype 5. 359 1

Twenty samples of CSF, 11 from proven meningitis with positive culture and nine negative controls were examined. Ten cases (91%) of meningitis, six caused by Haemophilus influenzae, three by Streptococcus pneumoniae and one by Neisseria meningitidis have been diagnosed by the coagglutination method. Using the Phadebact CSF kit, the test was negative, in one (9%) meningitis case, which was caused by Str. pneumoniae. Subsequently all cases of meningitis were confirmed by isolation of the organisms in CSF culture. None of the nine control CSF gave a positive reaction with coagglutination test or positive culture. The coagglutination test is simple, rapid and requires no special equipment.
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PMID:Rapid laboratory diagnosis of bacterial meningitis employing coagglutination test. 362 5

Of 7,871 blood cultures from hospital patients, 22 yielded growth of Streptococcus pneumoniae or Haemophilus influenzae type b. The identities of 19 (86%) of these 22 strains could be verified after 18 to 24 h of incubation by application of the Directigen meningitis test kit to the unheated, uncentrifuged supernatant from the cultures; thus, the turnaround time for these cultures was halved. Growth in 16 (72%) of the Directigen-positive cultures was detected by visual inspection, and that in 3 (14%) of the cultures was detected by acridine orange staining. Growth in the three remaining bottles (14%) was detected by blind subculturing after 18 to 24 h or incubation and, therefore, was delayed by 24 h. The systematic application the acridine orange stain was helpful in 40 (44%) of 91 cases for which macroscopic inspection failed to reveal growth after 24 h of incubation.
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PMID:Use of Directigen and acridine orange for rapid identification of human blood culture isolates. 633 34

Authors have searched for Haemophilus influenzae by culture and by detection of capsular antigens in CSF of 1.700 bacterial meningitis in Dakar. Culture has been positive in 71.9%, counter-immunoelectrophoresis in 94.9% and latex (slidex meningitis kit) in 91.2% of cases. Diagnosis results are improved of 39% comparatively with bacteriologic culture. False positive results caused by antigenic cross-reactions have been observed only in 1.3% of others bacterial meningitis. Latex-test is allowing a diagnosis in three minutes and a quantitative determination of antigens (useful for pronostic).
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PMID:[Latex agglutination test and counterimmunoelectrophoresis in the diagnosis of Haemophilus influenzae meningitis]. 634 52


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