Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0348321 (
Haemophilus
)
15,372
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Over the past four decades, the incidence of meningitis caused by
Haemophilus
influenzae
in children has decreased due to widespread vaccination against
H. influenzae
type b (Hib). The incidence of invasive diseases due to
H. influenzae
types not included in the vaccines, however, has increased. At present, there are a limited number of diagnostics available to detect non-type b
H. influenzae
. To address this issue, we developed a rapid, simple, and cost-effective method for detecting serotypes of
H. influenzae.
We designed
LAMP
primer sets based on published sequences for
H. influenzae
capsular types a, c, d, e, and f. The assay was evaluated to determine test reactivity, specificity, and sensitivity. To support its use in patients with suspected meningitis, we evaluated the detection limit of the non-Hib serotype specific
LAMP
assay using bacterial genomic DNA-spiked cerebrospinal fluid (CSF) specimens. The reactivity and specificity of the
LAMP
assays were confirmed using six serotypes and non-typeable
H. influenzae
strains, plus eight strains of other
Haemophilus
species and non-
Haemophilus
genera. The detection limits of the
LAMP
assay for capsular types a, c, d, e, and f were 10
2
, 10
2
, 10
2
, 10
3
, and 10 copies per reaction, while those of the PCR assay were 10
4
, 10
4
, 10
3
, 10
3
, and 10
4
genome copies per reaction, respectively. Using DNA-spiked CSF specimens, the detection limit of the
LAMP
assay was equivalent to that using purified DNA as the template. However, the detection limit of the PCR was reduced from 10
3
to 10
4
genome copies per reaction for serotype d and from 10
3
to 10
5
genome copies per reaction for serotype e. To the best of our knowledge, this is the first report of a serotype-specific identification assay for
H. influenzae
using the
LAMP
method. Our results suggest the potential of
LAMP
methods for patients with suspected meningitis in resource-limited laboratories or public health surveillance systems.
...
PMID:Molecular Serotype-Specific Identification of Non-type b
Haemophilus influenzae
by Loop-Mediated Isothermal Amplification. 2904 66
