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Query: UMLS:C0348321 (Haemophilus)
 15,372 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In a haemolytic plaque assay staphylococcal strain Cowan 1 was shown to induce polyclonal antibody secretion in human blood lymphocytes, whereas Haemophilus influenzae and Escherichia coli gave low responses. Diplococcus pneumoniae and haemolytic streptococci generally did not activate blood cells. All five bacteria could activate spleen, tonsil and adenoid cells both to polyclonal Ig secretion and increased DNA synthesis. Thus blood cell reactivity does not necessarily reflect the response pattern in other lymphatic organs. The adenoid was shown to contain lymphocytes more responsive to bacteria normally residing in nasopharynx than cells residing in other lymphatic organs. On the other hand, spleen and mesenteric lymph node contain a subpopulation of cells highly responsive to bacteria such as Escherichia coli normally residing in the bowel. Therefore, we conclude that there exists a functional compartmentalization of lymphocytes in distinct secondary lymphoid organs.
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PMID:The use of bacteria for the functional characterization of human lymphocyte subpopulations in various lymphoid organs. 3 77

The regulation of age-related antibody response to Haemophilus influenzae type b polysaccharide (HITB-PS) was studied by measuring the splenic plaque forming cells (PFC) following immunization with this capsular polysaccharide. The magnitude of PFC response to HITB-PS was found to be dose-related, enhanced by Freund's complete adjuvant and influenced by the genetic strain of mice. Priming with a low dose of HITB-PS did not induce a state of immunological unresponsiveness. Treatment with antilymphocyte serum significantly increased the PFC response to HITB-PS. Athymic nude mice showed an enhanced ability to induce both IgG and IgA-PFC responses as well as a significant increase in the biosynthesis of protein and mitogenicity in spleen cells. These findings suggest that the immune response to HITB-PS is regulated by the suppressor T cell. The magnitude of the IgM-PFC response induced by HITB-PS in mice increased gradually from two weeks of age and reached a plateau at 8 weeks. Treatment with fetuin resulted in the inhibition of direct IgM and IgG-PFC responses to HITB-PS; the suppressive effect on the immune response was more profound and lasting in young than in adult mice.
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PMID:The regulation of the immune response of mice to Haemophilus influenzae type b capsular polysaccharide. 7 78

A small-plaque polyoma virus, MPC-1, was isolated from a mouse plasmacytoma. The DNA of this polyoma virus was cleaved with a restriction enzyme from Haemophilus influenzae (Hin d), and the molecular weights of the limit products were analyzed by electrophoresis and electron microscopy. The fragments produced by this enzyme have been ordered by analysis of partial digest products. A physical map of the polyoma virus genome was then constructed.
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PMID:Studies of polyoma virus DNA: cleavage map of the polyoma virus genome. 16 43

The initial phases of plaque development on nonretentive tooth surfaces were studied bacteriologically in Macaca irus monkeys fed by stomach tube and provided with various oral supplements. Except for the oral implantation of Streptococcus mutans in some of the animals, the oral flora was not changed prior to the studies. Dental plaque was allowed to develop on initially cleaned tooth surfaces for 3 to 5 h. Plaque samples were collected and cultured on a number of selective and nonselective agar media, and several hundred isolates from each sample were isolated and identified. The numerically predominant organisms in initial plaque were S. mutans, Streptococcus sanguis, and Actinomyces viscosus. Additional organisms regularly found, but usually in smaller numbers, were Streptococcus mitior and a group of fastidious gram-negative rods including Haemophilus species, Eikenella corrodens, and Actinobacillus actinomycetem-comitans. The colonization of S. mutans was dependent on sucrose and occurred at the expense of S. sanguis. In these experiments S. mutans accounted for 25 to 65% of the primary plaque formers. All other species encountered colonized the teeth irrespective of the diet. It is postulated that the early sucrose-dependent establishment of S. mutans directly on the enamel pellicle plays a key role in the development of a cariogenic plaque.
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PMID:Initial colonization of teeth in monkeys as related to diet. 82 62

The mean concentration of haemophili in 31 specimens of plaque was 1.23 times 10(6) per milligram or approximately 4.4% of the viable bacteria present. Occurrence of different species was similar to saliva with Haemophilus parainfluenzae constituting 87.8%. Most haemophili produced neuraminidase and appeared to be primarily responsible for the small amounts of this enzyme in plaque.
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PMID:Occurrence of haemophili in dental plaque and their association with neuraminidase activity. 105 57

Evidence for a possible role played by oral haemophili in the development of dental plaque was sought by studying the occurrence of these bacteria in early dental plaque of smooth surfaces and occlusal fissures in six dental students. The mean number of haemophili per 10(3) anaerobes in early smooth surface plaque (18 h) and fissure plaque (7 d) was 95 and 22 respectively. Examination of 988 Haemophilus isolates revealed that H. parainfluenzae was the only species in samples of fissure plaque, whereas some samples from smooth surfaces, in addition to the predominating and ubiquitous H. parainfluenzae, yielded growth of the two species H. segnis and H. aphrophilus. It is concluded that haemophili are among the primary colonizers of smooth surfaces of teeth.
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PMID:Haemophili in developing dental plaque. 106 88

During bacteriophage studies on Haemophilus influenzer, it was observed that encapsulated type b and unencapsulated Rb strains released a bactericidal substance acitve against types a, c, d, e, and f H. influenzae, non-typable H. influenzae strains, other Haemophilus species, and certain members of the Enterobacteriaceae. The bactericidal activity was assayed by a plaque test utilizing an Rd strain as an indicator lawn and was also demonstrated in mixed broth cultures of a producer strain and an indicator strain. Immediately lysis of sensitive bacteria by the factor was not evident. The factor is sensitive to trypsin but resistant to deoxyribonuclease, treatment with 2-mercaptoethanol, lipase, alpha-amylase, and heating in a 100 degrees C water bath for 20 min. The activity is not dependent upon increased Ca2+ or Mg2+ concentration as is necessary for HP1C1 and S2 phage propagation. The bactericidal factor is not pelleted by high-speed centrifugation at 150,000 X g for 6 h. Treatment with ultraviolet light or mitomycin C does not result in observable phage, phage-like particles, or increased bactericidal activity. T-HE BACTERICIDAL FACTOR IS NOT A TYPICAL SMALL MOLECULAR WEIGHT "COLICIN-LIKE" BACTERiocin in that it is not inducible, has a wider range of activity, and does not kill by "single-hit" kinetics. On preliminary characterization, it is a thermostable protein toxic to certain bacterial strains.
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PMID:Bactericidal substance produced by Haemophilus influenzae b. 108 28

Recent advances in the development of conjugate polysaccharide vaccines for human use have stimulated interest in the use of assays detecting antibody-secreting cells (AbSC) with specificity for bacterial antigens. Here we present improved haemolytic plaque-forming cell (PFC) assays detecting AbSC with specificity for tetanus and diphtheria toxoid as well as for Haemophilus influenzae type b and pneumococcal capsular polysaccharides. These assays were found to be less time consuming, more economical and yielded 1.9-3.4-fold higher plaque numbers than traditional Jerne-type PFC assays. In the case of anti-polysaccharide AbSC of the IgG isotype, the increase was as high as 7.4-11.8 times. Evidence is presented that the pronounced improvement in the detection of the latter is due to the presence of aggregating anti-IgG antibody from the beginning of the assay. It is proposed that in the case of low affinity of anti-polysaccharide antibodies aggregation of secreted monomeric antibody (IgG) is critical for plaque formation and increases the avidity of binding to target cells.
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PMID:An improved haemolytic plaque assay for the detection of cells secreting antibody to bacterial antigens. 173 77

The prevalence and distribution of Haemophilus actinomycetemcomitans (H.a.) were studied in 3292 specimens of subgingival plaque on the four subgingival aspects of all teeth of the dental arch, 150 specimens from the mucosal surface (tongue and cheek) and 30 saliva specimens in 30 subjects. The sample population of 30 subjects was subdivided into three groups: 10 normal subjects, 10 subjects with localised juvenile periodontitis (SLJP) and 10 subjects with adult chronic periodontitis (SACP). The prevalences of H.a. in subgingival areas of each group mentioned were 30%, 90% and 60% respectively. Scores for prevalence obtained with other types of specimens proved to be lower except for saliva specimens which appear to be a less representative marker of subgingival prevalence of H.a.. Histograms for the distribution of H.a. revealed a predominance of this microorganism on the proximal surface of molar teeth in the three groups of patients. Only the SLJP also exhibited a high prevalence on the proximal aspect of the incisor teeth. The wide distribution of H.a. in all of the clinical groups studied suggests that this bacterium is not a good marker of periodontal disease and that it is necessary to define the most characteristic phenotypes and genotypes.
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PMID:[The distribution and prevalence of Haemophilus actinomycetemcomitans in the oral cavity]. 193 44

Serum IgG, IgM, and IgA antibody levels to extracts of rat dental plaque and five oral bacteria (Haemophilus actinomycetemcomitans Y-4, Bacteroides gingivalis 381, Bact. intermedius ATCC 25261, Capnocytophaga sp. M-12, Eikenella corrodens ODU) were determined by ELISA. In addition, the presence of rat dental plaque and oral bacterial components in the inflamed gingival tissue was studied using immunofluorescence techniques. Serum and gingival tissue samples were obtained from ODU plaque-susceptible and plaque-resistant rats. In several susceptible rats, IgG, IgM, and IgA antibodies against dental plaque and oral bacteria were detected. There was a correlation between the levels of IgG antibody to dental plaque and pocket probing depth, but not between pocket probing depth and the levels of IgM and IgA. Furthermore, components of rat dental plaque and oral bacteria were detected in the inflamed gingival tissue.
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PMID:Humoral immune responses in experimental gingivitis in rats. 208 26


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