Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0345904 (
liver cancer
)
15,188
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Nuclear-enriched RNA-binding proteins (RBPs) are mainly involved in transcriptional regulation, which is a critical checkpoint to tune gene diversity and expression levels. We analyzed nuclear RBPs in human
HCC
tissues and matched normal control tissues. Based on the gene expression levels,
PTBP3
was identified as top-ranked in the nuclei of
HCC
cells.
HCC
cell lines then were transfected with siRNAs or lentiviral vectors.
PTBP3
promoted
HCC
cell proliferation and metastasis both in vitro and in vivo. RNA immunoprecipitation (RIP), fluorescence in situ hybridization (FISH) and qRT-PCR assays verified that
PTBP3
protein recruited abundant lnc-NEAT1 splicing variants (NEAT1_1 and NEAT1_2) and pre-miR-612 (precursor of miR-612) in the nucleus. NEAT1_1, NEAT1_2 and miR-612 expression levels were determined by
PTBP3
. Correlational analyses revealed that
PTBP3
was positively correlated with NEAT1, but it was inversely correlated with miR-612 in
HCC
. The P53/CCND1 and AKT2/EMT pathways were determined by NEAT1 and miR-612 respectively in
HCC
. The
PTBP3
high
and NEAT1
high
/miR-612
low
patients had a shorter overall survival. Therefore, nuclear-enriched RBP,
PTBP3
, promotes
HCC
cell malignant growth and metastasis by regulating the balance of splicing variants (NEAT1_1, NEAT1_2 and miR-612) in
HCC
.
...
PMID:PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612. 3006 40
