Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: UMLS:C0345904 (
liver cancer
)
15,188
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The role and clinical implication of the
WWP2
E3 ubiquitin ligase in
liver cancer
are poorly understood. In the current study, we investigated the expression level of
WWP2
and its functions in cell adhesion, invasion, and migration in
liver cancer
. We used real-time PCR to detect the expression of
WWP2
in
liver cancer
and adjacent samples from the People's Hospital of Lishui and also analyzed The Cancer Genome Atlas (TCGA) RNA-seq data by bioinformatics. Migration and invasion were detected by transwell analysis. We detected a strong
WWP2
expression in tumor tissues of the People's Hospital of Lishui, and the survival rate was significantly higher in patients with lower
WWP2
-expressing tumors.
WWP2
small hairpin RNA (shRNA) lentivirus stably infected cells (shWWP2), Huh7, showed slower growth speed compared with scramble control-infected cells in a xenograft mouse model. Knockdown of
WWP2
Huh7 and BEL-7404 cells demonstrated a reduction in adhesion, invasion, and migration. Gene set enrichment analysis (GSEA) showed that
WWP2
is positively correlated to cancer-related pathways including the chemokine signaling pathway.
WWP2
also regulated MMP-9, caspase-9, CXCR3, and CCR5 expression in
liver cancer
cells. In addition, knockdown of CXCR3 and CCR5 significantly inhibited cell proliferation, adhesion, invasion, and migration in Huh7 and BEL-7404 cells. Our data suggest that targeting of
WWP2
may be a therapeutic strategy for
liver cancer
treatment.
...
PMID:Silencing of WWP2 inhibits adhesion, invasion, and migration in liver cancer cells. 2666 6
Alternative splicing within a gene can create different versions of an mRNA, called isoforms. CFIm, composed of a small subunit CFIm25 and two large subunits CFIm68 and CFIm59 (also known as CPSF7), has been proposed as an enhancer-dependent activator of mRNA 3' processing. In this study, we investigated the role of CPSF7 in hepatocellular carcinoma. Experimental evidence suggests that the expression level of CPSF7 is higher in
liver cancer
cells and tissues than in non-tumor hepatic cells and tissues. Furthermore, knockdown of CPSF7 effectively suppressed cell proliferation, migration and colony formation in
liver cancer
cells by inhibiting PTEN/AKT signaling. CPSF7 promoted
WWP2
-FL due to the presence of PTEN ubiquitination sites in this longer transcript. Taken together, we identified that CPSF7 regulates
liver cancer
growth by targeting
WWP2
-FL that in turn regulates AKT activation in a PTEN-dependent manner.
...
PMID:CPSF7 regulates liver cancer growth and metastasis by facilitating WWP2-FL and targeting the WWP2/PTEN/AKT signaling pathway. 3183 82
