Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0345904 (liver cancer)
 15,188 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Sequence-specific resonance assignments provide the basis for interpreting multidimensional NMR spectra and for determining 3D structures of proteins from these data. We have developed an improved strategy for determining these sequence-specific NMR assignments in small proteins and applied this method in determining proton and nitrogen resonance assignments for an 8.2-kDa engineered domain (the Z-domain) of the cell wall protein A of Staphylococcus aureus. First, HCCNH-TOCSY [Lyons, B. A. & Montelione, G.T. (1993) J. Magn. Reson. 101B, 206] data were used together with 2D 2QF-COSY, TOCSY, and 15N-HSQC data to identify amino acid spin systems. Most asparagine and glutamine spin systems were also identified uniquely from these triple-resonance data. Next, complementary HCC(CO)-NH-TOCSY [Montelione, G. T., et al. (1992) J. Am. Chem. Soc. 114, 10975] data were used to identify sequential connections from the aliphatic H alpha, H beta, H gamma, H delta, and H epsilon resonances of residue i to the amide and nitrogen resonances of residue i + 1. By combined analysis of HCCNH-TOCSY and HCC(CO)NH-TOCSY spectra we have determined most of the proton and nitrogen resonance assignments for the Z-domain. This represents the first example of the use of this triple-resonance technique to determine extensive resonance assignments in a small protein.
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PMID:An improved strategy for determining resonance assignments for isotopically enriched proteins and its application to an engineered domain of staphylococcal protein A. 839 17

Investigation of surface-enhanced Raman spectra of oxyhemoglobin for 30 liver cancers and 30 normal persons based on the silver nanofilm was reported. Principal components analysis (PCA) and independent variable T test were employed to develop effective diagnostic algorithms for distinguishing liver cancer form normal. Discriminant analysis was used to evaluate the diagnostic efficiency and the result indicates that SERS spectra of the oxyhemoglobin are obviously different between normal persons and liver cancers. PCA and independent variable T test were employed to get a three-dimensional scatter plot of PC scores for the healthy and cancer groups, and it can be learned that they are distributed in separate areas. By using the method of discriminate analysis, it was fount that the diagnostic algorithm separates the two groups with sensitivity of 96.7% and diagnostic specificity of 90%, the overall diagnostic accuracy was 93.3%. By analyzing the assignations of the SERS bands, it was found that the content of asparagine, tyrosine and phenylalanine in the hemoglobin are significantly lower than healthy people. The results from this exploratory study demonstrate that SERS detection of oxyhemoglobin for liver cancers based on the method of PCA combined with independent variable T test is expected to develop into a new type of liver cancer diagnostic tool.
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PMID:[Analysis of surface enhanced Raman scattering spectra of oxyhemoglobin for liver cancer with combined multivariate statistics]. 2324 Apr 11

Liver cancer is one of leading digestive malignancies with high morbidity and mortality. There is an urgent need for the development of novel therapies for this deadly disease. It has been proven that asparagus polysaccharide, one of the most active derivates from the traditional medicine asparagus, possesses notable antitumor properties. However, little is known about the efficacy of asparagus polysaccharide as an adjuvant for liver cancer chemotherapy. Herein, we reported that asparagus polysaccharide and its embolic agent form, asparagus gum, significantly inhibited liver tumor growth with transcatheter arterial chemoembolization (TACE) therapy in an orthotopic hepatocellular carcinoma (HCC) tumor model, while significantly inhibiting angiogenesis and promoting tumor cell apoptosis. Moreover, asparagine gelatinous possessed immunomodulatory functions and showed little toxicity to the host. These results highlight the chemotherapeutic potential of asparagus polysaccharide and warrant a future focus on development as novel chemotherapeutic agent for liver cancer TACE therapy.
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PMID:Asparagus polysaccharide and gum with hepatic artery embolization induces tumor growth and inhibits angiogenesis in an orthotopic hepatocellular carcinoma model. 2560 7