Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0345904 (
liver cancer
)
15,188
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
RAD54 homolog B is a central motor protein of homologous recombination (HR), which plays an important role in the development and evolution of human cancer. Recent studies have indicated that
RAD54B
is aberrantly expressed in a variety of cancers and exhibits numerous biological functions, including participating in the repair of DNA double strand breaks. However, the expression and function of
RAD54B
in
liver cancer
have not yet been reported. The aim of the present study was to investigate the expression of
RAD54B
and elucidate its role in
liver cancer
cell lines by inhibiting
RAD54B
using a lentivirus-mediated shRNA interference system. We also assessed the effect of
RAD54B
on cell proliferation, colony formation, cell cycle distribution and cell apoptosis in BEL-7404 and SMMC-7721 cell lines using shRAD54B or shCtrl transfection. Furthermore, we analyzed the relationship between the expression of
RAD54B protein
, as measured by immunohistochemical staining, and the prognosis of patients with hepatoma. We found that
RAD54B
was highly expressed in
liver cancer
cell lines compared with the normal hepatic cell line LO2. Similarly, positive expression of
RAD54B
, which is associated with poor prognosis, was also observed in 52/83 samples of
liver cancer
tissue. Additionally,
RAD54B
downregulation significantly inhibited cell proliferation and colony formation, while also inducing G1/S cell cycle arrest and apoptosis in BEL-7404 and SMMC-7721 cells. These results indicated that
RAD54B
has oncogenic properties, and may be a potential treatment target for
liver cancer
patients.
...
PMID:Inhibition of RAD54B suppresses proliferation and promotes apoptosis in hepatoma cells. 2995 8
