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Target Concepts:
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Query: UMLS:C0344329 (
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28,634
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Amphiphysin I is an SH3 domain-containing neuronal protein, enriched in axon terminals, which was reported to act as a physiological binding partner for dynamin I in synaptic vesicle endocytosis. Rvs167 and Rvs161, the yeast homologs of
amphiphysin I
, have been implicated in endocytosis, actin function, and cell polarity. Now we have explored the possibility that
amphiphysin I
also may have a role in actin dynamics and cell polarity by testing the effect of
amphiphysin I
suppression on neurite outgrowth. Freshly plated hippocampal neurons were exposed to antisense oligonucleotides via a new delivery system based on a polycationic amphipathic polymer, PS980. Western blot analysis revealed that
amphiphysin I
levels steadily increased with neuronal differentiation, whereas in antisense-treated cultures
amphiphysin I
levels were reduced to approximately 10% of control levels at 48 hr. Concomitantly, a
collapse
of growth cones and a severe inhibition of neurite outgrowth and axon formation were observed. A similar effect was observed previously after dynamin I suppression in the same culture system (). We also have found that
amphiphysin I
and dynamin I colocalize in developing neurons at all developmental stages and that a pool of both proteins is colocalized with actin patches at the leading edge of growth cones. Our findings suggest a conserved role of the amphiphysin protein family in the dynamics of the cortical cell cytoskeleton and provide new evidence for a close functional link between
amphiphysin I
and dynamin I.
...
PMID:Amphiphysin I antisense oligonucleotides inhibit neurite outgrowth in cultured hippocampal neurons. 941 89
