Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0344329 (collapse)
 28,634 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Monensin, a carboxylic ionophore, collapse Na+ gradient across biological membranes, increases intracellular pH and affects many processes involved in transport, posttranslational modification and secretion of proteins, moreover endocytosis and degradation in lysosomes. Concerning the liver, the action of monensin in vitro or in perfused liver, showed an altered secretion of protein and altered transport of VLDL; in bile-fistula rats, monensin caused a decrease of bile flow, altered protein profile and bile acids secretion. Because the effects of monensin seem to be complicated, in this note it has been studied the action of monensin on the morphology of liver cells to have a picture not only of the parenchymal cells, but also of the sinusoidal cells, which cooperation with the general liver functions become increasingly evident. The results obtained after staining of liver biopsies with Sudan Black, Sudan III and Sudan IV showed that monensin induces a diffuse cholestasis and islands of hepatocytes in steatosis, in agreement with the data in literature. Cells lining the sinusoid with phagocytic activity and that might be identified with fat-storing cells, showed an increase of their lipid droplets that might be attributed to vitamin A.
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PMID:[Morphologic changes in the liver of rats treated with monensin]. 228 46

Selective staining with Sudan IV and methylene blue for light microscopy and scanning electron microscopy (SEM) were investigated to determine their potential for detecting and quantifying microencapsulated insecticides. Penncap-M (microencapsulated methyl parathion), Penncapthrin (microencapsulated permethrin), and Dyfonate (microencapsulated fonofos) were selectively stained with Sudan IV but not with methylene blue. Selective staining was not possible for Altosid SF-10 or SR-20 (microencapsulated methoprene) with either stain. Sudan IV enabled detection of some microencapsulated formulations in the digestive content of selected aquatic invertebrates and prepared contaminated pollen samples. Staining intensity with Sudan IV was greatest with acetone but capsular damage was high. A solvent ratio of 50:50 and 20:80 acetone/xylene minimized capsular collapse and maintained good staining intensity. The use of SEM for capsule identification and quantification depended upon the method of sample preparation: the slide smear method was superior to samples prepared by incision or microtomy. SEM was most suitable for investigation of formulations such as methoprene, for which selective staining was not possible. The chemical basis of staining with Sudan IV and potential application of both identification techniques are discussed.
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PMID:Visual detection of microencapsulated insecticides with selective staining and scanning electron microscopy. 248 Sep 67