Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0338671 (Steroids)
 9,479 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The in vitro uptake of 2,4,6,7-tritiated estradiol-17beta in uterine eosinophils of the rat was inhibited by the presence of nonradioactive estradiol-17beta, estrone, and estriol, but not by progesterone, testosterone, or corticosterone. This action is attributed to competition between tritiated estradiol and the various estrogenic compounds for the same binding site. Compounds without any estrogenic activity do not compete. The proposal is made that the eosinophil binding system and the 8S-5S binding system are involved in different mechanisms of estrogen action. The parallelism between the doses of estradiol and estriol needed to promote certain estrogenic early effects in the uterus, and the affinity of these steroids for the eosinophil uptake sites, suggests that uterine eosinophils might be responsible for some of these early effects, such as water imbibition, histamine releasing activity, and estrogen priming effect.
Steroids 1972 Apr
PMID:Radioautographic study of the effect of estradiol-17 , estrone, estriol, progesterone, testosterone and corticosterone on the in vitro uptake of 2,4,6,7- 3 H estradiol-17 by uterine eosinophils of the rat. 502 83

Treating immature female rats with estradiol sc, 1 mcg in saline or .1 mcg in oil for 2 days, induced a peroxidase in the uterus which converted carbon-14 labeled estradiol into water soluble products in vitro. Uterine extracts prepared by Klebanoff's method were incubated for 1 hour at 38 degrees C with labeled estradiol, hydrogen peroxide, dichlorophenol, and bovine serum albumin in sodium ponsphate buffer. After extracting the medium with ether, the radioactivity in both phases was determined and the steroids in the ether phase were identified by thin layer chromatography. .1 mcg estradiol injected in saline did not affect either yield of estrone or water soluble metabolites. .1 mcg estradiol given in oil or 1 mcg in saline increased both the yield of soluble metabolites over fourfold and the percent conversion of estradiol to estrone sevenfold (oil) and twofold (saline). Thus estradiol is metabolized in the uterus; this metabolism may be a local control for duration of estrogen action.
Steroids 1972 Jul
PMID:Estrogen-induced metabolism of estradiol-17 in the rat uterus: a possible mechanism for the termination of estrogen action. 504 3

A double antibody enzyme immunoassay of plasma cortisol was established using beta-galactosidase from E. coli as the tracer. Cortisol-21-hemisuccinate was conjugated with beta-galactosidase using a water-soluble carbodiimide. An antibody raised in the rabbit against cortisol-21-hemisuccinate-bovine serum albumin was used as the first antibody and anti-rabbit gamma-globulin goat serum was used as the second. The sensitivity of the present enzyme immunoassay was 25 pg/tube. The method satisfied general reliability criteria regarding specificity, accuracy and precision. Plasma cortisol levels estimated by the enzyme immunoassay was highly correlated (r = 0.99, P less than 0.005) with the levels measured by radioimmunoassay. This method of enzyme immunoassay was found to be of practical application to the routine assay of plasma cortisol.
Steroids 1981 Jul
PMID:Double antibody enzyme immunoassay of cortisol in bovine plasma. 627 Aug 51

A novel synthesis of sodium 17-oxo-16 alpha-hydroxy-1,3,5(10)-estratrien-3-yl sulfate (4), sodium 16 alpha, 16 beta-dihydroxy-1,3,5(10)-estratrien-3-yl sulfate (5) and sodium 16-oxo-17 beta-hydroxy-1,3,5(10)-estratrien-3-yl sulfate (6) is described. 16 alpha-Bromo-3-hydroxy-1,3,5(10)-estratrien-17-one (1) was efficiently synthesized in one step with 70-97% yield by bromination of 3-hydroxy-1,3,5(10)-estratrien-17-one with cupric bromide. 3,16 alpha-Dihydroxy-1,3,5(10)-estratrien-17-one (3) was quantitatively obtained by controlled stereospecific hydrolysis of the bromoketone 1 with sodium hydroxide in aqueous pyridine. The bromoketone 1 was converted to the 16 alpha-hydroxy-17-ketone 3-sulfate 4 by sulfation with chlorosulfonic acid in pyridine and a subsequent controlled hydrolysis in a high yield without formation of the other ketols. Treatment of the sulfate 4 with sodium borohydride have the triol sulfate 5. The sulfate 4 was also rearranged to the 17 beta-hydroxy-16-ketone 6 with sodium hydroxide in water in a quantitative yield.
Steroids 1981 Nov
PMID:A short efficient synthesis of 16-oxygenated estratriene 3-sulfates. 627 76

Rats were maintained under standardized conditions of food and water ad libitum and a lighting schedule of 12 h light/we h dark for seven days. Animals were sacrificed at 0500 and 1700 h and tissues were removed and washed. Isolated intact adrenal and pituitary cells were prepared by collagenase treatment. Using a sequential incubation procedure, the release of pituitary ACTH by hypothalamic acid extracts (CRF) was assayed by stimulation of corticosteroid secretion from isolated adrenal cells. Maximum stimulation of adrenal steroids was achieved with hypothalamic extracts and pituitary cells from rats sacrificed at 1700 h, and minimum values were obtained at 0500 h. Intermediate levels were obtained when hypothalamic extracts at one time point were incubated with pituitary cells at the other. The results substantiate the late afternoon peak level of hypothalamic and pituitary secretion occurring prior to the peak activity pattern of the rat.
Steroids 1982 Nov
PMID:Hypothalamic and pituitary periodicity demonstrated by isolated rat adrenal cells. 631 42

The effect of elevated plasma concentrations of estradiol-17 beta (E2 beta), estrone (E1) and progesterone (P), in concentrations similar to those observed at the end of pregnancy, on the gonadotropin releasing hormone (GnRH)-induced luteinizing hormone (LH) release in postpartum dairy cows was studied. Twenty-five dairy cows in late gestation were assigned to five groups of five each to receive daily steroid treatments as follows: 1 and 2) no exogenous steroids; 3) 20 mg E2 beta and 30 mg E1; 4) 150 mg P and 5) 20 mg E2 beta + 30 mg E1 + 150 mg P. Steroids were dissolved in alcohol (vehicle) and injected sc twice daily. Cows receiving no steroids were given vehicle. Administration of steroids or vehicle began immediately after parturition (d 0) and continued for 7 d to maintain concentrations of steroids in plasma similar to prepartum concentrations. Cows in groups 2 through 5 received an injection of 100 micrograms GnRH on d 2, 8, 16, 24 and 32 postpartum, while those in group 1 received water (vehicle for GnRH) on the same days. Plasma for hormonal determinations was collected on alternate days beginning 10 d before the expected day of parturition, daily through the period of steroid treatments (d 0 to 6, postpartum) and on alternate days thereafter until d 40 postpartum. In addition, plasma was collected immediately before GnRH or water administration and at .5 h intervals thereafter for 4 h. Trends in response to treatment over days postpartum were studied by partitioning sums of squares due to linear, quadratic and cubic polynomial responses.(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:Alteration of the GnRH-induced LH release by steroids in postpartum dairy cattle. 635 75

Nuclear binding of corticosteroids in fish tissues was investigated in two target organs of the trout Salmo gairdnerii irideus: the liver and the intestinal mucosa. Incubation of intact nuclei with [3H]-cortisol or [3H]-dexamethasone failed to demonstrate high-affinity binding of these steroids to proteins. Exchange assay of [3H]-cortisol in high-salt nuclear extracts indicated an association constant Ka = 1.9 X 10(4) M-1 for intestinal mucosa and 2.1 X 10(4) M-1 for liver. In sea water-adapted trout, the association constant remained the same as in fresh water. These results extend previous observations obtained on the cytosol which showed that no high-affinity receptors could be disclosed in fish tissues using these two corticosteroids.
Steroids 1984 Apr
PMID:Nuclear binding of cortisol in intestinal mucosa and liver of a teleost fish (Salmo gairdnerii irideus). 652 51

Four 17 beta-esters of norethisterone (17 alpha-ethynyl-17 beta-hydroxyestr-4-en-3-one), formulated both as oily solutions and aqueous suspensions, were administered intramuscularly to rabbits and free plasma levels measured for periods up to 9 weeks. For all formulations of the compounds, the disappearance of norethisterone following peak plasma levels obeyed first-order kinetics. Since different slope values were obtained for different formulations of the same compound, the values reflected the release rates of the esters from the formulations. Fusion data, partition coefficients and solubilities of the compounds in 2,2,4-trimethylpentane and water were obtained and these properties were related to the biological activity of the formulations. For oily solutions, the differences in plasma levels were ascribed to the different partition coefficients of the esters between the oil and tissue fluids. For suspensions, the different activity in relation to an oily solution of an ester was related to the strength of intermolecular forces in the crystal lattice and to the relative thermodynamic activity in the two formulations. The results demonstrate that microcrystalline suspensions do not always have a longer duration of activity than oily solutions of the same compound after intramuscular injection.
Steroids 1983 Mar
PMID:Long-acting contraceptive agents: the influence of physico-chemical properties of some esters of norethisterone upon the plasma levels of free norethisterone. 665 83

Estradiol-17 beta labeled with deuterium in the positions 2 or 4 can be prepared from 2-chloromercurio-1,3,5(10)-estratriene-3,17 beta-diol 3-methyl ether 17-acetate or 4-chloromercurio-1,3,5(10)-estratriene-3,17 beta-diol, respectively, in refluxing CH3COO(2)H/(2)H2O. The same reaction performed on 4-acetoxymercurio-1,3,5(10)-estratriene-3,17 beta-diol afforded 2,4-dideuterio-estradiol-17 beta in good yields.
Steroids 1983 Jun
PMID:Synthesis of C-2 and C-4 deuterium-labeled estradiol-17 beta. 666 20

Certain bile acid oxazoline derivatives (100 microM), but not corresponding unconjugated bile acids (100 microM), were found to inhibit the growth of Eubacterium sp. V.P.I. 12708. The growth inhibition was correlated with the polarity of the steroid portion of the bile acid oxazoline. Primary cultures of adult rat hepatocyte monolayer cultures converted [7 epsilon-14C]methylchenooxazoline3 into MeOH-H2O soluble derivatives. Certain intestinal bacteria were capable of metabolizing [17 epsilon-14C]methylchenooxazoline as well as the MeOH-soluble hepatocyte derivative(s). These results suggest that bile acid oxazoline derivatives may undergo hepatic, as well as bacterial metabolism during enterohepatic circulation.
Steroids 1983 Jul
PMID:Metabolism of bile acid oxazoline derivatives by hepatocyte monolayer cultures and intestinal anaerobic bacteria. 667 75


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>