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Query: UMLS:C0338671 (Steroids)
 9,479 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The concentrations of aldosterone in the plasma and adrenal glands, the concentrations of sodium and potassium in the plasma and the hematocrit were estimated from birth to day 6 after birth in premature mice removed by Caesarean section on day 19 of pregnancy in comparison with newborn mice delivered spontaneously vaginally on day 20 of pregnancy. In premature mice, the plasma aldosterone concentrations increased twice: at birth after reanimation, then at 6 h after birth. The first increase at birth resulted probably from ACTH stimulation. Several factors could be involved in the peak at 6 h after birth: ACTH stimulation, the decrease in the level of sodium in the plasma and the increase in the hematocrit due to kidney immaturity of premature mice. The results suggest that the renin-angiotensin-aldosterone system is able to respond to stimulations in the first 6 h after birth in premature mice. The rise in the level of plasma aldosterone which has been found at birth in newborns delivered spontaneously vaginally on day 20 of pregnancy (control animals) did not result from variations of plasma electrolytes, plasma volume and ACTH; this rise has been induced by labor of the parturition which caused the aldosterone release from adrenal glands.
Steroids 1982 Sep
PMID:Plasma and adrenal aldosterone levels in premature mice at birth and during neonatal development. 718 5

The effect of sodium molybdate (NaMo) on the ability to detect progesterone and estrogen receptors from human breast cancers was studied. When NaMo was added at 20 mM to cytosols from human breast cancers, progesterone receptor showed major increases by sucrose density gradient centrifugation with tritiated R5020 as ligand. Increases in both 8S and 4S receptors, or in 8S alone, also occurred (reported figuratively). When NaMo addition was analyzed by dextran-coated charcoal method, binding also increased, converting some apparently negative cytosols to positive and increasing the amount of receptor in other positive samples. Additional estrogen receptor could also be measured in some cytosols, and a quantitative temperature-dependent coversion of 8S to 4S binding molecules could be achieved. NaMo prevented loss of binding in 30-degree-incubated cytosols in the absence of added estradiol. NaMo increased amount of progesterone receptor, and to a lesser extent estrogen receptor, but how and why are not known.
Steroids 1980 Mar
PMID:Sodium molybdate increases the amount of progesterone and estrogen receptor detected in certain human breast cancer cytosols. 718 11

The effect of sodium intake on the aldosterone metabolic clearance rate (MCR) was examined in 5 normal subjects. Measurements were made under conditions where dietary sodium ranged from 10 to 1500 meq/day. There were no consistent changes in MCR over these extremes of sodium intake, and the plasma level of aldosterone correlated only with the aldosterone urinary excretion rate. In an additional group of 6 normal subjects, a single dose of 100 meq sodium administered orally had no effect on the aldosterone MCR. The findings indicate that aldosterone metabolism is unaffected by sodium intake.
Steroids 1981 Jan
PMID:The effect of sodium on aldosterone metabolic clearance. 722 40

The synthesis of 11 alpha-hydroxyestrone, 11 alpha-hydroxy-9 beta-estrone, and 11 beta-hydroxy-9 beta-estrone are presented. The reduction of 11-keto-9 beta-estrone 17-ethyleneketal by sodium in ethanol or sodium borohydride resulted in 11-hydroxy-9 beta-estrones. The 11-hydroxyl group configurations were opposite to expectations: sodium in boiling ethanol afforded the axial 11 beta-hydroxy-9 beta-estrone, while sodium borohydride in boiling tetrahydrofuran gave the equatorial 11 alpha-hydroxy-9 beta-estrone. In immature rat uterotropic bioassays using subcutaneous injections, 11 alpha-hydroxyestrone was 2 times as active as 11 alpha-hydroxy-9 beta-estrone, and 11 beta-hydroxyestrone was 10 times as active as 11 beta-hydroxy-9 beta-estrone.
Steroids 1981 Mar
PMID:Structure-activity relationships of four 11-hydroxyestrones isomeric at the C-9 and C-11 positions. 723 53

A novel synthesis of 16 alpha-hydroxy-4-androstene-3,17-dione (3), 16 alpha-hydroxy-4-androstene-3,6,17-trione (4), 17 beta-amino-5-androsten-3 beta-o1 (10) and 17 beta-amino-4-androsten-3-one (14) is described. 16 alpha-Bromoacetoxy-4-androstene-3,17-dione (5), 16 alpha-bromoacetoxy-4-androstene-3,6,17-trione (6) and 17 beta-bromoacetylamino-4-androsten-3-one (15) were synthesized as potentially selective irreversible inhibitors of androgen aromatases. 16 alpha-Bromo-4-androstene-3,17-dione (1) and 16 alpha-bromo-4-androstene-3,6,17-trione (2) were converted to compounds 3 and 4 in 80-90% yield by controlled stereospecific hydrolysis using sodium hydroxide in aqueous pyridine. Reductive amination of 3 beta-hydroxy-5-androsten-17-one and 3-methoxy-3,5-androstadien-17-one (11) using ammonium acetate and sodium cyanohydridoborate (NaBH3CN) and a subsequent treatment with acid gave the amines 10 and 14 respectively, as a salt. The corresponding 17-imino compounds 9 and 13 were also isolated from the reaction mixtures when methanol was used as a solvent for the reaction. The 16 alpha-hydroxyl compounds 3 and 4 and the 17 beta-amino compound 14 were converted to the corresponding bromoacetyl derivatives, 5, 6, and 15, with bromoacetic acid and N,N'-dicyclohexylcarbodiimide.
Steroids 1981 Aug
PMID:Synthesis of 16 alpha-bromoacetoxy androgens and 17 beta-bromoacetylamino-4-androsten-3-one: potential affinity labels of human placental aromatase. 730 27

The reduction of 3-ethylenedioxy-7-oximino-5-androsten-17 beta-yl acetate and of its 17 beta-tetrahydropyranyl ether analog with sodium in ethanol, followed by thin-layer chromatography, allowed the isolation of the corresponding 17 beta-hydroxy- and 17 beta-tetrahydropyranyloxy-5-en-7 beta- and 7 alpha-amines which were also characterized as 7-acetamides. The acylation of the two epimeric 17 beta-hydroxy-5-en-7-amines with succinic anhydride followed by selective saponification of the 17 beta-hemisuccinate group and diazomethane esterification, gave the corresponding 17 beta-hydroxy-5-en-7 beta- and 7 alpha-hemisuccinamido methyl esters characterized also as 17 beta-acetates. On the other hand, the acylation of the two 17 beta-tetrahydropyranyloxy-5-en-7-amines with the acid chloride of terephthalic acid monomethyl ester led to the more rigid 7 beta- and 7 alpha-terephthalamido methyl ester side-chains. The acidolysis of the 3-ethyleneketal protecting group of the preceding 5-en-7-N-acyl derivatives regenerated the 4-en-3-oxo function while the 17 beta-tetrahydropyranyl ether group was cleaved simultaneously into the 17 beta-alcohol. The four desired 7 beta- and 7 alpha-hemisuccinamido- and terephthalamido carboxylic side-chain derivatives of 17 beta-hydroxy-4-androsten-3-one (testosterone) were finally obtained by saponification of the corresponding methyl esters.
Steroids 1981 Dec
PMID:Synthesis and stereochemistry of 7 beta- and 7 alpha-amino-, acetamido-, hemisuccinamido- and terephthalamido derivatives of testosterone. 733 62

Seventeen cholesteryl alkyl ethers were synthesized through alcoholysis of cholesterol p-toluenesulfonate. This method was found superior to the etherification of sodium or potassium cholesterylate with alkyl halides or methanesulfonates, especially for the preparation of long-chain unsaturated aklyl ethers of [7(m)-3H]cholesterol of high specific activity.
Steroids 1980 Jan
PMID:The synthesis of cholesteryl alkyl ethers. 737 10

A method for the production of the haptens 18-hydroxy-11-deoxycorticosterone-3-(O-carboxymethyl)-oxime (18-OH-DOC-3-CMO) and 18-hydroxycorticosterone-3-(O-carboxymethyl)-oxime (18-OH-B-3-CMO) is described. The formation of the oximes was studied in kinetic experiments. They were prepared at pH 1.6 in methanol/HCl using a short reaction time. Antisera were raised in rabbits using serum albumin conjugates. The highly specific antisera were used at a final dilution of 1:79 000 (18-OH-DOC) and 1:43 000 (18-OH-B); the affinity constants were 1.2 x 10(10) l/mol and 8.1 x 10(9) l/mol, respectively. The radioimmunoassay procedure for 18-OH-B in serum involves purification by paper chromatography. The intra- and interassay precision was 7.3% and 12.3%, respectively. The mean serum 18-OH-B level (+/- S.D.) for normal male and female ambulatory subjects (n = 40) on a normal sodium diet was 0.802 +/- 0.262 nmol/l. After 60 minutes of recumbency, the serum 18-OH-B level was 0.313 +/- 0.061 nmol/l (n = 6) for men.
Steroids 1980 Apr
PMID:Development and characterization of antisera to 18-hydroxycorticosterone and 18-hydroxy-11-deoxycorticosterone and radioimmunoassay for serum 18-hydroxycorticosterone. 737 29

Portions of pregnancy and midcycle urines were submitted to hot acid hydrolysis, extracted with benzene/ethyl acetate and the extracts washed with ascorbic acid buffer. From the remaining organic phase the catecholestrogens were removed with borate buffer and further purified on Sephadex LH-20 columns. After derivatisation 4-hydroxyestrone was separated from the isomeric 2-hydroxyestrone peak were identical with that of authentic 4-hydroxyestrone. After treatment of the extracts with sodium borohydride 4-hydroxyestradiol-17 beta was identified by GC-MS. By the addition of trace amounts of tritiated 4-hydroxyestrone a recovery of 40% was calculated. On the basis of this recovery and the peak heights of the gas chromatograms an excretion of 4 microgram (midcycle) and 40 microgram (pregnancy) of 4-hydroxyestrone/24 h was estimated.
Steroids 1980 Jul
PMID:4-hydroxyestrone, isolation and identification in human urine. 741 57

19-Hydroxydeoxycorticosterone (19-OH-DOC) was isolated from the incubation medium of enucleated rat adrenal glands during the early sodium retaining phase. Identification included comparison of chromatographic mobility of parent and derivatized compound with standard prepared by the 21-hydroxylation of 19-hydroxyprogesterone and mass spectrometry. The possible role of 19-OH-DOC as a precursor is discussed.
Steroids 1980 Nov
PMID:Identification of 19-hydroxydeoxycorticosterone in regenerating rat adrenal incubations. 745 3


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