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Query: UMLS:C0338671 (Steroids)
 9,479 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cell density, nutritional state, and serum factors modify the growth response of LNCaP human prostatic cancer cells to dihydrotestosterone. Evaluation of growth response to dihydrotestosterone requires logarithmic transformation of cell count or thymidine incorporation data. Under conditions of dose response, growth increases with cell density but no significant interaction of dihydrotestosterone with cell density was found under optimal culture conditions. The frequency of media change was a significant factor in modulating dose response. When cells from cultures maintained at different feeding periods were plated at different cell densities of (trypan blue) viable cells, significant effects of plating density on dihydrotestosterone response were found. Dihydrotestosterone protects cells under the adverse effects of media deprivation. Under the extreme adverse effects of serum deprivation, cells respond to dihydrotestosterone even under conditions of increasing cell loss. The effects of dihydrotestosterone on final cell density were significant. In the absence of serum, the elongated cells of LNCaP assume a round shape, but many remain adherent to the culture dish and can be restored to normal morphology by serum. A number of growth factors fail to restore normal morphology that was completely restored by a combination of fibronectin and dihydrotestosterone. We have not developed a practicable serum-free system for LNCaP.
Steroids 1992 Jun
PMID:The effect of dihydrotestosterone and culture conditions on proliferation of the human prostatic cancer cell line LNCaP. 144 Jun 97

In the present work, we studied the possible effect of steroid hormones, estradiol, progesterone, and 5 alpha-dihydrotestosterone, on different phenotypic and functional characteristics of peritoneal adherent mononuclear cells. We used female and male mice of Balb/c strain, normal, gonadectomized, and gonadectomized with hormonal replacement. We found that gonadectomy in both sexes produced a significant decrease in the functionality of membrane receptors for the complement and in phagocytic activity of Candida albicans-anti-C albicans system. In addition, the percentages of cells that reduced nitroblue tetrazolium were diminished in castrated animals. Ovariectomized females injected with estradiol presented normal levels of phagocytic and metabolic capacities, but the expression of membrane receptors for complement remained decreased. In contrast, progesterone treatment of ovariectomized animals had the opposite effect. Simultaneous treatment with estradiol plus progesterone gave results similar to those observed with estradiol only. Dihydrotestosterone per se did not affect any of the parameters measured in the conditions used here. These results suggest that female steroids affect macrophage functionality, probably by regulating surface receptors that are involved in phagocytic activity.
Steroids 1991 Sep
PMID:Effects of sexual steroid hormones on the functionality of murine peritoneal macrophages. 180 61

Dihydrotestosterone-succinyl-agarose is the most common form of androgen affinity column. To investigate the effect of variation in the number of methylene groups between the ester and amide functions, a homologous series, varying the number of methylenes between the functional groups, has been synthesized and evaluated. In addition, since structure studies show 17 alpha-methyldihydrotestosterone binds with high affinity, a 17 alpha-carboxymethyl ligand (3) was studied. Relative binding affinities of the dicarboxylates (assayed as the n-butyl amides) range from 0.003 to 0.044 (dihydrotestosterone = 1.00), while there was no detectable binding for 3. Only the suberate binds better than the much-used succinate, and it would be a likely candidate for an affinity ligand.
Steroids 1990 Jun
PMID:Synthesis and androgen receptor binding of dihydrotestosterone hemisuccinate homologs. 238 49

Perfusion of canine prostatic tissue with [1, 2-3H] 5 alpha-androstane-3 alpha, 17 beta-diol and [4-14C] dihydrotestosterone and the measurement of the isotopic concentrations at the steady state were used to calculate the metabolic dynamics of these steroids by prostatic tissue obtained from normal, castrated or androgen-treated dogs. From the results it was concluded that: Entry of both steroids into the tissue was similar, and no saturation was observed with increasing concentrations of these androgens in the perfusion medium. In contrast, the entry of both steroids was reduced when the perfusion buffer was replaced with diluted plasma. Dihydrotestosterone in prostatic tissue was present in a diffusible form, whereas 3 alpha,17 beta-androstanediol was not. The conversion of 3 alpha, 17 beta-androstanediol to dihydrotestosterone was always higher than the conversion of dihydrotestosterone to 3 alpha, 17 beta-androstanediol; thus, the oxidative pathway was favored. The entry and uptake of both androgens was greatly reduced in those prostates excised from castrated dogs. The uptake of dihydrotestosterone by normal prostatic tissue was 3- to 6-fold higher than the uptake of 3 alpha, 17 beta-androstanediol. However, higher intratissular concentrations of dihydrotestosterone were obtained by increasing the concentration of 3 alpha, 17 beta-androstanediol perfused over that observed by increasing the concentration of dihydrotestosterone perfused. In the hyperplastic tissue, the entry, uptake and metabolism of the two androgens were similar to those observed in the normal gland, but their intratissular concentrations were found to be higher.
Steroids
PMID:Dihydrotestosterone and 3 alpha-androstanediol dynamics in the normal, involuted, and hyperplastic canine prostate. 244 22

Gas chromatography/mass spectrometry with selected ion monitoring has been employed to examine extracts from the ovary, eggs, and haemolymph of the marine prawn, Nephrops norvegicus, to demonstrate the presence of steroids. Both free and conjugated steroids were isolated by solvent partitioning and chromatography (lipophilic Sephadex, reversed-phase Sep Pak, and normal phase medium-pressure liquid chromatography) and steroidal conjugates were cleaved enzymatically. Steroids were determined as their methyloxime derivatives, trimethylsilyl (TMS) ethers or methyloxime-TMS ethers. All assignments were based on the detection of characteristic ions and cochromatography with the authentic steroid derivatives. 5 alpha-Dihydrotestosterone, testosterone, pregnenolone, and 20 alpha-hydroxypregn-4-en-3-one were detected in unconjugated form in the ovary. The eggs and haemolymph were found to contain unconjugated 17 beta-estradiol. Conjugated 5 alpha-dihydrotestosterone was detected in both the ovary and haemolymph, but no conjugated steroids were found in the eggs.
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PMID:Detection of unconjugated and conjugated steroids in the ovary, eggs, and haemolymph of the decapod crustacean Nephrops norvegicus. 271 24

3 beta,20 alpha-Hydroxysteroid oxidoreductase has been isolated from ovine fetal blood by a 2,370-fold purification scheme of ammonium sulfate fractionation, calcium phosphate gel adsorption, affinity chromatography, and fast performance liquid chromatography. A new high performance liquid chromatography-based assay for measuring 20 alpha-reductase activity is described. The enzyme is a monomer with a molecular weight of 35,000 and uses NADPH as a cofactor for reductase activity. It reduces progesterone to 4-pregnen-20 alpha-ol-3-one or 5 alpha-dihydrotestosterone to 5 alpha-androstan-3 beta,17 beta-diol with kinetic characteristics of Km = 30.8 microM and Vmax = 0.7 nmol min-1 (nmol of enzyme)-1 or Km = 74 microM and Vmax = 1.3 nmol min-1 (nmol of enzyme)-1, respectively. 5 alpha-Dihydrotestosterone competitively inhibits 20 alpha-reductase activity with a Ki value of 102 microM.
Steroids 1987 Jun
PMID:Isolation of 3 beta,20 alpha-hydroxysteroid oxidoreductase from sheep fetal blood. 348 95

The effects of some androgenic-myotrophic steroids on pubertal ovulation in rats were studied. Treatment began at age 32 days. Steroids were given subcutaneously for 14 consecutive days. Autopsy was on the fifteenth day. The control animals showed an ovulation rate of 98% (137/140 rats). All of the steroids tested suppressed ovulation but differed in their potencies. Testosterone (7 rats ovulating/10 in group), dihydrotestosterone (5/10), nortestosterone (4/10), and norethandrolone (6/10) showed inhibitory effects at a dose of 20 micrograms/day. Nortestosterone seemed most effective, reducing ovulation to 10% at a daily dose of 40 micrograms. Androstenedione, methandrostenolone, oxymetholone, oxandrolone, ethylestrenol, and stanozolol required doses of 100 micrograms or more to depress ovulation. With most of the steroids the ovarian weight depression correlated exactly with ovulation suppression in regard to dose. Half of the compounds decreased uterine weight at lower doses but increased it at higher doses within the dosage range (1-4000 micrograms/day). Dihydrotestosterone, oxymetholone, and ethylestrenol caused uterine weight depression, norethandrolone caused uterine weight stimulation, and oxandrolone had no significant effect on this variable. All of the compounds except testosterone, methandrostenolone, and oxymetholone failed to increase body weight. The ovulation suppression, reduced ovarian weight, and reduced uterine weight observed probably are associated with antigonadotropic activity. For purposes of human contraception it is hoped a separation can be achieved by molecular modification between androgenicity and antiovulatory activity.
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PMID:Anti-ovulatory effects of some androgenic-myotrophic steroids in the pubertal rat. 465 Jun 62

The effect of various hormones on the levels of poly(A) polymerase in the ventral prostate of castrated rats was investigated. It was observed that this enzyme is specifically induced by androgens; progesterone and estradiol-17 beta did not cause stimulation of poly(A) polymerase activity. Dihydrotestosterone-induced poly(A) polymerase was inhibited by cordycepin, actinomycin-D and cycloheximide, which indicates that the genetic transcription leading to the enzyme poly(A) polymerase is regulated by androgens in the ventral prostate.
Steroids 1984 Apr
PMID:Androgen regulation of poly(A) polymerase in the ventral prostate of rat. 609 56

5 alpha-Dihydrotestosterone, 17-hydroxyprogesterone caproate, 2-methoxyestrone and a number of nonsteroidal antiestrogens (clomiphene citrate, nafoxidine hydrochloride, tamoxifen, MER-25) were tested for their ability to block estradiol-mediated repression of the androgen-dependent 3 beta-hydroxysteroid dehydrogenase activity of male rat liver. With the exception of 5 alpha-dihydrotestosterone, which induced activity in females, none of these substances affected 3 beta-hydroxysteroid dehydrogenase activity when administered alone to otherwise untreated male and female rats. Tamoxifen (100 or 500 micrograms/day) was the only substance which prevented a decrease in enzyme activity when given simultaneously with estradiol (5 micrograms/day). The estradiol-mediated decrease in activity was not antagonized by a 100-fold higher dose of androgen (5 alpha-dihydrotestosterone, 0.5 mg/day), demonstrating the potent antiandrogenic effect of estradiol on this hepatic androgen-dependent enzyme activity.
Steroids 1980 Nov
PMID:Antagonism of the estradiol-mediated repression of microsomal 3 beta-hydroxysteroid dehydrogenase activity in rat liver by antiestrogenic substances. 693 24

Concentrations of the neuropeptide arginine vasotocin (AVT) are sexually dimorphic in some regions of the bullfrog brain. Since these differences in AVT content may be due to sexual differences in plasma steroid concentrations, we performed a gonadectomy-steroid replacement experiment (30-day treatment) to assess the effects of specific steroids on AVT concentrations (determined by RIA) in adult male and female bullfrogs (Rana catesbeiana). These treatments had significant effects in six brain areas. Gonad removal decreased AVT concentrations in the amygdala pars lateralis, septal nucleus and habenula of both sexes. Gonadectomy decreased AVT content in optic tectum, torus semicircularis and pretrigeminal nucleus of males only. Dihydrotestosterone treatment resulted in AVT concentrations at or above sham levels in all six areas, whether or not gonadectomy decreased content. Estradiol fully restored AVT concentrations only in the septal nucleus and habenula (of both sexes) and in the amygdala of females. Estradiol partially restored AVT levels in the amygdala of males. These results indicate that the gonads maintain AVT concentrations in several brain areas and that steroids can modulate levels of AVT in bullfrog brain. Effects of each steroid vary depending upon the region and sex of the frog. AVT cells are present in three of these regions--the amygdala pars lateralis, septal nucleus and pretrigeminal nucleus. Steroids may be directly affecting AVT synthesis in these cells and these populations may be the source of AVT fibers in the other steroid-sensitive areas.
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PMID:Gonadal steroid modulation of vasotocin concentrations in the bullfrog brain. 796 72


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