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Query: UMLS:C0278080 (physical dependence)
 1,658 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Rats made dependent on ethanol by a schedule-induced polydipsia procedure preferred 5% ethanol to an increasing concentration of dextrose solution to a greater extent than animals on a non-dependent, non-polydipsic procedure which allowed an equivalent opportunity to drink ethanol, confirming a previous study. Two corresponding groups of animals drinking isotonic (0.9%) NaCl rather than 5% ethanol behaved similarly to the latter group, changing to a dextrose preference at a lower dextrose concentration than the ethanol polydipsic group. Therefore, neither the intermittent food regimen (polydipsia-generating procedure) in itself, nor a history of isotonic saline polydipsia biased fluid preference against dextrose solution choices. The enhanced preference for ethanol over dextrose solutions shown by the ethanol polydipsic group can be attributed to physical dependence rather than regiment produced artifacts.
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PMID:Ethanol dependence as a determinant of fluid preference. 56 2

Two recently developed methods for estimating changes in presynaptic gamma-aminobutyric acid (GABA) homeostasis were used for the first time to evaluate the effects of acute and chronic ethanol treatments on GABA utilization. GABA accumulation in the left substantia nigra zona reticulata (SNR) following unilateral microinjection of gamma-vinyl GABA (GVG; 5 micrograms) was linear for at least 180 min while GABA concentrations in the uninjected right SNR did not change over this period. Net GABA accumulation (left minus right SNR) also increased linearly over this interval. Intraperitoneal (i.p.) administration of ethanol (0.3, 1 or 3 g/kg) 15 min after GVG microinjection did not significantly change either the rate of GABA accumulation in left SNR, the net GABA accumulated or the concentration of GABA in the uninjected right SNR relative to saline injected controls over the 45-min test interval. Likewise, GABA accumulation in the left SNR or steady-state GABA concentrations in the right SNR of chronically intoxicated rats or physically dependent animals withdrawn from ethanol for 12 h did not change significantly from that dextrose-fed controls. In a separate study, the effects of acute and chronic ethanol treatments on the concentration of GABA in synaptosomes isolated from the frontal cortex, hippocampus, tectum, striatum, cerebellum or brainstem were determined. Thirty min after acute treatment with ethanol (0.5, 1, 2 or 4 g/kg, i.p.) the concentration of GABA in synaptosomes from any of these brain regions was not significantly altered. Furthermore, chronic ethanol treatment sufficient to induce physical dependence and a severe ethanol withdrawal syndrome also did not significantly modify synaptosomal GABA concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Effect of ethanol on gamma-vinyl GABA-induced GABA accumulation in the substantia nigra and on synaptosomal GABA content in six rat brain regions. 339 59

The effect of acute and long term administration of diazepam upon local cerebral glucose utilization (LCGU) in discrete regions of the central nervous system of the rat was studied by means of a quantitative autoradiographic [14C]2-deoxy-D-glucose technique. A single injection of diazepam (1.0 mg/kg and 2.5 mg/kg i.v.) reduced LCGU up to 30% in particular brain areas, such as the lateral and ventral thalamic nuclei, the medial geniculate body and the mamillary body, whereas the activity in many other structures was not significantly altered. That the effects may be mediated via specific benzodiazepine receptors was indicated by the ability of the selective benzodiazepine antagonist Ro 15-1788 (imidazobenzodiazepine) to attenuate these actions. Rats treated chronically with diazepam (20 mg/kg day for a period of 14 days) still displayed slight reductions of LCGU in certain areas affected acutely such as the lateral thalamic nucleus. In contrast to its effects in rats chronically treated with vehicle, an acute diazepam injection failed to significantly modify LCGU in rats chronically treated with diazepam. Furthermore, in chronic diazepam-treated animals Ro 15-1788 produced an increase of LCGU to values above control levels in those brain regions in which a decrement was seen upon acute diazepam administration to naive rats. These findings indicate an adaptation to and a possible development of physical dependence upon chronic drug treatment.
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PMID:Specific changes in local cerebral glucose utilization in the rat brain induced by acute and chronic diazepam. 393 75

Although the hamster generally prefers alcohol at a level similar to that of the rat or mouse selectively bred to consume alcohol, the drinking hamster demonstrates neither physical dependence on alcohol nor elevated blood levels of alcohol, which are two typical criteria characterizing an animal model of alcoholism. The present investigation was designed to determine whether a third criterion of an animal model (i.e., consumption of high levels of alcohol in the presence of a palatable fluid, fulfilled by the P rat) would be met by the female Syrian golden hamster (Mesocricetus auratus). A standard 3-bottle preference test was undertaken in 6 female hamsters over an 11 day period, in which water was presented in one tube and, in a second tube, a v/v solution of alcohol which was increased in concentration from 3% to 50% on each day as follows: 3%, 5%, 7%, 9%, 12%, 15%, 20%, 25%, 30%, 40%, and 50%. Then each hamster was offered its individually determined, maximally preferred concentration of alcohol for 4-8 days, which was 20%, 25%, or 30% alcohol. The mean absolute intake of alcohol during this period was 17.9 +/- 1.1 g/kg per day, whereas the mean proportion of alcohol to total fluid was 0.68 +/- 0.05. Then over a 4-day interval, a solution of tomato juice, peach juice, mango juice, dextrose and a chocolate beverage (Ensure Plus), all made isocaloric to the alcohol solutions with dextrose, was placed in the third tube simultaneously with water and the individually preferred concentration of alcohol.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Tomato juice, chocolate drink, and other fluids suppress volitional drinking of alcohol in the female Syrian golden hamster. 765 37

Planarians (Dugesia dorotocephala) that were exposed for 1 h to cocaine (80 microM) or to the kappa-selective opioid receptor agonist U-50,488H (1 microM) displayed an abstinence-induced withdrawal syndrome, indicative of the development of physical dependence, when they were tested in cocaine- (or U-50,488H-) free water, but not when they were tested in cocaine- (or U-50,488H-) containing water. The withdrawal was manifested as a significant (P<0.05) decrease in the rate of planarian spontaneous locomotor activity over a 5-min observation period, using a recently designed metric. Co-exposure of the planarians to D-glucose (1 microM) or to 2-deoxy-D-glucose (2-DG, 1 microM), but not to L-glucose (1 microM), significantly attenuated (P<0.05) the development of physical dependence, shown by an attenuated withdrawal syndrome, from cocaine and U-50,488H. These results suggest that either D-glucose and 2-deoxy-D-glucose compete with a common cocaine and kappa-opioid transport mechanism or that the development of physical dependence (or the inhibition of abstinence-induced withdrawal) in planarians requires energy supplied from glucose metabolism.
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PMID:Cocaine and kappa-opioid withdrawal in Planaria blocked by D-, but not L-, glucose. 1527 76

The domestic pig (Sus scrofa domesticus) has been proposed as an animal model for human alcoholism because pigs have been observed to consume alcohol voluntarily to a state of intoxication and to exhibit tolerance and physical dependence. However, it has not been established whether pigs can develop psychological dependence on alcohol. We hypothesised that feed-restricted, stall-housed pregnant sows fed alcohol non-voluntarily for 5 weeks would develop a preference for alcohol and retain this preference after removal of alcohol from the diet. We fed crossbred commercial sows (n=10) 280 ml of 95% ethanol mixed with 0.91 kg of feed and 720 ml of water twice daily for 5 weeks during the first trimester of pregnancy. Control sows (n=7) received dextrose in their feed as a caloric control, and water was added to give the feed a consistency similar to that of the alcohol-treated feed. Immediately before and after 5 weeks of alcohol or dextrose treatment and 3 weeks later, after termination of alcohol or dextrose treatment, we evaluated sow diet preference by comparing the amount of alcohol-supplemented, dextrose-supplemented and plain feed consumed during a 5-min choice test. Contrary to our hypothesis, there was no treatment effect on sow diet preference. Both alcohol-treated and control sows ate less of the alcohol diet than the other two diets in all choice tests. They did not discriminate between the plain and dextrose diets. We conclude that 5 weeks of non-voluntary consumption of alcohol in feed did not produce a preference for alcohol in pregnant sows, either during treatment or after withdrawal, thus providing no evidence for the development of psychological dependence on alcohol under these conditions.
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PMID:Consumption of alcohol by sows in a choice test. 1663 Dec 15