Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0277787 (stigma)
 13,352 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Self-incompatibility in Brassica oleracea is controlled by a single genetic locus (the S locus) with nearly 50 different alleles. In this paper, we report the characterization of the S2 allele, a pollen recessive self-incompatibility allele that exhibits weak DNA homology to the other previously sequenced S locus glycoprotein genes (SLG-6, -13, -14, -22 from alleles S6, S13, S14 and S22, respectively). Stigma cDNA clones with sequence homology to SLG-13 were isolated from two different S2 homozygous strains belonging to two different B. oleracea cultivars, var. alboglabra (Chinese kale) and var. italica (broccoli). The two S2 cDNA sequences are 90% homologous to each other, but only 70% homologous to SLG-13. Using the Chinese kale S2 genetic background, we demonstrate that the isolated alboglabra cDNA sequence is a transcript from a gene, designated SLG-2A that resides at the S locus, and propose that it is a putative determinant of S2 allelic specificity. Among the estimated 10-15 genomic copies of SLG-related genes detected in the S2 genome, we cloned and characterized the SLG-2A gene and another closely related and genetically linked gene copy, SLG-2B. A complete open reading frame that is 94% homologous to SLG-2A is located within SLG-2B. The existence of this intact duplicated S gene raises the possibility that SLG-2B may also be involved in the functioning of self-incompatibility in Brassica oleracea.
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PMID:A new class of S sequences defined by a pollen recessive self-incompatibility allele of Brassica oleracea. 198 Mar 34

182 plants representing 7 cultivars of Cape broccoli were tested for their S-alleles using the standard Brassica S-allele collection. In all but 12 plants both S-alleles were identified. A total of 20 different S-alleles were found, 9 of which occurred in the autumn types but not in the spring, 5 of which occurred in the spring types and not the autumn, and 6 of which were common to both. Most of the S-alleles detected in Cape broccoli were also found in Brussels sprouts or kales, but of the three which were not, two were confined to Cape broccoli while the third occurred only in Cape broccoli and Savoy cabbage. By far the commonest S-allele in Cape broccoli was S2, with an overall frequency of 35%. Three S-alleles were found (S14, S25 & S45) which were completely recessive in the stigma in certain combinations. The general pattern of distribution of S-alleles in Cape broccoli, with highly unequal frequencies, was similar to that in Brussels sprouts. The results indicate a high level of outcrossing in Cape broccoli which has implications for the breeding of this crop.
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PMID:Distribution of S-alleles and breeding structure of Cape broccoli (Brassica oleracea var.'italica'). 2430 Dec 11