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Query: UMLS:C0276640 (TEM)
 20,729 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Some peculiar ultrastructural aspects of hairy cells obtained from the examination with SEM and TEM are presented. Images of erythrocyte rosette-formation around hairy cells in spleen as well as some additional data on the biogenesis of ribosome-lamellae complexes are reported. Some considerations on the origin of hairy cells are added.
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PMID:Particular ultrastructural aspects in some splenectomized cases of hairy cell leukemia. 624 Jun 4

The surface structure of the hydrocarbon-utilizing yeast Candida tropicalis was investigated by scanning and transmission electron microscopy (SEM and TEM respectively). The sample preparation technique was based on a rapid cryofixation without any addition of cryoprotectants. In subsequently freeze-dried samples the surface structure was analysed by scanning electron microscopy. Thin sections were prepared from freeze substituted samples. Both techniques revealed hair-like structures at the surface of hydrocarbon-grown cells. The hairy surface structure of the cells was less expressed in glucose-grown cells and it was absent completely after proteolytic digestion of the cells. When cells were incubated with hexadecane prior to cryofixation a contrast-rich region occurred in the hair fringe of thin sections as revealed by TEM. Since these structures were characteristic for hexadecane-grown cells and could not be detected in glucose-grown or protease-treated cells it was concluded that they originate from hexadecane adhering to the cell surface and are functionally related to hexadecane transport. The structure of the surface and its relation to hydrocarbon transport are discussed in view of earlier results on the chemical composition of the surface layer of the cell wall.
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PMID:Structure of the cell surface of the yeast Candida tropicalis and its relation to hydrocarbon transport. 647 32

Two cases of hairy cell leukemia have been studied by immuno-TEM and immuno-SEM after immunogold labelling of the cell surface antigen recognized by the B-ly7 monoclonal antibody. Most hairy cells appeared significantly labeled, although the density of the expression of the antigen, as demonstrated by immunogold labelling, seems variable from cell to cell. Moreover, some cells with the morphology of hairy cells and which could not be identified as monocytes were not labeled. Labelling for the antigen identified by the B-ly7 mAb does not seem to correlate with the presence of ribosome lamellae complexes which were present only in one of the two cases studied. Rare lymphocytes of unidentified lineage were labeled. Monocytes were significantly absent from the samples of peripheral blood of the two patients studied. In one normal control sample, monocytes were observed unlabelled. The results are discussed in reference to the pathogenesis of hairy cell leukemia, its surprisingly low mitotic rate, and its distinct response to chemotherapy.
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PMID:Immunogold labelling of leukemic hairy cells with the B-ly7 monoclonal antibody: an SEM and TEM study. 791 22

Human ovarian carcinoma cells (MDAH 2774) were treated with sodium ascorbate (VC), menadione (VK3), or with a VC:VK3 combination for 1 h and then studied using light microscopy (LM) and scanning (SEM) and transmission electron (TEM) microscopy. Plasma membrane damage (blisters and blebs, hairy aspect) results from vitamin C (VC) treatment, while cytoskeletal damage and self-morsellation are caused by vitamin K3 (VK3) treatment. VC:VK3-treated cells exhibit exacerbated injuries characteristic of both VC and VK3 treatment as well as a significant decrease in cell diameters from 20-35 microm for control cells to 7-12 microm for VC:VK3 treatment. Moreover, after a 1-h exposure to the vitamin combination, autoschizis (43%), apoptosis (3%), and oncosis (1.9%) are observed at the percentages indicated. All cellular changes associated with autoschizis observed with SEM were confirmed by LM and TEM observations and are consistent with cell death by autoschizis: decrease in cell size, cytoplasmic self-excisions, degradation of the nucleus and nucleolus without formation of apoptotic bodies and, ultimately, karyorrhexis and karyolysis. These results also suggest that the vitamin combination may find clinical use in the treatment of ovarian cancer.
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PMID:Microscopic aspects of autoschizic cell death in human ovarian carcinoma (2774) cells following vitamin C, vitamin K3 or vitamin C:K3 treatment. 1290 65

We report for the first time on the design of an advanced hairy hybrid Janus-type catalyst, which is comprised of an inorganic silica core covered with two distinct polymeric shells (hydrophilic and hydrophobic) on its opposite sides, while the catalytic species (in our case silver or gold nanoparticles) are immobilized directly into the hydrophilic stimuli-responsive polymer shell. The primary 200 nm large Janus particles with poly(acrylic acid) serving as the hydrophilic and polystyrene as the hydrophobic polymer were synthesized through a Pickering emulsion and a combination of "grafting from"/"grafting to" approaches. The incorporation of silver and gold nanoparticles within the hydrophilic polymer shell was achieved by infiltrating the respective metal ions into the polymer matrix, and nanoparticles were formed upon the addition of a reducing agent (triethylamine). Plasmon absorptions typical for silver and gold nanostructures were observed on the functionalized Janus particles using UV-vis spectroscopy. The respective systems were investigated by TEM and cryo-TEM revealing that the incorporated nanoparticles are selectively localized on the poly(acrylic acid) side of the Janus particles. The efficiency of the catalyst as well as the accessibility of the incorporated nanoparticles was tested on the reduction of Methylene Blue, Eosin Y, and 4-nitrophenol as convenient benchmark systems. Ultimately, the hairy Janus particles with immobilized Ag or Au nanoparticles efficiently catalyzed the respective reactions by applying extremely low amounts of catalyst. Finally, we demonstrated several advantages of the use of JPs with immobilized metallic nanoparticles, which are (i) JPs stabilize the emulsions, (ii) the emulsion can be destabilized by utilizing responsive properties of the JPs, and (iii) JPs can easily be recovered after reaction and reused again.
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PMID:Hybrid Hairy Janus Particles Decorated with Metallic Nanoparticles for Catalytic Applications. 2635 69

Fruit features represent a trade-off between dispersal and protection against frugivore insects. To prevent insect attack, plants evolved chemical and physical barriers, mainly studied in leaves, while limited knowledge is available for fruits, especially concerning mechanical barriers. We used the Mediterranean fruit fly to shed light on the mechanical ecology of insect-fruit attachment in a pest species. We tested the following hypotheses: is there any sexual dimorphism in attachment devices and attachment ability? Can the attachment ability of females of Ceratitis capitata to fruits of various host plants vary according to fruit surfaces with different morphology (smooth, hairy, waxy) or physico-chemical properties? The tarsal attachment devices were studied using Cryo-SEM and TEM. The maximum friction forces of C. capitata females on fruit surfaces of typical host plants were evaluated using a load cell force transducer. The attachment ability of both sexes on artificial surfaces was evaluated using a centrifugal force tester. Our data revealed sexual dimorphism in the size of pulvilli, which are wider in females. A higher friction force is exerted by females in comparison with males, in agreement with the need to firmly adhere to the host plant fruit during oviposition. Among the tested fruits, the stronger friction force was recorded on hairy or rough surfaces while a force reduction was recorded on waxy fruits. To unravel the mechanical ecology of insect-plant interaction between plants and species of Tephritidae can be useful to develop non-chemical methods to control these important crop pests.
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PMID:Mechanical ecology of fruit-insect interaction in the adult Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae). 3207 16