Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0276640 (TEM)
 20,729 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The normal blood hepatocytic barrier (BHB), comprised by the endothelial lining cells and the perisinusoidal space, was investigated, and it was concluded that the BHB is a complex structure in which each individual part serves several functions. Immunohistochemical staining for factor VIII showed endothelial lining cells to be positive. By TEM and SEM the endothelial cells were seen to be fenestrated without a basal lamina. They possess numerous endocytotic vesicles. The perisinusoidal space contains the lipocytes and a matrix composed of fibrils, and a microfilamentous to granular material. The nature of these matrix components was poorly analyzable by conventional methods. Lipocytes were visualized by LM in: sections stained with oil red 0 after fixation in Baker's formol-calcium and following post-fixation in dichromate, and toluidine-blue-stained Epon-embedded sections. TEM revealed numerous cytoplasmic processes rich in microfilaments encircling the sinusoids.
 ...
PMID:The blood hepatocytic barrier: a light microscopical, transmission- and scanning electron microscopic study. 309 3

Structural features of isolated, fractionated rat bone marrow endothelium were compared to those of marrow sinus endothelium in situ. Marrow endothelium was purified, first by density gradient sedimentation on Percoll and then subjected to centrifugal elutriation. Using antifactor VIII antibody staining (indirect immunofluorescent method), preparations of greater than 50% purified endothelium were obtained. By SEM, these cells were about 10 microns in size and showed smooth surface and numerous invaginations. These features were also observed in the in situ endothelium obtained by perfusion-fixation and freeze-cracking. In addition, in situ endothelium displayed numerous hemopoietic cells in migration through the endothelium. By TEM, isolated endothelium showed numerous vesiculations, giving the cell sponge-like appearance. This corresponded to numerous intracellular vesicles in sinus endothelium in situ, reflecting high magnitude of fluid and molecular transport across the endothelium. Weibel-Palade bodies were not seen in either form of the endothelium, despite the positive reaction for factor VIII-related antigen. This finding suggested that the cell, while possessing factor VIII-related antigen, does not store this protein.
 ...
PMID:Structural features of isolated, fractionated bone marrow endothelium compared to sinus endothelium in situ. 379 99

Platelets were isolated from human blood by Percoll density gradient centrifugation in a low Ca2+/high Mg2+ buffer. The buffer reversibly inactivates the cells during separation. The purity of the isolated cells (> 99%) was determined by flow cytometry, their viability was confirmed by fluorescein diacetate hydrolysis, and their morphology was studied with TEM. Plasma proteins were adsorbed onto hydrophobic glass surface, and pure platelets were added and incubated for up to 30 min at 37 degree C. Platelet activation was determined by cell spreading, formation of microparticles and surface exposure of CD62P indicating the release of alpha-granules. Surface-immobilized IgG was shown to cause the release of microparticles and cell lysis, in accordance with data published by others. Surface-immobilized vWF was shown to induce CD62P exposure on the platelet cell surface. The specificity of this response was demonstrated by adsorbing plasma proteins from normal and factor VIII-deficient plasma.
 ...
PMID:Specific activation of platelets by surface-adsorbed plasma proteins. 972 96