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 20,729 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Proline-rich peptides are a chemically and structurally diverse family of cell-penetrating vectors characterised by the presence of pyrrolidine rings from prolines. Amphipathic Pro-rich peptides are particularly effective, demonstrating efficient cellular uptake and non-cytotoxicity. Derivatives with hydrophobic moieties, such as fatty acids or silaproline, have shown highly improved internalisation efficiency; an all D-amino acid version of the CPP SAP was shown to be completely protease resistant and was evaluated in a preliminary in vivo study. CD and TEM studies regarding the self-assembly properties of this family of peptides highlight the possible role of aggregated species in the internalisation process. Finally, these CPPs were shown to be internalised via caveolae or lipid-rafts mediated endocytosis, which circumvents the lysosomal route of degradation.
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PMID:Proline-rich, amphipathic cell-penetrating peptides. 1818 29

Cell-penetrating peptides (CPPs) are a potential tool for intracellular delivery of different kinds of cargoes. Because of their growing use in nanobiomedicine, both for diagnostics and for treatment, metal nanoparticles are an interesting cargo for CPPs. Here, gold nanoparticles (AuNps) and the amphipathic proline-rich peptide SAP have been used. Conjugation of the peptide onto the AuNps was achieved by addition of a cysteine to the SAP sequence for thiol chemisorption on gold, and the attachment was confirmed by visible spectroscopy, dynamic light scattering (DLS), zeta-potential (ZP), stability towards ionic strength (as high as 1 M NaCl), X-ray photoelectron spectroscopy (XPS) and high-resolution transmission electron microscopy (HR-TEM) coupled to electron energy loss spectroscopy (EELS). AuNp-C-SAP internalization in HeLa cells was observed by three different microscopy techniques-TEM, confocal laser scanning microscopy (CLSM) and transmission X-ray microscopy (TXM)-and all of them have confirmed the effective intracellular delivery of AuNps by SAP.
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PMID:Shuttling gold nanoparticles into tumoral cells with an amphipathic proline-rich peptide. 1932 42

Alkaptonuria (AKU) is an ultra-rare disease developed from the lack of homogentisic acid oxidase activity, causing homogentisic acid (HGA) accumulation that produces a HGA-melanin ochronotic pigment, of unknown composition. There is no therapy for AKU. Our aim was to verify if AKU implied a secondary amyloidosis. Congo Red, Thioflavin-T staining and TEM were performed to assess amyloid presence in AKU specimens (cartilage, synovia, periumbelical fat, salivary gland) and in HGA-treated human chondrocytes and cartilage. SAA and SAP deposition was examined using immunofluorescence and their levels were evaluated in the patients' plasma by ELISA. 2D electrophoresis was undertaken in AKU cells to evaluate the levels of proteins involved in amyloidogenesis. AKU osteoarticular tissues contained SAA-amyloid in 7/7 patients. Ochronotic pigment and amyloid co-localized in AKU osteoarticular tissues. SAA and SAP composition of the deposits assessed secondary type of amyloidosis. High levels of SAA and SAP were found in AKU patients' plasma. Systemic amyloidosis was assessed by Congo Red staining of patients' abdominal fat and salivary gland. AKU is the second pathology after Parkinson's disease where amyloid is associated with a form of melanin. Aberrant expression of proteins involved in amyloidogenesis has been found in AKU cells. Our findings on alkaptonuria as a novel type II AA amyloidosis open new important perspectives for its therapy, since methotrexate treatment proved to significantly reduce in vitro HGA-induced A-amyloid aggregates.
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PMID:Alkaptonuria is a novel human secondary amyloidogenic disease. 2285 Apr 26

Few studies have been conducted on the use of general nanotechnology-based principles for providing nutrients to crops. Water and fertilizer are the most important inputs in determining crop yield and profit. In this paper, super-absorbent polymers SAPWS (grafting wheat straw (WS) to poly(acrylic-co-acrylamide), which is WS-g-P(AA-co-AM)) and SAPHEC (HEC (hydroxyethyl cellulose)-g-P(AA-co-AM)) and their semi-interpenetrating polymer network (semi-IPN) nanocomposites SI-PSRF/SAPWS and SI-PSRF/SAPHEC (formed by chemical bonding of SAPWS or SAPHEC with PSRF (NPK-containing polymeric slow-release fertilizer)) were prepared. Due to the differences between activity and number of functional groups in WS and HEC, the water absorption properties and degradation performances of SAPWS and SAPHEC and their chemically bonded function nanocomposites SI-PSRF/SAPWS and SI-PSRF/SAPHEC are different. The maximum water absorption ratios of SAPWS, SAPHEC, SI-PSRF/SAPWS, and SI-PSRF/SAPHEC can reach 200.00 g/g, 240.00 g/g, 119.91g/g, and 127.43 g/g, respectively. Effects of the structures of these four materials on their degradation performances were studied via a tomato pot experiment. The changes in microstructures of these materials during the degradation processes were characterized by TEM, SEM, FTIR, XRD, XPS, and other techniques. Results show that the degradation rate of the chemically bonded functional nanocomposites with a semi-IPN structure SI-PSRF/SAP system is higher than that of SAP+PSRF (the simple physically mixed system of PSRF and SAP). Due to the differences among the activities and quantities of functional groups in WS and HEC, the water absorption properties of SI-PSRF/SAPWS and SI-PSRF/SAPHEC are different, and there are different degradation rates for these two functional nanocomposites.
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PMID:Novel Semi-IPN Nanocomposites with Functions of both Nutrient Slow-Release and Water Retention. 1. Microscopic Structure, Water Absorbency, and Degradation Performance. 3119 51