Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0276640 (
TEM
)
20,729
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Three human gingival fibroblast cell lines were used to determine whether they could be induced by a synthetic RNA and superinduced by metabolic inhibitors to produce interferon (
IFN-beta
). When established procedures were followed for human fetal or newborn skin fibroblast cell lines, the adult gingival fibroblasts produced comparable amounts of
IFN-beta
. It was shown that the superinducers alone would not cause an
IFN-beta
production response, and that the absence of serum in the production medium also inhibited the production of
IFN-beta
. The effect of
IFN-beta
on cell growth was carried out in T-flasks seeded with 10(5) HEp-2 cells. After one and two wk, the cells of triplicate control flasks and triplicate flasks containing various dilutions of the production media were enumerated to determine a cell multiplication inhibition (CMI) value. A correlation between the
IFN-beta
content and the CMI effect, however, was not obtained, and it was concluded that other CMI agents, possibly more potent than the
IFN-beta
, were being produced by the stimulated human gingival fibroblasts. Cell protein assays which gave a high ng/cell protein content correlated with
TEM
micrographs which showed clusters of complex lysosomes, primarily in cells cultured in the
IFN-beta
-containing nutrient. However, since commercial
IFN-beta
initiated no such lysosomal response, it was further concluded that the complex lysosomes were due to CMI agent(s) other than
IFN-beta
.
...
PMID:Human gingival fibroblast production of interferon. 621 Mar 14
