Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0276640 (
TEM
)
20,729
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Translin is a DNA/RNA binding protein involved in DNA repair and RNA metabolism. Previously, we had shown that rice
translin
(221 amino acids) exhibits biochemical activities similar to that of the human
translin
protein. Here we report the role of the C-terminal random coil in rice
translin
function by analyzing truncation (after 215
th
residue, Tra - 215) and substitution mutant proteins (Ser216Ala, Lys217Ala, Gln218Ala, Glu219Ala). Circular Dichroism (CD) analysis of Tra-215 showed deviations in comparison to Tra-WT. Truncation abolished the DNA binding activity and octamer formation as evidenced by the absence of ring like structures from
TEM
analysis. CD analysis of the substitution mutant proteins showed that the secondary structure was maintained in all the mutant proteins in comparison to wild type protein. Native PAGE and
TEM
analysis of the substitution mutants showed that Lys217Ala mutation completely abolished the octamer formation as rings and nucleic acid binding. Glu219Ala mutation also affected oligomerization but exhibited marginal RNA binding at higher protein concentrations and interestingly, failed to bind to DNA. However, Ser216Ala and Gln218Ala substitutions did not affect above mentioned activities of
translin
. Our results indicate that the C-terminal residues are one of the determinants of octamer formation in rice
translin
, with lysine at 217
th
position being the most important. Therefore, in conclusion, although the C-terminal residues do not form any defined secondary structure in the
translin
monomer, they are definitely involved in octamer formation and hence important for its molecular function. We have attempted to find the critical residues in
translin
function, which will advance our understanding of
translin
in DNA repair process in general and of rice
translin
in particular.
...
PMID:C-terminal residues of rice translin are essential for octamer formation and nucleic acid binding. 2879 59
