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This study was performed to clarify the movement of calcium in the pancreas of dogs with chronic pancreatitis, using scanning and transmission electron microscopes (SEM, TEM) equipped with X-ray elemental microanalyzers. Eleven adult mongrel dogs underwent incomplete ligation of the pancreatic duct. After the procedure, the levels of pancreatic enzymes in the serum did not change for 9 mo, but the endocrine function was reduced gradually. Of all dogs, 5 revealed pancreatic sclerosis, and 2 at 9 mo had calculi, 2-4 mm in size, in the small pancreatic duct. SEM examination revealed the intralobular fibrosis and irregularity of the pancreatic duct wall, and TEM examination revealed the intralobular fibrosis and irregularity of the pancreatic duct wall, and TEM examination demonstrated the amorphous or crystalloid substances and secreted granules in the acinar lumen or ductule. In elemental analysis spectra using SEM, a high calcium peak was seen in the pancreatic duct wall. In elemental analysis spectra using TEM, a high calcium peak was observed in the amorphous or crystalloid substances, and a high ratio of Ca/K was seen in these substances. Calcium was also detected in the secreted granules or microvilli. These results suggest that there is a process of calculus formation based on the congregation of the intraductular substances containing a large quantity of calcium.
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PMID:Movement of intrapancreatic calcium in dogs with experimental pancreatic lithiasis. With special reference to the process of pancreatic calculus formation. 837 Sep 82

We observed the cilia in pancreatic ducts (intraductal cilia) with scanning and transmission electron microscopy (SEM and TEM), using male WBN/Kob rats (W/K), which are the spontaneously developed chronic pancreatitis models with stasis of pancreatic juice, and male Wistar rats as control. By SEM observations, the lengths of cilia in interlobular ducts of 18-mo-old W/K were demonstrated to elongate markedly. By TEM observations in the controls, cross-sections of the intraductal cilia were demonstrated to present various numbers of microtubules (those with seven, eight, or nine microtubules accounted for 83.3% of all). There was no significant difference between W/K and controls in the number of microtubules in the cross-sections of intraductal cilia: the intraductal cilium core was provided with nine microtubules, which were different from the number of microtubules encountered within the cilium core of other ciliated cell (i.e., bronchial epithelium, and so forth), and their number in the cross-section of intraductal cilium decreased at a distal portion. Though some of their cross-sections revealed deformities of ciliary membranes and disarrangements of microtubular complex, there was no significant difference in their incidence between both rats. These findings suggest that the intraductal cilia have different functions from the ciliated cells' cilia, and W/K has the elongated intraductal cilia without internal structural change.
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PMID:Fine structure of the cilia in the pancreatic duct of WBN/Kob rat. 870 91

The organotin compound di-n-butyltin dichloride (DBTC) is able to induce an acute and later a chronic pancreatitis in rats. In previous papers the authors demonstrated this DBTC pancreatitis as a rat model for an interstitial pancreatitis with tendency to transduction to the chronic form. DBTC is excreted according to its lipophilic nature by liver and bile. Therefore, the bilio-pancreatic main duct is necrotized by the tin-loaded bile. The duct system is blocked by cell debris and later by epithelial proliferations. In the chronic phase, numerous rats develop concrements in the main duct. In the present paper, the authors report about bacterial growth in some bilio-pancreatic concrements. Whereas the electron microscopic detection of tin by energy-dispersive X-ray analysis (EDX) in SEM or electron energy loss spectroscopy (EELS) in TEM was negative in the parenchyma of pancreas and liver, some concrements with bacterial cells were positive for this element. Tin mapping with energy spectroscopic imaging (ESI) in TEM demonstrated the congruency of tin signals and electron-dense particles inside these bacteria and of electron-dense accumulations in the matrix of these concrements. The low content of tin in pancreatic and liver tissue and the higher quantity of tin inside the bacterial contaminated concrements were supported by atomic absorption spectrophotometry (AAS). The paper discusses the long time preservation of tin in the concrements as an action of heavy-metal- accumulating bacteria, which should be classified in the future by bacteriological methods.
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PMID:Electron microscopic detection of tin accumulation in biliopancreatic concrements after induction of chronic pancreatitis in rats by di-n-butyltin dichloride. 1203 97