Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0272170 (
SDS
)
50,377
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
RNA helicases are enzymes important in RNA synthesis, processing, transport, and turnover. Human nucleolar
RNA helicase II/Gu protein
(
RH II/Gu
) was expressed in a baculovirus system. The purified recombinant
RH II/Gu
protein has RNA helicase activity on a 5' tailed ds RNA substrate in vitro. We found that Adriamycin, a widely used anticancer drug, inhibited
RH II/Gu
helicase activity in a dose-dependent manner with an IC(50) of 40 microM. Adriamycin bound to the RNA substrate, and the binding was disrupted by boiling or treatment with 1%
SDS
, suggesting that the binding of Adriamycin to RNA is reversible. Adriamycin was also found by gel electrophoresis to bind to yeast tRNA to form slow-migrating complexes. These results suggest that Adriamycin can inhibit RNA synthesis or processing by binding to RNA substrates.
...
PMID:Adriamycin inhibits human RH II/Gu RNA helicase activity by binding to its substrate. 1060 May 8
