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Query: UMLS:C0271276 (Hudson)
 1,066 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Striped bass (Morone saxatilis) were implanted with beta-estradiol to induce the production of vitellogenin, the egg yolk precursor produced by the liver. Electrophoretic analysis revealed that beta-estradiol caused marked production of a plasma protein of apparent molecular mass 170 kDa. Size exclusion chromatography suggested that the estradiol-induced protein circulated as a dimer. This protein was purified from the plasma of estradiol-treated fish by DEAE-agarose column chromatography and used to induce antibodies in rabbits and goats. Western blots revealed that the antiserum bound to the putative vitellogenin in plasma from estradiol-treated fish and adult females, but not with any proteins in male plasma. Western blot of ovarian extract revealed several smaller immunoreactive protein bands and supported the identity of the purified protein as vitellogenin. A competitive ELISA was developed with sensitivity in a range from 8 to 1000 ng/ml. Plasma concentrations of adult females during their spawning migration ranged from 100 to 600 micrograms/ml. Western blot of mucus extract revealed the presence of a 170-kDa protein in vitellogenic female fish along with several minor bands ranging from 50 to 110 kDa. Positive immunoreactivity was present in the surface mucus of all females and in none of the males collected during a spawning migration in the Hudson River.
Gen Comp Endocrinol 1992 Oct
PMID:Induction by beta-estradiol of vitellogenin in striped bass (Morone saxatilis): characterization and quantification in plasma and mucus. 142 61

The striped bass (Morone saxatilis) is a seasonally breeding, long-lived, anadromous fish of growing economic importance. To describe the apparent activities of growth hormone (GH)- and prolactin (PRL)-producing cells, pituitaries were collected from captive juveniles and from wild adult fish in late spring off the Rhode Island coast during their coastal migration and in the Hudson River during the spawning migration. GH and PRL were separated by reversed-phase HPLC of pituitary extracts from captive adults and characterized as having apparent molecular masses of 23 kDa (GH) and 26 kDa (PRL) by SDS-polyacrylamide gel electrophoresis. Antisera generated in rabbits against synthetic fragments of GH and PRL188 from tilapia were shown by Western blot analysis of reversed-phase HPLC-purified striped bass GH and PRL and of striped bass pituitary extract to be specific for the appropriate hormone and were used to localize GH and PRL cells. GH cells and PRL cells lie in the proximal pars distalis and the rostral pars distalis, respectively. Small clusters of GH- and PRL-immunoreactive cells were found at ectopic sites within the pituitary. There were many intensely labeled GH cells in the pituitaries of juvenile and adult striped bass, whereas the immunoreactivity of GH cells in the pituitaries of spawning fish decreased. PRL cells in juvenile fish kept in fresh water had big, round-nuclei, and were heavily labeled, indicating that they were active. PRL cells in adult fish from seawater were also intensely labeled, but had kidney-shaped nuclei, indicating inactivity. PRL cells in fish from the spawning ground had polymorphic nuclei, appearing as round, indented or kidney-shaped forms, and they were unevenly and lightly labeled, suggesting highly variable stages of activity. No difference was found between males and females. The apparent activities of GH- and PRL-producing cells differ among these life stages, suggesting changing roles for the hormones.
Gen Comp Endocrinol 1994 May
PMID:Growth hormone- and prolactin-producing cells in the pituitary gland of striped bass (Morone saxatilis): immunocytochemical characterization at different life stages. 792 32

We describe approaches to improve the detection of proteins by postharvest alkylation and subsequent radioactive labeling with either [3H]iodoacetamide or 125I. Database protein sequence analysis suggested that cysteine is not suitable for detection of the entire proteome, but that cysteine alkylating reagents can increase the number of proteins able to be detected by iodination chemistry. Proteins were alkylated with beta-(4-hydroxyphenyl)ethyl iodoacetamide, or with 1,5-l-AEDANS (the Hudson Weber reagent). Subsequent iodination using the Iodo-Gen system was found to be most efficient. The enhanced sensitivity obtainable by using these approaches is expected to be sufficient for visualization of the lowest copy number proteins from human cells, such as from clinical samples. However, we argue that significantly improved methods of protein separation will be necessary to resolve the large number of proteins expected to be detectable with this sensitivity.
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PMID:Improved sensitivity proteomics by postharvest alkylation and radioactive labelling of proteins. 1094 35

A cooperative program of seismic refraction profiling was completed in the vicinity of the Puerto Rico Trench by Hudson Laboratories, Woods Hole, Lamont, and Texas A. & M. Profiles completed near the western end of the Trench were analyzed at Hudson Laboratories. Five seismic layers are indicated below the water layer. The thickness/velocity relationships are as follows: 5.1 km of 1.5 km/sec. (water); 1 km of 1.7 km/sec. (sediment); 1.5 km of 3 km/sec. (metamorphics?); 2 km of 5.5 km/sec. (basement); and 2 km of 7.1 km/sec. (high speed basement). Below these, typical Moho velocities of 8.1 km/sec. were measured. Total depth to Moho ranges from 9 to 12 km below sea level, the greatest variation occurring in the basement layers. The least depth was measured 65 miles north of the Puerto Rico Trench.
J Gen Physiol 1962 Mar 01
PMID:Some Seismic Profiles near the Western End of the Puerto Rico Trench. 1987 44