Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0268318 (ICP)
 10,007 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The present study was designed to evaluate the possible use of monoclonal antibodies (mAbs) as diagnostic adjuncts to exfoliative cytology and tissue sections in intraepithelial (CIN) and invasive cervical cancer. Specimens were collected from 42 patients with various degrees of CIN, 15 patients with invasive cancer and two patients with condylomatous changes only. mAb H17, that recognizes a herpes simplex virus protein (ICP) representing a component of the viral ribonucleotide reductase, stained atypical exfoliated cells from 55% of patients with mild dysplasia and 100% of those with more severe lesions. The mean percentage of positive atypical cells increased as a function of the grading of CIN (32.6 +/- 6.3%, 63.5 +/- 2.7%, 67.9 +/- 8.1%, 81.4 +/- 10.1%, and 85.6 +/- 2.0% for mild, moderate, and marked dysplasia, CIS, and invasive cancer, respectively). Only a very small proportion of atypical cells from only two patients stained with a mAb to another herpes simplex virus protein (gA/B). Normal squamous, metaplastic, inflammatory, or koilocytotic cells did not stain with the mAbs. Of the 15 cases examined by cryostatic fresh sections with immunohistochemical techniques, only one case of invasive cancer did not stain with mAb anti-ICP, and all controls were negative. The high specificity and sensitivity of MAbH17 suggests that it may be a useful diagnostic/prognostic marker in CIN.
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PMID:Monoclonal antibody to HSV2 protein as an immunodiagnostic marker in cervical cancer. 282

BglII fragment C mapping between 0.416 and 0.580 map units (mu) on the herpes simplex virus type 2 (HSV-2) genome was used for in vitro translation to identify proteins encoded on this fragment. RNA homologous to the BglII C fragment directs the synthesis of three proteins with approximate molecular weights of 144,000, 52,000 and 27,000. The 27,000 dalton protein is encoded by sequences within the EcoRI/HindIII AE fragment (0.419-0.525 mu) that overlap the immortalizing sequences within BglII C. The 144,000 (144K) and 52,000 dalton proteins are encoded by sequences within the BamHI "e" fragment of HSV-2 DNA (0.535-0.585 mu). The 144K protein is the only species translated in vitro from mRNA hybrid-selected from cells arrested in the "early" (beta) phase of viral protein synthesis. It is precipitated by anti-ICP-10 serum and by monoclonal antibody 48S (previously shown to precipitate the HSV-induced ribonucleotide reductase). The 48S antibody competes with the anti-ICP-10 serum for the 144K protein. Furthermore the in vitro translated 144K protein is structurally similar to ICP-10, an HSV-2-infected cell protein that is antigenically identical to AG-4, the cervical tumor-associated antigen.
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PMID:The cervical tumor-associated antigen (ICP-10/AG-4) is encoded by the transforming region of the genome of herpes simplex virus type 2. 300 Oct 10