Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0268318 (ICP)
 10,007 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

ICP4 of herpes simplex virus is responsible for the activation of viral transcription during infection. It also efficiently activates and represses transcription in vitro depending on the promoter context. The contacts made between ICP4 and the cellular proteins that result in activated transcription have not been identified. The inability of ICP4 to activate transcription with TATA-binding protein in place of TFIID and the requirement for an initiator element for efficient ICP-4-activated transcription suggest that coactivators, such as TBP-associated factors, are involved (B. Gu and N. DeLuca, J. Virol. 68:7953-7965, 1994). In this study we showed that ICP4 activates transcription in vitro using an immunopurified TFIID, indicating that TBP-associated factors may be a sufficient subset of coactivators for ICP4-activated transcription. Similar to results seen in vivo, the presence of the ICP4 C-terminal region (amino acids 774 to 1298) was important for activation in vitro. With epitope-tagged ICP4 molecules in immunoaffinity experiments, it was shown that the C-terminal region was also required for ICP4 to interact with TFIID present in a crude transcription factor fraction. In the same assay, ICP4 was unable to interact with the basal transcription factors, TFIIB, TFIIE, TFIIF, and TFIIH and RNA polymerase II. ICP4 could also interact with TBP, independent of the C-terminal region. However, reflective of the interaction between ICP4 and TFIID, the ICP4 C-terminal region was required for an interaction with FAF250-TBP complexes and with TAF250 alone. Therefore, the interfaces or conformation of TBP mediating the interaction between ICP4 and TBP in solution is probably masked when TBP is bound to TAF250. With a series of mutant ICP4 molecules purified from herpes simplex virus-infected cells, it was shown that ICP4 molecules that can bind DNA and interact with TAF250 could activate transcription. Taken together, these results demonstrate that ICP4 interaction with TFIID involves the TAF250 molecule and the C-terminal region of ICP4 and that this interaction is part of the mechanism by which ICP4 activates transcription.
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PMID:Interaction of the viral activator protein ICP4 with TFIID through TAF250. 864 20

A spectrographic analysis method is described for the determination of B, Si, Cr, Mo, Th and Hf in UF(6) Uranium is separated from UO(2)F(2) using a TBP [tributyl phosphate] extraction-elution resin column technique. The six impurity elements, including B and Si, in the fluorine-containing extraction-elution solution are determined by end-on viewed ICP-AES. The method is simple, rapid and suitable for routine analysis.
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PMID:Determination of B, Si, Cr, Mo, Th and Hf in UF(6) by end-on viewed ICP-AES. 1896 55

A sequential inductively coupled plasma atomic emission spectrometer (ICP-AES) was used to determine Al content in Al-tetrabromophthalate (Al-TBP). Four emission lines of Al (I) at 309.271, 396.152, 308.215 and 394.401 nm were compared. The microwave mineralisation was carried out by using a two-step digestion procedure and HNO(3)-H(2)SO(4). The thermal analysis of Al-TBP and tetrabromophthalic anhydride (TBPA) was performed in the air atmosphere. Statistical tests applied (t-test, F-test) showed no significant differences between the Al results obtained by ICP-AES and thermal method of analysis.
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PMID:Spectrochemical and thermal analysis of Al-tetrabromophthalate and tetrabromophthalic anhydride. A comparison of methods for determination of aluminium. 1896 78

Estimation of impurities in reactor grade uranium is important from the point of view of neutron economy. For chemical separation, ion exchange and solvent extraction techniques have been employed although the latter is generally preferred. Amongst various extractants TBP (tri-n-butyl phosphate), TBP-TOPO (tri-n-octyl phosphine oxide), or TOPO only (in CCl(4), xylene, dodecane) is most often used. New reagents like Cyanex-923 (mixture of 4 tri-alkyl phosphine oxides)/TEHP (tri-ethylhexyl phosphoric acid) are also being used. This communication reports chemical separation of uranium by precipitation using 1,2-diaminocyclohexane NNN'N'-tetra acetic acid (CyDTA)/ammonium hydroxide in presence of 1,10-phenanthroline and estimation of impurities in the filtrate by ICP-AES. Quantitative separation of U, a high spectral interferent in plasma and recovery of impurities have been achieved. Recovery of Cd has been improved by using 1,10-phenanthroline. The method is accurate and precise, offering a relative standard deviation ranging from less than 4% (3.8% for Eu at the 10mug g(-1) level) to 12.9% (for Ce at the 2.5 mug g(-1) level) for all the elements studied.
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PMID:Estimation of trace impurities in reactor-grade uranium using ICP-AES. 1896 50

In this work, a methodology based on a reducing IEF separation in combination with a FASP tryptic digestion able to maintain the integrity of cisplatin-protein complexes has been developed. The method is based on OFFGEL-IEF under conditions provided by the thiol-free reducing agent TBP, which allowed the separation of cisplatin-binding proteins in liquid fractions. The FASP procedure is applied as an intermediate stage between the IEF separation and MS analysis where the proteins are retained and concentrated in a commercially available ultrafiltration device. The filter unit acts as a proteomic reactor for detergent removal, buffer exchange, chemical modification (reduction and alkylation) and protein digestion. Finally, purified peptides are recovered by centrifugation. This procedure provides efficiencies comparable to standard in-solution digestion and the risk of platinum-complexes loss is minimized due to the fact that reagents employed along the process are subsequently eliminated before the following step. The stability of platinum-protein complexes under the FASP tryptic digestion, either using TBP or DTT as reducing agents, was maintained, allowing the identification of several platinum-containing peptides from cisplatin-HSA. This methodology was applied to the separation of platinum-enriched protein fractions obtained by SEC-ICP-MS in a kidney tissue extract from a rat treated with cisplatin, followed by further identification by nLC-ESI-LTQ-MS/MS after FASP tryptic digestion of selected platinum-containing liquid fractions.
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PMID:Combining TBP-based rOFFGEL-IEF with FASP and nLC-ESI-LTQ-MS/MS for the analysis of cisplatin-binding proteins in rat kidney. 2446 93