Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0268318 (ICP)
 10,007 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Vanadate-dependent peroxidase A.n.I, the main isoenzyme (M(r) = 100 kDa) from the seaweed, Ascophyllum nodosum, contains 2 V per enzyme molecule (as shown by ICP-MS metal analysis) after complete reconstitution with vanadate (V), possibly distributed in a 1:1 ratio between the surface and active site. VO2+ is only weakly associated to the surface of A.n.I. There is no transport channel for VO2+. The EPR spectrum of the reduced holoenzyme is anisotropic (axial) already at room temperature, with EPR parameters similar to those of VO2+ complexes of small model peptides such as Ala-His, Gly-Tyr, Gly-Ser, Gly-Glu, Ser-Gly and Phe-Glu. The complex formation between Ala-His and H2VO4- in water has also been investigated (by 51V NMR); the formation constant at pH 7.2 amounts to 266(28) M-1.
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PMID:(Model) studies on vanadate-dependent bromo/iodoperoxidase from Ascophyllum nodosum. VO2+ is not incorporated into the active site. 131 46

Two cases of skullbase-penetrating injuries caused by umbrella tips are reported. Case 1: 24-year-old male. Admitted with disturbance of consciousness, left hemiparesis, nasal bleeding, and laceration of left lower eyelid because of having been stabbed by an umbrella tip. Pupils and fundi revealed no definite findings. Plain skull X-ray showed turbid ethmoid sinus and fracture of planum sphenoidale. Cranial CT showed right putaminal hematoma with intraventricular hemorrhage and pneumocephalus. Increased ICP necessitated surgery two days after the injury. Dural laceration of planum sphnoidale, laceration of left optic nerve, right rectal gyrus contusion and rebleeding from the right lenticulostriate branch were observed. Dural plasty and removal of hematoma with external decompression were carried out. He had a good postoperative course, but left visual loss and left hemiparesis remained. Case 2: 29-year-old male. Admitted with excoriation of his right nostril because of having been stabbed by an umbrella tip, severe headache, and nasal discharge. Oculomotor palsy was observed as well as CSF rhinorrhea and meningeal irritability. Plain skull X-ray showed niveau in sphenoidal sinus, pneumocephalus, and fracture of sella turcica. His complaint disappeared after conservative therapy. We reviewed the literature and found only 4 similar cases. The skullbase, because of its anatomical character, is likely to be penetrated in orbital and periorbital injury caused by umbrella tips. Cases which include disturbance of consciousness have a poor prognosis. We hope the fact that umbrella tips can easily become life-threatening objects will come to the attention of the general public so that similar cases may be avoided.
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PMID:[Skullbase-penetrating injuries caused by umbrella tips: case reports]. 218 93

The preparation and characterisation of a Ru-modified derivative of the [2Fe-2S] ferredoxin FdI component of A. variabilis by attachment of Ru(NH3)5 to a surface histidine has been carried out. Metal analyses by ICP gave an Fe/Ru ratio of 1.97:1. From NMR the attachment is confirmed as a modification at His-16 and not His-92. No Ru-modification of the [2Fe-2S] FdI component of spinach, which has His-92 but no His-16, was observed. Histidine pKa values have been determined and anomalously low values for A. variabilis (5.3) and spinach (< 5.0) His-92 residues are noted, whereas His-16 gives a pKa of 6.95. Pulse radiolysis techniques with e-aq as reductant have been used to generate the metastable Fe(II)Fe(III)Ru(III) state from Fe(III)2Ru(III), k = 8.8 x 10(10) M-1 s-1. Intermolecular, k(inter) = 4.3 x 10(6) M-1 s-1 at approx. 19 degrees C, but no intramolecular electron-transfer (ET) Fe(II)Fe(III) to Ru(III) contribution was observed with Fe(III)2Ru(III) in the range 7-30 microM. The driving force for ET is approx. 500 mV, and the edge to edge distance from the C gamma of His-16 to the nearest cysteinyl S-atom attached to the Fe2S2 cluster is 16.1 A. Using the Beratan-Onuchic pathway approach, the most favourable ET intramolecular route consists of 20 covalent and 3 H-bonds, with a total distance from the S of Cys-49 to the C gamma of His-16 of 37.1 A. We note that the reduced Fe is on the remote side of the [2Fe-2S] cluster from the site of Ru-attachment.
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PMID:Pulse radiolysis and related studies on the Ru-modified His 16 derivative of Anabaena variabilis [2Fe-2S] ferredoxin. 837 14

Absorption, CD, fluorescence, and ICP(AES) methods were used to evaluate the interaction of [Rh2(OAc)2(bpy)2(H2O)2](OAc)2 with human serum albumin (HSA). The rhodium complex reacts easily with HSA; the Rh atoms are coordinated to protein via the imidazole rings of His residues. When the protein was incubated for 24 h at 37 degrees C, the amount of rhodium was found to be approximately 7 mol per mol of HSA. Analysis of CD spectra showed the decreasing helix content to be about 15% in the metal-bound HSA. The relative fluorescence intensity of HSA bound with rhodium decreased to 20% of that of the native state, suggesting that perturbation around the Trp-214 residue took place. This was confirmed by the destruction of the warfarin binding site. The rhodium binding weakens the interaction of HSA with other molecules like heme or bilirubin.
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PMID:Studies on the interaction between human serum albumin and [Rh2(OAc)2(bpy)2(H2O)2](OAc)2. 913 Mar 93

Mutant herpes simplex virus type 1 (HSV-1) viruses were constructed to characterize the roles of the conserved histidine residues (H61 and H148) of HSV-1 protease in the regulation of catalytic activity and virus maturation. Viruses containing mutations at H61 (H61V-V711, H61Y-V715, and H61A-V730) were unable to grow on Vero cells. These mutant viruses could process neither Pra to N0 nor ICP-35cd to ICP-35ef. Transmission electron microscopy studies of H61A-V730-infected Vero cells indicated that capsid maturation is arrested at a state characterized by the predominance of large symmetrical arrays of B capsids within the nucleus. Two mutations at H148 (in viruses H148A-V712 and H148E-V728) gave rise to mutant viruses that grew with a small-plaque phenotype; one of the viruses, H148E-V728, was particularly attenuated when grown at a low multiplicity of infection. The rate of processing of Pra to N0 in infected Vero cells increased in the order H148A-V712 < H148E-V728 < parental strain HSV-1-V731. The observation that H148A-V712 processes Pra to N0 and ICP-35cd to ICP-35ef, whereas H61A does not, establishes H61 as the catalytically essential conserved His assuming that HSV-1 protease, like other serine proteases, utilizes an active-site histidine residue in catalysis. Two of the mutations at H148 (viruses H148K-V729 and H148Y-V716) produced nonviable viruses. H148K-V729 processed neither Pra to N0 nor ICP-35cd to ICP-35ef, whereas H148Y-V716 processed Pra to N0 but did not process ICP-35cd to ICP-35ef. The range of phenotypes observed with the H148 mutant viruses suggests that residue 148 of the HSV-1 protease is a determinant of virus growth rate and viability because of its effects on the activity of the protease and/or the role of the protease domain in capsid assembly and DNA packaging.
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PMID:Alterations in catalytic activity and virus maturation produced by mutation of the conserved histidine residues of herpes simplex virus type 1 protease. 934 15

The abnormal form of the prion protein (PrP) is believed to be responsible for the transmissible spongiform encephalopathies. A peptide encompassing residues 106-126 of human PrP (PrP106-126) is neurotoxic in vitro due its adoption of an amyloidogenic fibril structure. The Alzheimer's disease amyloid beta peptide (Abeta) also undergoes fibrillogenesis to become neurotoxic. Abeta aggregation and toxicity is highly sensitive to copper, zinc, or iron ions. We show that PrP106-126 aggregation, as assessed by turbidometry, is abolished in Chelex-100-treated buffer. ICP-MS analysis showed that the Chelex-100 treatment had reduced Cu(2+) and Zn(2+) levels approximately 3-fold. Restoring Cu(2+) and Zn(2+) to their original levels restored aggregation. Circular dichroism showed that the Chelex-100 treatment reduced the aggregated beta-sheet content of the peptide. Electron paramagnetic resonance spectroscopy identified a 2N1S1O coordination to the Cu(2+) atom, suggesting histidine 111 and methionine 109 or 112 are involved. Nuclear magnetic resonance confirmed Cu(2+) and Zn(2+) binding to His-111 and weaker binding to Met-112. An N-terminally acetylated PrP106-126 peptide did not bind Cu(2+), implicating the free amino group in metal binding. Mutagenesis of either His-111, Met-109, or Met-112 abolished PrP106-126 neurotoxicity and its ability to form fibrils. Therefore, Cu(2+) and/or Zn(2+) binding is critical for PrP106-126 aggregation and neurotoxicity.
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PMID:Copper and zinc binding modulates the aggregation and neurotoxic properties of the prion peptide PrP106-126. 1143 76

Decompressive craniectomy sometimes causes neurological deficits known as 'the syndrome of the sinking skin flap' or 'the symptom of the trephined'. These disorders can be corrected with cranioplasty, but there is no consensus on appropriate treatments. We report a case of successful correction of traumatic hydrocephalus following craniotomy. A 50-year-old man was admitted to our hospital with disturbance of consciousness after a head injury. Decompressive craniectomy was performed for a right acute subdural hematoma. His consciousness recovered after the operation, but then deteriorated gradually and left hemiparesis occurred. CT scan revealed midline shift from right to left. These symptoms and CT findings were not improved after cranioplasty, but were improved with removal of the CSF from the adhered subarachnoid space. The diagnosis was traumatic hydrocephalus, and a cisternoperitoneal shunt was subsequently placed. We report this case to emphasize the necessity for study of CSF circulation, as well as the importance of examination of CBF and ICP after craniectomy.
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PMID:[A case of the traumatic hydrocephalus after large craniectomy for acute subdural hematoma]. 1261 57

Premature craniosynostosis is caused by a premature closure of single or multiple sutures of the cranial vault. It may result not only in aesthetic but also in functional disorders with an occasional ICP increase leading to a dramatic loss of vision. In the 1970s,Paul Tessier published his craniofacial principles for the primary treatment of craniosynostosis. His approach has gained wide acceptance over the previously described craniectomies and bone flaps, especially because of the significant benefits with an acceptably low level of morbidity. The following paper reviews the different kinds of craniosynostoses, their specific clinical manifestations, the necessary diagnostic examinations and the specific surgical procedures and operative results.
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PMID:[Diagnosis and therapy of syndromic and non-syndromic craniosynostosis]. 1262 47

A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP-MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material.
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PMID:Dialysis-Chelex method for determination of exchangeable copper in human plasma. 1499 Dec 16

Direct interaction of Cu2Zn2SOD with inorganic metal compound (CoCl2) and organic metal compound [Co(II)(His)n] was studied by ICP, VIS, NMR and measurement of enzyme activity. It has been found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2Zn2SOD) with external added CoCl2, Co(His)n. As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives were produced and the catalytic activity of enzyme were affected. Moreover, the interaction of Co(II)(His)n with Cu2Zn2SOD is stronger and quicker, than that of CoCl2 with this enzyme, as well as the Co(II) from Co(II)(His)n is easier to enter the active center of enzyme.
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PMID:[Study on the interaction of Cu2Zn2SOD with CoCl2, Co(His)n by spectral and magnetic resonance analysis]. 1581 41


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