Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0267964 (PAA)
 2,561 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Poly(acrylic acid) (PAA) brushes are well-known to interact with proteins in an ionic strength-dependent way. Moreover, they provide a native-like environment that largely maintains the secondary structure and biological activity of adsorbed proteins. Recently, it has been shown that the application of high pressure can lead to a reduced protein adsorption at a PAA brush in the case of a positively charged protein. Here, we analyze the effect of pressure on the interactions between a protein and a PAA brush in more detail. We use calmodulin as model protein that has a negative net charge at neutral pH-value and determine the degree of adsorption at a planar PAA brush applying total internal reflection fluorescence (TIRF) spectroscopy. Remarkably, the degree of calmodulin adsorption at a PAA brush is increasing with increasing pressure, when the protein is negatively charged. However, at low pH-value, where calmodulin is positively charged, high pressure leads to a partial desorption of the protein. Moreover, in the presence of trifluoperazine, which binds to calmodulin as a ligand, the pressure effect is diminished. The results of this study indicate that protein adsorption at a PAA brush at the "wrong" side of the isoelectric point, i.e. under net electrostatic repulsion, can involve a volume reduction that is favored under high pressure. It is suggested that this volume reduction is related to a hydration of counterions that are released from the PAA chains and the protein surface. In contrast, at pH-values close to the isoelectric point, the obtained data are consistent with a charge regulation mechanism that involves a volume increase. Thus, the application of high pressure in combination with pH-variation, as carried out in this study, provides the volume changes of adsorption that need to be consistent with any proposed mechanism of protein interaction with a PAA brush.
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PMID:Interaction of calmodulin with poly(acrylic acid) brushes: Effects of high pressure, pH-value and ligand binding. 3007 5