Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0267964 (PAA)
 2,561 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spleen cells from male (CBA/N x DBA/2) F1 hybrid mice do not significantly respond to in vitro stimulation by trinitrophenyl-conjugated polyacrylamide beads (TNP-PAA), whereas the same antigen elicits high PFC responses in female F1 hybrid cells. Therefore, this antigen could be classified as a T-independent type 2 (TI-2) antigen. When male spleen cells were co-stimulated by TNP-PAA and TI type 1 antigen, either LPS or Brucella abortus, they produced vigorous anti-TNP responses. A similar increase of the in vitro responsiveness of male F1 hybrid spleen cells to TNP-PAA antigen was provoked by the addition of supernatants from P 388-D1 cells stimulated by muramyl-dipeptide (MDP) mainly containing interleukin-1 (IL-1) or supernatants from phorbol 12-myristate 13-acetate (PMA)-stimulated EL-4 cells that contained T-cell factors. The PFC response to another TI-2 antigen, TNP-Ficoll, was also significantly enhanced after co-stimulation by P 388-D1 supernatants. The response to TI-2 antigens being macrophage dependent, the influence of supernatants of peritoneal macrophages from male and female F1 hybrids incubated with TNP-Ficoll on the PFC response of normal DBA/2 mouse spleen cells to sheep erythrocytes was assessed. It was found that macrophage supernatants from female hybrids regularly increased by more than two times this anti-SRBC PFC response, whereas macrophage supernatants from male F1 hybrids did not. Moreover, in a specific proliferation test measuring IL-1 activity, when macrophage supernatants from female F1 produced a 13-fold increase of thymidine incorporation, supernatants from male F1 only produced a three-fold increase. It is concluded that, in addition to the known defects of B cells from Xid mice, their macrophages are also defective.
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PMID:Xid mouse lymphocytes respond to TI-2 antigens when co-stimulated by TI-1 antigens or lymphokines. 329 78

Growth medium simulating the phagolysosomal environment in which Yersinia pestis resides during its intracellular growth in vivo was made by acidification of Ca2+-deficient medium. When used for cultivation of Y. pestis EV-76 (pLCR+;pPst+;pFra+) and its isogenic derivatives--KM-217 (pLCR+;pPst-;pFra-) and KM-218 (pLCR-;Ppst-; pFra-)--this medium permitted survival and proliferation of viable bacteria without any growth restriction. Moreover, a correlation between the pH of growth medium and bacterial yield was established. Acidification completely inhibited fibrinolytic (pla protease) activity (PAA) of Y. pestis carrying pPst and allowed synthesis of specific outer-membrane proteins (Yops) without any degradation by the pla protease. Comparison of whole-cell lysates of the strains tested in PAAG-SDS showed that, in addition to previously described Yops, Y. pestis synthesised new acidic proteins which appeared only under acidic conditions and were encoded by pLCR or chromosomally. Some changes in O-specific polysaccharide chains of Y. pestis LPS that were dependent on cultivation temperature and pH of the medium were also demonstrated.
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PMID:Expression of acid-stable proteins and modified lipopolysaccharide of Yersinia pestis in acidic growth medium. 1169 95