Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0267964 (PAA)
 2,561 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In this work we studied the alteration of Z-line alpha-actinin in striated skeletal muscle, under conditions of experimental ischemia, by immunohistochemical techniques. alpha-Actinin was extracted and purified from rabbit striated skeletal muscle and used, as an antigen, for the production of antibodies, in sheep. Electrophoresis was performed on PAA sla-gel and the Ag-Ab reaction test on agarose slab-gel (immunodiffusion). Antibodies were used for the indirect immunoperoxidase technique on semithin sections of rabbit striated skeletal muscle, included in in Araldehyde, and observed with phase-contrast microscopy. alpha-Actinin from ischemic muscle because of stenosis of the iliac artery, undergoes a progressive alteration, from the third hour of ischemia, going out of its usual sites. From the fifth to the eighth hour it gradually disappears until it leaves rare and residual points of positive reaction only in correspondence to the Z-lines.
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PMID:[Morphological changes alpha-actinin in skeletal striated muscle in the rabbit in experimental ischemia]. 676 19

In this work we study alpha-actinin sites in rabbit fibroblasts in culture. For this purpose we extracted alpha-actinin from rabbit striated skeletal muscle and produced the relative antibodies in sheep. Electrophoresis was performed, on PAA slab-gel and the immunodiffusion test on agarose slab-gel. Antibodies were used for direct and indirect immunofluorescence techniques by TRIC and FITC. We noticed that in young fibroblasts alpha-actinin is concentrated in perinuclear regions, while in adult fibroblasts it is scattered, more or less evenly in the cytoplasm and above all on the plates which link the cell membrane to the substractum. Both the direct immunofluorescence method with antibodies anti-alpha-actinin, marked by TRITC, and the indirect one with sheep IgG, marked by FITC, gave identical results.
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PMID:[Dynamic aspects of alpha-actinin in cultured rabbit fibroblasts studied with immunofluorescence]. 676 20