Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0267964 (
PAA
)
2,561
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Sphingomonas sp. KT-1 hydrolyzes poly(aspartic acid) (
PAA
) containing alpha- and beta-amide units and has at least two different types of
PAA
hydrolases. The
PAA
hydrolase-1 hydrolyzes selectively beta-beta amide units in
PAA
. Molecular cloning of
PAA
hydrolase-1 from Sphingomonas sp. KT-1 has been carried out to characterize its gene products. Genetic analysis shows that the deduced amino acid sequence of
PAA
hydrolase-1 has a similarity with those of the catalytic domain of poly(3-hydroxybutyric acid) (
PHB
) depolymerases from Alcaligenes faecalis AE122 and Pseudomonas lemoignei. Site-specific mutation analysis indicates that (176)Ser is a part of a strictly conserved pentapeptide sequence (Gly-Xaa-Ser-Xaa-Gly), which is the lipase box, and plays as an active residue.
...
PMID:Genetic analysis and characterization of poly(aspartic acid) hydrolase-1 from Sphingomonas sp. KT-1. 1252 51
