Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0267964 (PAA)
 2,561 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The quantity of urinary proteins and their molecular weight composition was analyzed in different experimental glomerulopathies using the SDS-PAA-electrophoresis. Masugi nephritis, heterologous and autologous immune complex nephritis as well as D-penicillamine induced glomerulonephritis were studied in rabbits, guinea pigs and rats. The procedure allows (1) to distinguish physiological from low grade glomerular proteinuria by their respective characteristic patterns in early disease stages (2) to follow up the disease course of individual animals without sacrifice and (3) to discriminate species specificity of physiological urinary proteins. It is recommended to use this technique of urinary protein analysis in experimental conditions, where mild glomerular damage is expected.
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PMID:Molecular weight analysis of proteinuria in experimental glomerulopathies. 13 32

The SDS polyacrylamide gelelectrophoresis (SDS-PAA) as used in this study has proven to be an excellent tool to differentiate urinary proteins qualitatively and quantitatively, since the proteins are differentiated exclusively according to their molecular radius. Selectivity was estimated by the ratio transferrin:IgG. Some of the proteins were identified by specific antisera. For clinical use SDS-PAA may distinguish: chronic glomerulonephritis from chronic pyelonephritis; the different diabetic nephropathies; some cases of minimal change nephritis from proliferative and degenerative glomerular diseases; the uncomplicated posttransplantation course from (interstitial) rejection crises and from glomerular diseases (recurrent GN, glomerular rejection disease), and the persisting small glomerular proteinuria after acute glomerulonephritis from proteinurias becoming physiological.
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PMID:Discelectrophoretic molecualr weight analysis of urinary proteins. A contribution to the clinical diagnostic differentiation and the pathophysiology of proteinuria. 123 87