Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0265264 (
HOS
)
1,119
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Kruppel-like factor 6
(
KLF6
) is a tumor suppressor gene frequently downregulated in a number of human cancers, including osteosarcoma. However, the role of
KLF6
in osteosarcoma remains unclear. This study was aimed at investigating the effects of
KLF6
on osteosarcoma cell biological behavior. First, the expression of
KLF6
in osteosarcoma cell lines (MG63, SaOS-2, U2OS, and
HOS
) and a human osteoblastic cell line (hFOB1.19) was detected by Western blotting. Results showed that
KLF6
displayed a significant downregulation in osteosarcoma cell lines (MG63, SaOS-2, U2OS, and
HOS
) compared with human osteoblastic cell line (hFOB1.19). To investigate the role of
KLF6
in osteosarcoma cell proliferation, apoptosis, and invasion, we generated human osteosarcoma MG63 cells in which
KLF6
was either overexpressed or depleted. The MG63 cell viability, cycle, apoptosis, and invasive ability were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide staining, propidium iodide (PI) staining, Annexin-V-FITC/PI double staining, and Transwell invasion experiment, respectively. Results showed that the viability, proliferation, and invasive abilities were suppressed, and the apoptosis was enhanced in MG63 cells with overexpression of
KLF6
. The viability, proliferation, and invasive abilities were improved, and the apoptosis was inhibited in MG63 cells with knockdown of
KLF6
. At the same time, these molecules, including p21, bcl-2, and MMP-9, associated with the events about cell cycle, apoptosis, and invasion, were detected. Results showed that the expressions of bcl-2 and MMP-9 were downregulated, and the expressions of p21 were upregulated in the MG-63 cells with overexpression of
KLF6
. Taken together, our results suggested that
KLF6
could inhibit proliferation and invasion, and facilitate apoptosis of osteosarcoma cells, which might be a potential target for the treatment of osteosarcoma.
...
PMID:Effects of Kruppel-like factor 6 on osteosarcoma cell biological behavior. 2332 24
