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Query: UMLS:C0265264 (HOS)
 1,119 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To identify the cellular receptors and other cell surface molecules playing essential roles in the transmission of human T-cell leukemia virus type 1 (HTLV-1), we have been isolating monoclonal antibodies (mAbs) that are capable of inhibiting HTLV-1-induced syncytium formation. In the present study, we isolated two mAbs, H11 (IgM) and H14 (IgG1), inhibitory to syncytium formation in the coculture of TOM-1 or C91/PL (both HTLV-1-positive human T-cell lines) and MOLT-4/8 (HTLV-1-negative human T-cell line) by immunizing the membrane fraction of human osteosarcoma line HOS. By immunoprecipitation and immunoblotting, H11 and H14 were found to be specific for MHC class I heavy chain and beta 2-microglobulin (beta 2 M), respectively. Among the four commercially obtained mAbs, two mAbs for MHC class I antigen and two mAbs to beta 2 M, one mAb to MHC class I antigen and one mAb to beta 2 M were also found to be inhibitory to the syncytium formation. The functional comparison of these mAbs revealed that the syncytium-inhibitory mAbs induced strong homotypic cell adhesion particularly in the HTLV-1-positive T-cell lines. This cell adhesion was dependent on temperature, energy metabolism, and microfilament function but not on the activity of protein kinase C or divalent cations. These results suggest a novel type of LFA-1-independent cell adhesion induced by signal transduction via MHC class I antigen.
Cell Immunol 1992 Sep
PMID:Induction of strong homotypic adhesion in human T cell lines positive with human T-cell leukemia virus type 1 by monoclonal antibodies to MHC class I and beta 2-microglobulin. 138 Aug 95

A human osteosarcoma cell line, HOS TE85 cells, and a mouse osteoblastic cell line, MC3T3-E1 cells, were cultured for 3 days in a medium containing various concentrations of menaquinone-4 (vitamin K2). As a result, the proliferation of HOS cells was suppressed by vitamin K2 in a dose dependent manner up to 56% of control by 10(-7)M of vitamin K2 and that of MC3T3-E1 cells was suppressed to 84% of control by 10(-6)M of vitamin K2. Vitamin K2 increased alkaline phosphatase activity in both kinds of cells. Warfarin counteracted the effect of vitamin K2 on osteoblastic cell proliferation. Our results show that vitamin K2 modulates proliferation and function of osteoblastic cells by some mechanisms including gamma-carboxylation system.
Biochem Biophys Res Commun 1992 Sep 16
PMID:Vitamin K2 modulates proliferation and function of osteoblastic cells in vitro. 153 Jun 37

Although frozen semen is now being used clinically, the fertilizing function of thawed semen has not yet been evaluated adequately. The changes in frozen sperm functions were evaluated and the changes in regard to the fertilization phenomenon were also investigated. The collected semen was evaluated by the following function tests, 1) Bovine cervical Mucus Penetration test (BMP test), 2) Zona-free hamster egg Sperm Penetration Test (ZSPT), 3) Hypoosmotic Swelling test (HOS test), 4) Triple stain technique (AR test), 5) Semen Auto Analyzer. The freezing medium was KS-II solution with a program freezer (CRYO-10). The results were as follows: 1) In the BMP test, the frozen normospermic group maintained fertilizing capacity. 2) There was no significant difference between fresh and frozen semen in the penetration rate of ZSPT except in the oligospermic group. 3) In the HOS test, there was no difference between fresh and frozen specimens in the number of swollen sperms which endured freezing and maintained the sperm membrane integrity. 4) There was a tendency to compare to that of fresh semen the increase in AR in frozen semen, but it was not significant. 5) There were low values after thawing except for LHD, but there still remained fertilizability after freezing. In conclusion, there was no reduction in fertilizing capacity following freezing through out the sperm function tests.
Nihon Sanka Fujinka Gakkai Zasshi 1991 Sep
PMID:[Fertilizing capacity of fresh and frozen spermatozoa]. 191 85

The DNA probes met and pJ3.11 are derived from loci on chromosome seven that are closely linked to, and probably flanking, the gene mutation causing cystic fibrosis (CF). We have shown that mitotic chromosomes from the cell line MNNG-HOS, which contains an activated met oncogene, can induce morphological transformation of mouse NIH-3T3 cells. Southern analysis of isolated transfectant cell lines with cloned dispersed repetitive human DNA sequences as probes demonstrated that several lines of transformed NIH 3T3 cells had stabley incorporated large segments of chromosome seven DNA. Southern blot analysis also demonstrated the presence of met, pJ3.11 and several other single copy sequences that had been previously localised to chromosome 7 within the transgenomes. In this way a further four genetic markers were shown to be physically linked to met, and thus to CF. These probes may prove useful in confirming the order of loci around CF and in the prenatal diagnosis of this common autosomal recessive disease.
Nucleic Acids Res 1986 Sep 25
PMID:Chromosome mediated gene transfer of six DNA markers linked to the cystic fibrosis locus on human chromosome seven. 376 3

An autosomal dominant syndrome is described in 26 members of six generations in a single family. Distal extremity malformations are characteristic and superficially resemble those of arthrogryposis, chondroectodermal dysplasia, Cornelia de Lange syndrome, Faconi's anemia or Holt-Oram syndrome. There is an absence of spinal deformity, and females of the disorder have duplication of the genital tract.
J Manipulative Physiol Ther 1986 Sep
PMID:The hand-foot-uterus syndrome: a case study. 377 65

We report on the presence of progesterone receptor and its mRNA in a human osteoblast-like cell line (HOS TE85) using recombinant DNA methods, saturation analysis, specific immunoprecipitation with an anti-progesterone receptor antibody, and Northern blot analysis. These osteoblast-like cells were also found to be responsive to progesterone in a dose-dependent fashion through an augmentation in cellular alkaline phosphatase activity. These results support the presence of progesterone receptors in bone cells and suggest a direct effort of progesterone and/or antiprogestins on osteoblast cell function.
Biochem Biophys Res Commun 1993 Sep 15
PMID:Evidence for progesterone receptors in human osteoblast-like cells. 769 May 54

Holt-Oram syndrome is an autosomal dominant disorder with congenital heart defects and skeletal malformations of the upper extremities. A patient with a deletion of 14q23-24 and Holt-Oram syndrome has been described. In this report, however, genetic linkage to the 14q23-24 region is excluded in a multigeneration family with five available individuals affected with Holt-Oram syndrome. Familial Holt-Oram syndrome might be different from the syndrome with the 14q23-24 deletion.
Clin Genet 1994 Sep
PMID:Exclusion of linkage to 14q23-24 in a family with Holt-Oram syndrome. 782 Sep 41

Expression of estrogen receptor (ER) was studied in MC3T3-E1 cells (mouse osteoblastic cell line), HOS TE85 cells (human osteosarcoma cell line), and primary osteoblastic cells derived from mouse calvaria with immunohistochemical techniques. The staining of ER was readily detectable in MC3T3-E1 cells, HOS TE85 cells, and primary osteoblastic cells by using a monoclonal anti-ER antibody that recognizes the DNA binding domain of ER. The immunoreactivity was distributed in the cytoplasm as well as in the nuclei. 17 beta-Estradiol (10(-8) M) did not alter this staining pattern. The expression of ER was confirmed by Northern blot analysis using rat ER cDNA probe, which revealed a 6.5 kb band in MC3T3-E1 cells and a 6.2 kb band in HOS TE85 cells. The mRNA level of ER was not altered by 17 beta-estradiol (10(-8) M). The immunohistochemical studies showed that ER was not detectable in all cells but in a small population of each cell type. This study is the first report to demonstrate the presence of ER immunohistochemically, and our results suggest the heterogeneity of ER expression among osteoblastic cells.
J Bone Miner Res 1993 Sep
PMID:Immunohistochemical detection and northern blot analysis of estrogen receptor in osteoblastic cells. 823 80

The alteration of main disaccharide units in the skin of hairless mice (HOS:Hr 1) after chronic and repeated ultraviolet light (UV) radiation was investigated using high performance liquid chromatography after labeling with 1-phenyl-3-methyl-5-pyrazolone. The total amount of main disaccharide units increased by UVA irradiation at the 24th and 36th weeks in comparison with the control. At the 36th week UVA significantly increased hyaluronic acid-derived delta Di-HA (HA). Dermatan sulfate-derived delta Di 4S (DS) and chondroitin sulfate-derived delta Di-4S (CS) increased at the 36th week, although not statistically significant. The total amount of main disaccharide units was increased significantly by UVB irradiation at the 24th week as compared with the control. Concerning the compositional change in main disaccharide units after a 36-week repeated exposure, the decrease in delta Di-HA(HA) and the increase in delta Di-4S (DS) were found in the order of control, UVA- and UVB-irradiated groups. These results, for the first time, indicate the precise alterations of glycosaminoglycans, both in the total amount and in the composition, confirming the previous histochemical findings. This disaccharide analysis should provide a useful method to examine the biochemical changes of skin glycosaminoglycans in photoaging.
J Dermatol Sci 1995 Sep
PMID:Disaccharide analysis of the skin glycosaminoglycans in chronically ultraviolet light-irradiated hairless mice. 853 12

The recent identification of coreceptors that mediate efficient entry of human immunodeficiency virus type 1 (HIV-1) suggests new therapeutic and preventive strategies. We analyzed simian immunodeficiency virus (SIV) entry cofactors to investigate whether the macaque SIV model can be used as an experimental model to evaluate these strategies. Similar to primary HIV-1 isolates, a well-characterized molecular clone, SIVmac239, which replicates poorly but efficiently enters into rhesus alveolar macrophages and an envelope variant, SIVmac239/316Env, with an approximately 1,000-fold-higher replicative capacity in macrophages used the beta-chemokine receptor CCR5 for efficient entry. The transmembrane portion of 316Env allowed low-level entry into cells expressing CCR1, CCR2B, and CCR3. A single amino acid substitution in the V3 loop of SIVmac239/316Env, 321P-->S, impaired the ability to enter into the T-B hybrid cell line CEMx174 but had relatively little effect on entry into primary cells and HOS.CD4 cells expressing CCR5. Although CEMx174 cells do not express CCR5, most SIVmac variants entered this hybrid cell line efficiently but did not enter the parental T-cell line CEM. It seems likely that CEMx174 cells express an as-yet-unidentified, perhaps B-cell-derived cofactor which allows efficient entry of SIVmac.
J Virol 1997 Sep
PMID:Simian immunodeficiency virus variants with differential T-cell and macrophage tropism use CCR5 and an unidentified cofactor expressed in CEMx174 cells for efficient entry. 926 70


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