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Query: UMLS:C0242706 (hyperoxia)
 5,219 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Apoptosis in the central nervous system (in contrast to necrosis) is an endogenous cell suicide mechanism triggered in response to biological factors and genotoxic stimuli often resulting from oxidative stress. Excessive neural apoptosis may result in longterm brain dysfunction. A significant proportion of prematurely born infants are exposed to high oxygen and nutritional regimens deficient in antioxidant precursors. Such infants frequently display cognitive deficits when studied in later childhood. Studies in cell culture have characterized a close relationship between oxidative stress, glutathione availability and cell death. Here, we assessed this relationship in rat brain, as a model approximation of the situation that occurs in human infants. Two day old rats were exposed to an atmosphere of 95% oxygen and treated with buthionine sulfoximine (BSO), a glutathione synthesis inhibitor. Control groups consisted of rat-pups kept in air, air plus BSO, or oxygen alone. At the end of 5 days of treatment, brains were harvested, dissected and nerve growth factor protein (NGF), glutathione, and extent of apoptosis were measured. Hyperoxia induced a decrease in NGF protein while BSO induced a decrease in glutathione concentrations. Animals treated with both hyperoxia and BSO had a dramatic increase in the extent of brain apoptosis detected. We conclude from these studies that the brains of animals exposed to both oxidative stress and limited antioxidant protection are liable to pro-apoptotic changes. Increased cell death via apoptosis reflecting changes in neurotrophin and glutathione homeostasis may represent the mechanism responsible for the induction of the longterm cognitive deficits observed in some preterm infants.
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PMID:Induction of apoptosis in the CNS during development by the combination of hyperoxia and inhibition of glutathione synthesis. 984 Jul 39

Liposome-encapsulated hemoglobin (LEH) has been developed as a blood substitute. In spite of its size (1/30 - 1/40 of erythrocytes), LEH has an oxygen-carrying capacity comparable to erythrocytes. Thus, LEH is expected to carry oxygen into vital organs via collateral routes during ischemia induced by vascular embolism. In the present study, we examined the therapeutic effects of LEH on behavioral impairments in rats after four-vessel occlusion (4VO) for 30 min. In the open-field test, locomotor activity in 4VO rats did not alter 7 days after ischemia. However, in the contextual fear conditioning (CFC) test, the freezing rate was significantly decreased in 4VO rats, although no behavioral changes in the Y-maze test and elevated plus-maze test were observed. Phosphorylation of the cyclic AMP response element-binding protein (CREB) in the hippocampal CA1 region after the CFC test was attenuated. These 4VO-induced impairments were significantly alleviated by the administration of LEH (5 ml/kg, i.v.) during occlusion. Moreover, LEH did not alter hippocampal blood flow and tissue oxygen pressure during 4VO, but it did suppress hyperoxia after ischemia-reperfusion. These findings suggest that LEH, an artificial oxygen carrier, could be a novel therapeutic agent for brain dysfunction after acute cerebral ischemia.
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PMID:Liposome-encapsulated hemoglobin ameliorates impairment of fear memory and hippocampal dysfunction after cerebral ischemia in rats. 2107 36

In newborn infants, acute perinatal hypoxic/ischaemic events and associated hyperoxia/reperfusion injury frequently lead to devastating neonatal brain damage. The present report concerns a 3-week-old boy from Pakistan with d-transposition of the great arteries (d-TGA), prolonged and severe hypoxaemia, and multiresistant bacterial sepsis. The term newborn infant underwent public airline transportation to Europe and presented on the airport's runway with severe hypoxaemia (pulsoximetric oxygen saturations (SpO2) 17%) and systemic hypotension. The patient eventually underwent late balloon atrial septostomy, followed by a successful two-stage arterial switch operation. A clinical follow-up 3-5 years later revealed lack of cerebral dysfunction, adequate neurodevelopment, good biventricular function, regular coronary flow, as well as normal ECG, blood pressure and SpO(2). The findings may indicate the neonatal brain adjusts better to chronic, slowly worsening hypoxia than to acute hypoxia (eg, "birth asphyxia"), and also suggests a greater tolerance for chronic hypoxia in neonates vs adults.
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PMID:Arrival and survival of a 3-week-old boy from Pakistan with an arterial oxygen saturation of 17%. 2168 77

The abnormal level of O2 could disturb various neurochemical processes and even induce neural injury and brain dysfunction. In order to assess critical roles of O2 in the neurochemical processes, it is essential to perform in vivo monitoring of the dynamic changes of O2. In this study, we develop a new electrochemical method for selectively monitoring O2 in vivo, using platinized vertically aligned carbon nanotube (VACNT)-sheathed carbon fibers (Pt/VACNT-CFs) as the electrodes. The VACNT-sheathed CFs (VACNT-CFs) are produced via the pyrolysis of iron phthalocyanine (FePc) on the surface of CFs, followed by electrochemical deposition of platinum nanoparticles to form Pt/VACNT-CFs. The resulting Pt/VACNT-CF microelectrodes exhibit fast overall kinetics for the O2 reduction via a four-electron reduction process without the formation of toxic H2O2 intermediate. Consequently, effective and selective electrochemical methods are developed for the measurements of O2 in rat brain with the Pt/VACNT-CF microelectrodes, even in the presence of some species at their physiological levels, such as ascorbic acid, dopamine, uric acid, 5-hydroxytryptamine, and of the O2 fluctuation in rat brain in the early stage of global cerebral ischemia/reperfusion, mild hyperoxia, and hypoxia induced by exposing the animal, for a short time, to O2 and N2, respectively, and hindfeet pinch. The use of VACNT-CF as the support for Pt effectively improves the stability of Pt, as compared with the bare CF support, while the FePc pyrolysis ensures the VACNT-CFs to be reproducibly produced. Thus, this study offers a novel and reliable strategy for preparing new microelectrodes for in vivo monitoring of O2 in various physiological processes with a high sensitivity and selectivity.
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PMID:Platinized aligned carbon nanotube-sheathed carbon fiber microelectrodes for in vivo amperometric monitoring of oxygen. 2477 1