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Query: UMLS:C0242379 (lung cancer)
 71,905 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Using the double agar layer method of human tumor clonogenic assay, the anticancer effect of different combinations of anticancer drugs and interferons was tested on 3 lung cancer cell lines, PC-13, PC-14, and Calu-1. The anticancer drugs and the concentrations used in this study were cisplatin (1.0 microgram/mL), adriamycin (1.0 microgram/mL), mitomycin C (0.2 microgram/mL), VP-16 (5.0 micrograms/mL) and 5-FU (5.0 micrograms/mL). Three kinds of interferon, alpha, beta and gamma in 5,000 units/mL, were tested in combination or in sequence with other anticancer drugs on lung cancer cell lines. The results demonstrate an enhanced anticancer effect on PC-14 only with sequential or simultaneous combination of VP-16 with alpha, beta and gamma interferons; and on Calu-1, only with sequential use of adriamycin and beta-interferon. Our results indicate that there is no unique way of combining anticancer drugs and interferons which can obtain an enhanced anticancer effect on all lung cancer cell lines. The best combination of interferon and anticancer drugs seems to be influenced by the biological characteristics of the cancer cells.
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PMID:[Effect of combinations of anticancer drugs with interferons on human lung cancer cell lines evaluated by human tumor clonogenic assay]. 172 Jan 64

The cell spreading ability of human lung cancer cells on collagen substrata was examined in comparison with normal human tracheal epithelial cells. Plastic dishes or multiwells were coated with type I, III or IV collagen gel at a concentrate of 200 micrograms/cm2. Ninety per cent of the normal cells were round on all collagens. Adenocarcinoma RERF-LC-MS and VMRC-LCD cell lines and squamous cell carcinoma VMRC-LCP cell line, which metastasize weakly after intrasplenic transplantation in nude mice, spread relatively poorly. Adenocarcinoma, A549 and SK-LU-1 and squamous cell carcinoma Calu-1 cell lines, which were highly metastatic to liver, spread well. Adenocarcinoma ABC-1 cell line, which is moderately metastatic to liver in nude mice, spread moderately. On type III collagen, three adenocarcinoma cell lines (A549, ABC-1 and VMRC-LCD) gradually started to contract after initial spreading and became round at 24 h. These results suggest that there may be a correlation between the degree of malignancy of human lung cancer cells and their spreading ability on collagen substrata, and that the cell spreading ability may be regulated by type III collagen in some lung cancer cells.
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PMID:The spread of human lung cancer cells on collagens and its inhibition by type III collagen. 175 82

The lamins, an intranuclear class of intermediate filament proteins, are major structural proteins of the nuclear envelope. In the present study, the three abundant mammalian lamins (lamins A, B, and C) were observed to be present in roughly equivalent amounts in the Calu-1, Calu-3, H157, and SK-MES-1 non-small cell lung cancer lines. In the small cell lung cancer lines OH-1, OH-3, NCI-H82, NCI-H209, and NCI-H249, levels of lamin B were similar to those observed in the non-small cell lines, but the levels of lamins A and C were diminished by greater than or equal to 80%. The relationship between lung cancer phenotype and lamin expression was explored further in the NCI-H249 small cell line. Introduction of the v-rasH oncogene into this line gives rise to a cell line (NCI-H249rasH) with many features of large cell carcinoma of the lung (Falco, J. P., Baylin, S. B., Lupu, R., et al. J. Clin. Invest., 85: 1740-1745, 1990). Concomitant with the v-rasH-induced change in phenotype, a greater than 10-fold increase in the amounts of lamins A and C was observed. Levels of the cytoplasmic intermediate filament protein vimentin also increased. In contrast, levels of a variety of nonlamin nuclear polypeptides including topoisomerase I, topoisomerase II, poly(ADP-ribose) polymerase, and the nucleolar protein B23/nucleophosmin did not change. Comparison of polyadenylated RNA from NCI-H249 and NCI-H249rasH cells on Northern blots revealed similar levels of the mRNA for lamin B but higher levels of the mRNAs for lamins A and C in the v-rasH-expressing cell line. These observations provide evidence for differences in nuclear envelope structure in histologically different neoplastic cells derived from the same epithelial cell system and suggest that differences in lamina structure result from phenotype-specific differences in lamin gene expression.
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PMID:Differential expression of nuclear envelope lamins A and C in human lung cancer cell lines. 198 76

Corticosteroid levels were studied in the plasma of athymic mice implanted with human breast tumor cells, either from MCF-7 or ZR-75-1 cell lines. There was a highly significant decrease in plasma corticosterone levels in the mice implanted with these tumor cells. There was no significant effect on corticosterone of GW 39 colon cancer cells, LS 174T colon cancer cells, or Calu-3 lung cancer cells.
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PMID:Diminished corticosterone levels in nude mice implanted with MCF-7 or ZR-75-1 human breast tumor cells. 280 57

Phototoxicity of benzoporphyrin derivative (BPD) has been tested in vitro and compared with that of hematoporphyrin (HP). After 1-hour activation with visible light, BPD was 10 times more cytotoxic than HP toward human adherent cell lines: A549 lung cancer, Calu-1 lung carcinoma, and CCD-19Lu normal lung, killing 100% of cells at the concentration of 70 ng/ml. Under the same conditions, BPD was 10-70 times more cytotoxic than HP toward nonadherent cells and cell lines. Tested were human leukemia cell lines HL60, K562, and KG1, normal human lymphocytes, and mouse mastocytoma cell line P815. The concentrations required to kill 100% of cells varied between 10 and 500 ng BPD/ml and between 0.2 and 10 micrograms HP/ml. The difference between the nonadherent cell lines in respect to their sensitivity to phototoxicity of both BPD and HP seemed to be related to the cell sizes, with the smallest cells being the most vulnerable. The most attractive characteristic of BPD in addition to its powerful phototoxicity is its maximum absorption around 700 nm, which is in the range of wavelengths penetrating tissues the best. This characteristic alone could make BPD a drug of choice in cancer photodynamic therapy when the safety of its use is ensured. Preliminary tests in vivo have shown that DBA/2J mice can tolerate a single ip injection of 20-60 micrograms BPD as well as the same dose of HP. The biodistribution and toxicity studies of BPD are under way in our laboratory.
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PMID:Preliminary studies on a more effective phototoxic agent than hematoporphyrin. 348 Mar 84

A modified leukocyte adherence inhibition (H-LAI) assay was used to study immunological factors in serum from lung cancer patients. In this test, 0.25% serum was added to the assay system, together with tumor antigen and trypsinized leukocytes from control persons. Extracts from a human lung cancer cell line (Calu-1) and a human breast cancer cell line (MCF-7) were used as antigens. The results obtained were compared with data found with the original hemocytometer (C-LAI) assay. Of 21 lung cancer patients studied, 20 (95%) gave a positive response in both the H-LAI and the C-LAI assay systems against Calu-1 antigen. Only 1 of the patients gave a positive response in the H-LAI system against MCF-7 antigen, while 3 patients (14%) responded in the C-LAI assay. None of the 14 control persons tested gave a positive response. While the C-LAI assay was limited to the use of fresh blood, the H-LAI system was performed on small amounts of serum. The serum could be stored in the frozen state for a long time period. The results indicate that the H-LAI assay possesses at least the same sensitivity and specificity as the original C-LAI test.
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PMID:Immune responses in lung cancer patients measured by a modified leukocyte adherence inhibition test using serum. 698 87

A modified leukocyte adherence inhibition (H-LAI) assay has recently been developed in which 0.25% serum from the patient is added to the assay system in combination with the relevant antigen. Trypsinized leukocytes from control persons are used as indicator cells. In the present work, the nature of the humoral factor in serum from breast and lung cancer patients is studied. 3.5 M KCl extracts from the cell lines MCF-7 and Calu-1 were used as breast and lung cancer antigen, respectively. It was found that the humoral factor involved in the H-LAI response was precipitated from the sera by addition of ammonium sulphate to 50% saturation. This factor could be removed by passage through an affinity column with the relevant antigen bound to the matrix. Stable complexes were formed between the humoral factor and the relevant antigen, and could be precipitated by polyethylene glycol. When different anti-immunoglobulins were added to the sera, the humoral factor was specifically removed by addition of anti-IgG antibodies. The data presented indicate that the humoral factor in sera from patients with breast and lung cancer is antitumor antibodies of IgG nature.
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PMID:Role of circulating antibodies in the humoral leukocyte adherence inhibition response of lung and breast cancer patients. 701 72

The hemocytometer leukocyte adherence inhibition technique was used to study cell-mediated immuno-activity of patients with lung cancer. KCl extracts (3.5 M) from the lung cancer cell line Calu-1 and the breast cancer cell line MCF-7 were used as antigens. Of 138 patients with lung cancer, 85% showed a positive response against the Calu-1 antigen. The response was independent of the histological type of the tumor and was the same among untreated patients, patients undergoing different types of treatment and patients who died within 3 months after blood collection. Twenty-five percent of the untreated lung cancer patients also reacted against the breast cancer antigen. Among lung cancer patients undergoing different types of treatment, 36% reacted while 50% of the patients who died within 3 months after blood collection reacted against the breast cancer antigen.
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PMID:Leukocyte adherence inhibition assay in human pulmonary neoplasia. 704 65

Polymerase chain reaction amplification and BstNI endonuclease digestion were performed on DNA isolated from cell lines that were either homozygous (SW480, A549) or heterozygous (Calu 1, SK-LU-1, A427) for K-ras codon 12 mutations. Polyacrylamide gel electrophoresis showed that both mutant and wildtype (WT) bands were present in Calu-1, SK-LU-1, and A427 cell DNA; only the mutant bands were observed with SW480 and A549 DNA. The percentages of mutant and WT fragments were measured using capillary electrophoresis (CE). Integration of mutant and WT peaks showed that the percentages of mutant alleles in Calu-1, SK-LU-1, and A427 cell lines were 73, 84, and 72, respectively. The sensitivity of the original BstNI assay for K-ras codon 12 in conjunction with analysis by CE was also tested by a series of titration experiments using one- and two-stage amplification-BstNI digestion protocols. CE was used to generate a calibration curve. The mutant allele was detected and the quantity was measured in the 1:100 and 1:10,000 dilutions in the one- and two-stage analysis, respectively. Four human lung adenocarcinomas were also analyzed. Two of these were homozygous normal, whereas the other two contained 63 and 32% codon 12 mutant alleles. These results showed that CE can separate and quantitate BstNI fragments containing K-ras codon 12 mutations. The high sensitivity and quantitative features of CE should enable detection and quantitation of mutant K-ras alleles in premalignant lung lesions, as well as exfoliated cells collected by cytology from persons at risk for lung cancer.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Detection and quantitation of mutant K-ras codon 12 restriction fragments by capillary electrophoresis. 771 62

We developed a three-dimensional type I collagen gel cell culture system that allows coculturing of human MG-63 osteoblast-like cells and various human cancer cells. Inoculation of human PC-3 metastatic prostate cancer cells into this type I collagen gel containing human MG-63 osteoblast-like cells produced an osteoblastic-like reaction that presented as an increased number of MG-63 cells and increased density of type I collagen around MG-63 cells adjacent to inoculated PC-3 cells by microscope analysis. Under identical experimental conditions, inoculation of cell-free medium, human KLE endometrial adenocarcinoma cells, and Calu-1 lung cancer cells did not produce this blastic-like reaction. In situ hybridization documented the uniform expression of insulin-like growth factor I (IGF-I) and of urokinase-type plasminogen activator (uPA) mRNA in MG-63 and PC-3 cells separately cultured in this substrata. The uniform expression of uPA was also documented by immunocytochemistry using a monoclonal and a polyclonal antihuman uPA antibody. The relative expression of uPA was higher in PC-3 cells than in MG-63, KLE, and Calu-1 cancer cells. We conclude that this novel cell culture system may become a useful model to study the pathophysiology of the osteoblastic reaction in vitro.
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PMID:Three-dimensional type I collagen gel system for the study of osteoblastic metastases produced by metastatic prostate cancer. 786 32


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