Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0239946 (liver fibrosis)
 8,268 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The activities of enzymes collagen peptidase (CP), monoaminoxidase (MAO) and N-acetyl-beta-D-glucosaminidase (beta-NAG) in the serum are related to the development of hepatic fibrosis. These enzymes were determined in the sera of 121 liver-biopsied patients who were subdivided by morphological criteria into 4 different grades of fibrosis (0 to greater than or equal to 3). CP shows the best correlation with the extent of fibrosis. beta-NAG, indeed, bears of relationship to the morphologically proven extent of fibrosis, however, significant increases in activity are also encountered in patients with liver diseases, but without liver fibrosis. The validity of these fibrosis markers is calculated in order to differentiate between low- and high-grade fibrosis. With a sensitivity of 86% and a specificity of 92%, CP possesses the best predictive value of 85.7%. The results can still be improved by the combination of CP and beta-NAG.
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PMID:Enzyme activities of collagen peptidase (CP), monoaminoxidase (MAO) and N-acetyl-beta-D-glucosaminidase (beta-NAG) as fibrosis marker in chronic liver diseases. 283 53

The mortality of liver cirrhosis has doubled during the past two decades and is still increasing in most of the European countries. One important feature and precursor of liver cirrhosis is liver fibrosis. Its aetiology includes a wide spectrum of well known causes, in Europe most frequently alcohol, virus infection, and chemical agents. The pathomechanism of liver fibrosis is unknown. Diagnostic and therapeutic approaches for early detection and treatment have been recently developed applying the results of pathobiochemical, cellular and clinical research. The composition of the excess hepatic connective tissue suggests the involvement of myofibroblasts and shows similarities to atherosclerotic plaques and lung fibrosis. The isolation, purification and cultivation of cells from liver biopsies offers new avenues for the study of fibroplastic cells. Clinical studies are now facilitated, since products of the collagen synthetic pathway - procollagen peptides - can be measured in serum with a sensitive radioimmunoassay. Further prospective studies including additional parameters of fibroplasia, such as N-acetyl-beta-D-glucosaminidase, lysyl-oxidase and prolyl-hydroxylase will have to demonstrate the diagnostic value of such methods. Even today, they should be applied in therapeutic trials of chronic fibrotic liver diseases. Better knowledge of the molecular regulation of connective tissue and the use of new animal models and cellular systems support a successful search for new therapeutic tools. Above all, limitation of alcohol consumption, vaccination for viral hepatitis and elimination of chemical agents offer the prevention of fibrosis, which calls for major efforts on a nation-wide scale.
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PMID:[Liver fibrosis. 2]. 610 5

The simultaneous determination of the catalytic activities in serum of monoamine oxidase (EC 1.4.3.4) and N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30) was performed in patients with various non-liver diseases, acute hepatitis and fibroproliferative liver disorders (cirrhosis and fibrosis) and the predictive values of the positive (both activities are pathologically elevated) and negative test results (normal activity of monoamine oxidase and/or N-acetyl-beta-D-glucosaminidase) were estimated. It was found that the incidence of the positive result is extremely low (0.024, 5/207) in patients suffering from a great variety of non-liver and liver diseases (except cirrhosis) but rather great in liver cirrhotic subjects (0.44, 18/41). A fraction of only 0.07 of liver fibrotic patients had a positive test result. Based on these data the estimated predictive value of the positive result for liver cirrhosis at a prevalence of 0.03 is 0.47. This value increases strongly with higher prevalence of cirrhosis (population preselected for chronic liver diseases). The negative predictive value for cirrhosis and the positive value for fibrosis are low. Thus, the probability of the presence of cirrhosis in patients with suspected chronic liver diseases is great in cases of abnormally high activities of both monoamine oxidase and N-acetyl-beta-D-glucosaminidase. Negative test results (normal catalytic activities of one or both enzymes), however, do not prove the absence of liver cirrhosis and/or liver fibrosis.
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PMID:The diagnostic potential of the combined determination of serum monoamine oxidase and N-acetyl-beta-D-glucosaminidase for fibroproliferative liver diseases. 685 16

The catalytic activities of 4 glycosidases (hyaluronate-4-glycanohydrolase (EC 3.2.1.35), beta-N-acetyl-D-glucosaminidase (EC 3.2.1.30), beta-glucuronidase (EC 3.2.1.31), alpha-L-iduronidase (EC 3.2.1.76)), of the arylsulphatases A and B (EC 3.1.6.1) and of the protease cathepsin D (EC 3.4.23.5) were measured in extracts from hepatocytes and non-parenchymal cells and in serum during the development of thioacetamide-induced rat liver fibrosis (22 weeks). In non-parenchymal liver cells the catalytic activities of beta-N-acetyl-D-glucosaminidase, beta-glucuronidase, alpha-L-iduronidase and cathepsin D were increased significantly during chronic liver damage, but that of hyaluronate-4-glycanohydrolase was reduced by 40 to 65% during the period of application of thioacetamide. The catalytic activities of the arylsulphatases were lowered by 65% compared to control values in the 12th week but with advancing liver damage the catalytic activities returned to nearly normal values. Parenchymal cells of rats, which had been liver-damaged for 6 months, contained strongly elevated activities of beta-glucuronidase, beta-N-acetyl-D-glucosaminidase, arylsulphatases A and B, and cathepsin D but only slightly increased activities of hyaluronate-4-glycanohydrolase and alpha-L-iduronidase, respectively. In the serum of liver-damaged rats the activity of alpha-L-iduronidase was strongly elevated, while that of N-acetyl-beta-D-glucosaminidase was only slightly increased. The activities of beta-glucuronidase and of arylsulphatases A and B were decreased during the whole period of treatment. The catalytic functions of hyaluronate-4-glycanohydrolase and of cathepsin D, respectively, were decreased initially, but both enzyme activities were elevated during the more advanced stages of long term thioacetamide treatment.
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PMID:Changes in the catalytic activities of proteoglycan-degrading lysosomal enzymes in parenchymal and non-parenchymal liver cells and in serum during the development of experimental liver fibrosis. 687 76

The mortality of liver cirrhosis has doubled during the past two decades and is still increasing in most of the European countries. One important feature and precursor of liver cirrhosis is liver fibrosis. Its aetiology includes a wide spectrum of well known causes, in Europe most frequently alcohol, virus infection, and chemical agents. The pathomechanism of liver fibrosis is unknown. Diagnostic and therapeutic approaches for early detection and treatment have been recently developed applying the results of pathobiochemical, cellular and clinical research. The composition of the increased hepatic connective tissue suggests the involvement of myofibroblasts and shows similarities to atherosclerotic plaques and lung fibrosis. The isolation, purification and cultivation of cells from liver biopsies offers new avenues for the study of fibroplastic cells. Clinical studies are now facilitated, since products of the collagen synthetic pathway -- procollagen peptides--can be measured in serum with a sensitive radioimmunoassay. Further prospective studies including additional parameters of fibroplasia, such as N-acetyl-beta-D-glucosaminidase, lysyloxidase and prolylhydroxylase will have to demonstrate the diagnostic value of such methods. Even today, they should be applied in therapeutic trials of chronic fibrotic liver diseases. Better knowledge of the molecular regulation of connective tissue, and the use of new animal models and cellular system support a successful search for new therapeutic tools. Above all, limitation of alcohol consumption, vaccination for viral hepatitis and elimination of chemical agents offer the prevention of fibrosis, which calls for major efforts on a nation-wide scale.
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PMID:[Liver fibrosis. 1 (author's transl)]. 745 64