Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0220723 (PCA)
 4,687 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Homocytotrophic antibodies (HCA) were produced in rats by primary injection of streptokinase-streptodornase (SK-SD) after the antigen was absorbed on alminum hydroxide gel. The properties of rat SK-SD-specific HCA were qualitatively similar to SK-SD skin-sensitizing antibodies found in sera of human beings, namely, homocytotropic activity (48 h homologous PCA reaction), heat lability at 56 degrees C, and inactivation by reduction with 2-mercaptoethanol followed by alkylation with iodoacetamide. In neutralization tests, D-SK-SD, a low molecular hapten-like substance contained in SK-SD preparation, absorbed the antibody against ND-SK-SD antigen-specifically. The neutralizing activity mostly resided in fraction 1 of D-SK-SD separated through DEAE-Sephadex A-25 ion-exchange column, opposing the inhibitory activity of fraction 3 on lymphocyte transformation. The possible implication of these findings in the immunological treatment of streptococcal disorders is discussed.
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PMID:Induction of rat homocytotropic antibody against streptokinase-streptodornase (SK-SD) and specific neutralization of the antibody by low molecular weight component of SK-SD. 6 5

It appears to be significant for the analytical studies of drug allergy that a comparison is made among inbred strains of mice, with different H-2 haplotypes with respect to the antibody response to low-molecular compounds. Such studies have been demonstrated by several investigators using high-molecular antigens, such as the synthetic polypeptides and natural proteins. The present experiments were undertaken, therefore, to clarify the differences in antibody responses to a low-molecular compound, TNBS in inbred strains of mice. Mice of four different strains, A/J (H-2a), C57BL/6 (H-2b), DBA/2 (H-2d) and C3H/He (H-2k) were immunized with TNBS (30 & 300 micrograms/body) plus FCA by the routes of ip or sc. Responses of IgG and IgE antibody productions were estimated by HA in serum samples treated with 2-ME and rat PCA reaction in untreated serum samples, respectively. As the results, DBA/2 (H-2d) mice showed high responses on IgE antibody production whereas C3H/He (H-2k) mice on IgG antibody production. A/J (H-2a) mice displayed high responses both on IgG and IgE antibody productions. C57BL/6 (H-2b) mice denoted low to intermediate responses both on IgG and IgE antibody productions. The above phenomenon may be interpreted by the fact that H-2a haplotype of A/J mice has a crossover on K region-E alpha subregion of H-2k and S.D regions of H-2d.
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PMID:[Differences in IgG and IgE antibody responses to a low-molecular compound in inbred strains of mice]. 205 60

Dogs immunized with o-DNCP-BSA or eggs of Toxocara canis developed anaphylactic antibodies other than IgE. The antibodies were heat-resistant at 56 degrees C for 2 hours, not reduced by 2-mercaptoethanol and PCA and P-K reactivity was only observed at 3-4 h sensitized sites of recipient skin. High titres of antigen-specific IgGd were determined by means of an ELISA technique.
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PMID:Induction of non-IgE anaphylactic antibodies in dogs. 397 74