Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0220723 (PCA)
 4,687 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Since substance P (SP) has been demonstrated to coexist with serotonin (5-HT) in the same population of neurons in the descending raphe system, we have studied the possibility of interactions between these neurotransmitters in other brain areas. Brain nuclei were punched from frozen 300-micron slices of rat brain and extracted with 0.1 M HCIO4 or 2 M acetic acid prior to assay, respectively, of 5-HT content by HPLC with electrochemical detection or SP content by specific radioimmunoassay. Ten days after injection of rats with the 5-HT neurotoxin P-chloroamphetamine (PCA, 10 mg/kg, B.W., i.p.) or 3 days after 5-HT synthesis blockade with p-chlorophenylalanine (PCPA, 300 mg/kg, B.W., i.p.), the 5-HT content of all brain nuclei studied was reduced by means of, respectively, 50% and 81%. In PCA-treated animals, the SP content of the periaqueductal grey matter was significantly increased; PCPA treatment caused, in addition, large increases in the SP content of five other brain nuclei. Blockade of 5-HT receptors by methysergide (15 mg/kg for 5 days) did not significantly change 5-HT levels or turnover, but resulted in 50-200% increases in the SP content of 10 of the 28 brain nuclei studied. Significant decreases in the SP content of numerous areas were seen following treatments (pargyline 30 mg/kg, alone or in combination with 5-hydroxytryptophan, 60 mg/kg) that simultaneously increased 5-HT levels. These results illustrate the modulation of distinct SP-containing systems of the rat brain by perturbation of central serotoninergic pathways and indicate a reciprocal relationship between the SP and 5-HT concentrations of numerous brain nuclei, in particular n. striae terminalis, n. raphe dorsalis, n. accumbens, n. septi, substantia grisea centralis, and n. raphes medianus.
J Neurochem 1983 Sep
PMID:Perturbation of rat brain serotonergic systems results in an inverse relation between substance P and serotonin concentrations measured in discrete nuclei. 619 16

REV 3164 has been evaluated in a variety of intact rodent models to reveal potential utility in the prophylactic treatment of asthma. REV 3164 was found a potent, orally active inhibitor of rat (IgE) passive cutaneous anaphylaxis (PCA, ED50 = 0.9 mg/kg). By contrast, at 50-200 mg/kg p.o., it did not affect guinea-pig (IgG1) PCA. In PCA rats, both REV 3164, 1-36 mg/kg i.p., and the known inhibitor of mast cell mediator release, disodium cromoglycate (DSCG), 2-54 mg/kg i.p., blocked cutaneous wheals caused by i.v. antigen challenge but not by intradermal serotonin or histamine. Neither REV 3164 (0.1-10 mg/kg i.p.) nor DSCG (2-54 mg/kg i.p.) affected Compound 48/80-induced wheals. REV 3164 (0.01-1 mg/kg i.v. or 10 mg/kg i.p.) abolished rat (IgE) passive lung anaphylaxis (PLA, ED50 = 0.05 mg/kg i.v. for inhibition of elevated airway resistance). At 10 mg/kg i.p., REV 3164 did not affect active lung anaphylaxis in guinea-pigs pharmacologically manipulated to enhance the production and action of slow reacting substance of anaphylaxis (SRS-A), nor did it exhibit anticholinergic activity in the rat. REV 3164 (100 mg/kg i.p.) did not protect conscious guinea-pigs from histamine aerosol-induced collapse. It is concluded that REV 3164 is an oral inhibitor of IgE-dependent immediate hypersensitivity in the rat with biological activities in rats and guinea-pigs similar to DSCG.
Arch Int Pharmacodyn Ther 1984 Sep
PMID:In vivo anti-allergic and bronchopulmonary pharmacology of REV 3164 in rats and guinea-pigs. 649 6

CB-1A chemical characterization comprised the determination of nitrogen (Kjeldhal: 15.5%: amino acid composition: 17.5%), total (6.8%) and reducing (1.6%) carbohydrates, amino acid composition (Lys4, His1, Arg11, Asp3, Thr1, Ser7, Glu16, Pro2, Gly5, Ala3, Cys6, Val3, Met1, Ile3, Leu4, Tyr1, Phe1; 82 residues). Its behaviour under several TCA concentrations, ultraviolet absorption of native and oxidized CB-1A under several solvents as well as its enzymatic susceptibility were explored. Physicochemical parameters such as MW (protein moiety - composition: 9.642 Da, column: 9.431 Da: glycoprotein-10.345) Da; E1%1cm,220mm = 49.7 E1%1cm,595mm = 29.7: partial specific volume (anhydrous: 0.703 cm3/g; hydrated; 0.924 cm3/g); Stokes radius (15.1 A); hydration water (22%) and frictional ratio (1:10) were determined. Calculated limit molecular dimensions (semi-axis of revolution: 11.4 to 19.8 A) and equatorial radius (13.2 to 17.5 A suggest dendency to sphericity. CB-1A showed allergenicity by PCA. Fourteen components were detected by IEF.
An Acad Bras Cienc 1984 Sep
PMID:Chemical and physicochemical characterization of CB-1a, an allergenic fraction isolated from castor bean (Ricinus communis L.). 650 26

This report describes an extremely rare case of occupational allergy that developed in a frog handler. A 31-year-old female laboratory technician developed itching and urticaria one year after she began handling frogs and extracting their brains in the laboratory. Nine years later she noticed swelling of the right hand, stridor and dyspnea when she mistakenly injected her finger with a needle contaminated with extracts of frog brain. Specific IgE antibody to frog extracts was demonstrated by RAST and by P-K testing. However, no specific IgG antibody was found by agar gel diffusion or in heterologous PCA testing using guinea pigs. We suggest that allergic symptoms in this case were due to the development of Type I allergic reactivity to frog antigens.
Ann Allergy 1983 Sep
PMID:A new occupational allergy due to frogs. 660 74

Two hundred eight white women, aged 60 to 69 years, had acquired 218 upper or lower full dentures. Each woman's smoking habits and current osteoporosis severity (percent cortical area [PCA] at metacarpal midshaft) were compared with the age at which she had acquired each full denture. Among osteoporotic women (PCA less than 70%) who still had their natural teeth at age 50 years, 44% had required a new full denture before age 60 compared with 15% of nonosteoporotic women (PCA greater than 80). Different denture requirements between these groups had not existed before age 50 but had continued after age 60. Fifty-two percent of smokers, 26% of nonsmokers, and only 8% of nonosteoporotic nonsmokers had required dentures since age 50. These observations strongly suggest that middle-aged women may be more likely to retain their teeth if they avoid smoking and undertake a program effective in preventing progression of osteoporosis.
Arch Intern Med 1983 Sep
PMID:Postmenopausal tooth loss. Contributions to edentulism by osteoporosis and cigarette smoking. 661 88

In some allergic sera, 2-hr PCA responses can be due to IgE class antibody. In other sera where 2-hr PCA responses are not seen, removal of the IgG fracton allows expression of this 2-hr latency PCA response, suggesting an initial competition for binding sites between IgG and IgE, or that IgG can act as a blocking antibody, preventing antigen from reaching the cell-bound IgE.
Clin Allergy 1980 Sep
PMID:Short and long latency skin sensitizing antibody in passive cutaneous anaphylaxis in the monkey. 696 43

The present investigation assessed PCA toxicity at 0.0, 5.0, and 10.0 mg/kg, in both social (4 rats per cage) and non-social (acrylic tube-restraint or tube restraint-plus-tail shock) circumstances with 16 rats per drug-environment condition. The results indicated that no dose of PCA alone yielded mortality under individual housing, and similarly no environmental circumstance by itself yielded mortality in the absence of PCA. However, various drug-environment interactions produced a dose-related enhancement of PCA toxicity. For both 5.0 mg/kg and 10 mg/kg parachloroamphetamine dose levels, restraint-plus-shock generated the highest percent mortality, followed by restraint-only, with conspecific aggregation producing a mortality incidence lower still. Further, the mortality displayed under each of these environmental conditions was greater for the 10.0 mg/kg PCA treatment than for the 5.0 mg/kg treatment. The results are discussed in terms of the relative aversiveness of the environmental setting and it is suggested that stress-related drug toxicity may be further analyzed in non-social settings. It is proposed that toxic environment-PCA interactions may result from altered cardiovascular and/or thermoregulatory processes, mediated by enhanced catecholaminergic activity.
Pharmacol Biochem Behav 1982 Sep
PMID:Environmental determinants of parachloroamphetamine toxicity in rats. 714 48

Serum sickness was induced in rats by a modification of previously described methods avoiding i.v. administration of the antigen. All the animals developed a progressive disease characterized by an initial pattern of deposition of IC in the mesangium followed by the appearance of GBM deposits. This change in the deposition of IC was associated with the onset of massive proteinuria and a fall in the titre of precipitating antibodies. Simultaneously, specific desensitization of platelets for a rat PAF could be demonstrated and platelet aggregates were seen in the glomeruli. The presence of homocytotropic IgGa anti-ovalbumin antibodies in rat sera during the induction of the disease was demonstrated by 2 hr PCA. Accordingly, this antibody together with the antigen ovalbumin induced the release of histamine from peritoneal mast cells, suggesting that a similar mechanism might occur in vivo during the induction of the disease. Rat PAF and beta glucuronidase could be obtained from peritoneal macrophages under similar conditions to those required for the release of histamine. The data support a role for inflammatory mediators in the increase in vascular permeability needed for the deposition of IC in the GBM and provide evidence for a new role of macrophages and PMNs in glomerular pathology in contributing to an increase in permeability of GBM.
Clin Exp Immunol 1982 Sep
PMID:Rat serum sickness: possible role of inflammatory mediators allowing deposition of immune complexes in the glomerular basement membrane. 717 98

The perfusion effluent from isolated rat lungs caused relaxations of superfused strips of bovine (BBBCA) and pig (PCA) coronary arteries. The effluent from hamster lungs had similar effect on BCA. Bolus injections of arachidonate (AA, 10-20 microgram) into rat lungs caused further relaxations of superfused BCA and contractions of rat stomach strip (RSS). AA injections into guinea pig lungs resulted in dose dependent contractions of BCA and PCA. Similar dose dependent contractions of PCA and RSS were seen in hamster experiments, however the responses of BCA varied with the AA dose. Infusion of AA (5-25 microgram/min) into guinea pig lungs resulted in dose dependent contractions of superfused PCA and BCA. In hamster experiments AA infusion (5 microgram/min) caused a small relaxation of BCA, but had no effect on PCA. When 14C-AA was injected into the pulmonary circulation, the amounts of metabolites were greater in the perfusion effluent from hamster than from rat lungs. In rat lungs 6-oxo-PGF1 alpha was one of the main metabolites. In contrast, in hamster lungs this metabolite represented only a small part of the total metabolite formation. Thromboxane B2 was one of the main metabolites formed in hamster lungs and its rate of formation increased greatly with increasing AA doses. The results indicate that the metabolite pattern of arachidonate in isolated rat lungs is different from that in hamster and guinea pig lungs. The total rate of AA metabolism in rat lungs is smaller than in hamster lungs.
Prostaglandins Med 1980 Sep
PMID:The metabolism of arachidonic acid in isolated hamster, rat and guinea pig lungs. 741 49

Biosensors sensitive for in vivo monitoring of serotonin (5-HT) in the CNS by differential normal pulse voltammetry were constructed by coating treated multicarbon fiber electrodes (mCFEs) with Nafion (N-mCFE). In vitro sensitivities of mCFE and N-mCFE were compared in solutions ranging from 5 nM to 20 microM of uric acid (UA), 5-hydroxyindoleacetic acid (5-HIAA), and 5-HT. The mCFEs were three to seven times less sensitive for 5-HIAA or UA than for 5-HT. Nafion treatment dramatically decreased sensitivity for 5-HIAA and UA of N-mCFEs (approximately 10(3) times), whereas it remained in the nanomolar range for 5-HT. In vivo, in the dorsal horn of the lumbar spinal cord of anesthetized rats, the monoamine oxidase inhibitor clorgyline (10 mg/kg i.p.) produced a reduction (55 +/- 3% at 180 min) of peak 3 of oxidation current (characteristic of 5-hydroxyindoles) monitored with mCFEs, but with N-mCFEs (in this latter case the peak was termed 3N) peak 3N increased to 135 +/- 5% at 180 min. The 5-HT release-inducer p-chloroamphetamine (PCA; 6 mg/kg i.p.) induced a slight (12 +/- 3% at 150 min) decrease in peak 3 measured with mCFEs, whereas with N-mCFEs PCA induced a rapid increase of peak 3N (137 +/- 6% at 90 min). The xanthine oxidase inhibitor allopurinol (10 mg/kg i.p.) produced a decrease (30 +/- 3% at 180 min) in peak 3 (mCFEs), but peak 3N (N-mCFEs) was not affected (106% at 180 min). After pretreatment with allopurinol, PCA also produced an increase (135 +/- 6% at 90 min) in peak 3N.(ABSTRACT TRUNCATED AT 250 WORDS)
J Neurochem 1995 Sep
PMID:In vivo electrochemical monitoring of serotonin in spinal dorsal horn with Nafion-coated multi-carbon fiber electrodes. 754 31


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>